Effects of oxygenation and flow on the viability and function of rat hepatocytes cocultured in a microchannel flat-plate bioreactor
The goal of this study was to investigate the viability and synthetic function of rat hepatocytes cocultured with 3T3‐J2 fibroblasts in a small‐scale microchannel flat‐plate bioreactor with and without an internal membrane oxygenator under flow. Bioreactor channel heights ranged between 85 and 500 μ...
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| Veröffentlicht in: | Biotechnology and bioengineering 2001-06, Vol.73 (5), p.379-389 |
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| creator | Tilles, Arno W. Baskaran, Harihara Roy, Partha Yarmush, Martin L. Toner, Mehmet |
| description | The goal of this study was to investigate the viability and synthetic function of rat hepatocytes cocultured with 3T3‐J2 fibroblasts in a small‐scale microchannel flat‐plate bioreactor with and without an internal membrane oxygenator under flow. Bioreactor channel heights ranged between 85 and 500 μm and medium flow rates ranged between 0.06 and 4.18 mL/min. The results showed that the bioreactor without the oxygenator resulted in significantly decreased viability and function of hepatocytes, whereas hepatocytes in the bioreactor with internal membrane oxygenator were able to maintain their viability and function. The shear stress calculations showed that, at lower wall shear stresses (0.01 to 0.33 dyn/cm2), hepatocyte functions, measured as albumin and urea synthesis rates, were as much as 2.6‐ and 1.9‐fold greater, respectively, than those at higher wall shear stresses (5 to 21 dyn/cm2). Stable albumin and urea synthesis rates for 10 days of perfusion were also demonstrated in the bioreactor with internal membrane oxygenator. These results are relevant in the design of hepatocyte bioreactors and the eventual scaling‐up to clinical devices. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 379–389, 2001. |
| doi_str_mv | 10.1002/bit.1071 |
| format | Article |
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Bioreactor channel heights ranged between 85 and 500 μm and medium flow rates ranged between 0.06 and 4.18 mL/min. The results showed that the bioreactor without the oxygenator resulted in significantly decreased viability and function of hepatocytes, whereas hepatocytes in the bioreactor with internal membrane oxygenator were able to maintain their viability and function. The shear stress calculations showed that, at lower wall shear stresses (0.01 to 0.33 dyn/cm2), hepatocyte functions, measured as albumin and urea synthesis rates, were as much as 2.6‐ and 1.9‐fold greater, respectively, than those at higher wall shear stresses (5 to 21 dyn/cm2). Stable albumin and urea synthesis rates for 10 days of perfusion were also demonstrated in the bioreactor with internal membrane oxygenator. These results are relevant in the design of hepatocyte bioreactors and the eventual scaling‐up to clinical devices. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 379–389, 2001.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.1071</identifier><identifier>PMID: 11320508</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>3T3 Cells ; Animals ; bioartificial liver ; Biological and medical sciences ; Bioreactors ; Biotechnology ; Cell Survival ; Coculture Techniques ; Female ; flat plate ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Hepatocytes - cytology ; Hepatocytes - metabolism ; Industrial applications and implications. Economical aspects ; Mice ; microfabrication ; Miscellaneous ; Oxygen - metabolism ; perfusion ; Rats ; Rats, Inbred Lew ; shear stress</subject><ispartof>Biotechnology and bioengineering, 2001-06, Vol.73 (5), p.379-389</ispartof><rights>Copyright © 2001 John Wiley & Sons, Inc.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5181-d9c9ba62d01a4bb4c9d15081d7a940a62662c8cd6af02ab54f6a1c2bd94af17f3</citedby><cites>FETCH-LOGICAL-c5181-d9c9ba62d01a4bb4c9d15081d7a940a62662c8cd6af02ab54f6a1c2bd94af17f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.1071$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.1071$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1102370$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11320508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tilles, Arno W.