A novel method for detecting HIV-1 by non-radioactive in situ hybridization: application of a peptide nucleic acid probe and catalysed signal amplification

A novel in situ hybridization (ISH) method for detecting human immunodeficiency virus‐1 (HIV‐1) was developed by applying a peptide nucleic acid (PNA) probe and a catalysed signal amplification (CSA) method. The PNA probe used in the present study possessed 15 base sequences of the HIV‐1 protease ge...

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Veröffentlicht in:	The Journal of pathology 2001-05, Vol.194 (1), p.130-135
Hauptverfasser:	Murakami, Takaya, Hagiwara, Tomoko, Yamamoto, Kazuko, Hattori, Junko, Kasami, Masako, Utsumi, Makoto, Kaneda, Tsuguhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Acquired Immunodeficiency Syndrome - diagnosis Adult AIDS catalysed signal amplification Catalysis CD4-Positive T-Lymphocytes - virology Cell Line HIV-1 HIV-1 - isolation & purification Human immunodeficiency virus 1 Humans in situ hybridization In Situ Hybridization - methods Male Middle Aged Nucleic Acid Probes Paraffin Embedding peptide nucleic acid peptide nucleic acid probe Peptide Nucleic Acids
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