</creatorcontrib><creatorcontrib>Baskaran, Harihara</creatorcontrib><creatorcontrib>Roy, Partha</creatorcontrib><creatorcontrib>Yarmush, Martin L.</creatorcontrib><creatorcontrib>Toner, Mehmet</creatorcontrib><title>Effects of oxygenation and flow on the viability and function of rat hepatocytes cocultured in a microchannel flat-plate bioreactor</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>The goal of this study was to investigate the viability and synthetic function of rat hepatocytes cocultured with 3T3‐J2 fibroblasts in a small‐scale microchannel flat‐plate bioreactor with and without an internal membrane oxygenator under flow. Bioreactor channel heights ranged between 85 and 500 μm and medium flow rates ranged between 0.06 and 4.18 mL/min. The results showed that the bioreactor without the oxygenator resulted in significantly decreased viability and function of hepatocytes, whereas hepatocytes in the bioreactor with internal membrane oxygenator were able to maintain their viability and function. The shear stress calculations showed that, at lower wall shear stresses (0.01 to 0.33 dyn/cm2), hepatocyte functions, measured as albumin and urea synthesis rates, were as much as 2.6‐ and 1.9‐fold greater, respectively, than those at higher wall shear stresses (5 to 21 dyn/cm2). Stable albumin and urea synthesis rates for 10 days of perfusion were also demonstrated in the bioreactor with internal membrane oxygenator. These results are relevant in the design of hepatocyte bioreactors and the eventual scaling‐up to clinical devices. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 379–389, 2001.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>bioartificial liver</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell Survival</subject><subject>Coculture Techniques</subject><subject>Female</subject><subject>flat plate</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - metabolism</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Mice</subject><subject>microfabrication</subject><subject>Miscellaneous</subject><subject>Oxygen - metabolism</subject><subject>perfusion</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>shear stress</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotuCxC9APiDEJWA7iR0fYWlLpRVwKKrExZr4gzVk48V2aHPuH8dlI-CCuIxnNM-849GL0BNKXlJC2Kve55IIeg-tKJGiIkyS-2hFCOFV3Up2hI5T-lpK0XH-EB1RWjPSkm6Fbk-dszonHBwON_MXO0L2YcQwGuyGcI1LnrcW__DQ-8Hn-dCZRv0LK1MRMt7aPeSg52wT1kFPQ56iNdgXHbzzOga9hXG0Q5GEXO1LsLj3IVrQOcRH6IGDIdnHy3uCPp2dXq7fVZsP5xfr15tKt7SjlZFa9sCZIRSavm-0NLTcQI0A2ZDS4JzpThsOjjDo28ZxoJr1RjbgqHD1CXp-0N3H8H2yKaudT9oOA4w2TEkJIqSs6-a_YPlNI1rRFfDFASwnphStU_vodxBnRYm6c0YVZ9SdMwV9umhO_c6aP-BiRQGeLQAkDYOLMGqf_uIIqwUpWHXArv1g53_uU28uLpe9C-9Ttje_eYjfFBe1aNXV-3P18e3V2Waz_qzq-ic3m7WO</recordid><startdate>20010605</startdate><enddate>20010605</enddate><creator>Tilles, Arno W.</creator><creator>Baskaran, Harihara</creator><creator>Roy, Partha</creator><creator>Yarmush, Martin L.</creator><creator>Toner, Mehmet</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20010605</creationdate><title>Effects of oxygenation and flow on the viability and function of rat hepatocytes cocultured in a microchannel flat-plate bioreactor</title><author>Tilles, Arno W. ; Baskaran, Harihara ; Roy, Partha ; Yarmush, Martin L. ; Toner, Mehmet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5181-d9c9ba62d01a4bb4c9d15081d7a940a62662c8cd6af02ab54f6a1c2bd94af17f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>bioartificial liver</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Cell Survival</topic><topic>Coculture Techniques</topic><topic>Female</topic><topic>flat plate</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - metabolism</topic><topic>Industrial applications and implications. 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Bioeng</addtitle><date>2001-06-05</date><risdate>2001</risdate><volume>73</volume><issue>5</issue><spage>379</spage><epage>389</epage><pages>379-389</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The goal of this study was to investigate the viability and synthetic function of rat hepatocytes cocultured with 3T3‐J2 fibroblasts in a small‐scale microchannel flat‐plate bioreactor with and without an internal membrane oxygenator under flow. Bioreactor channel heights ranged between 85 and 500 μm and medium flow rates ranged between 0.06 and 4.18 mL/min. The results showed that the bioreactor without the oxygenator resulted in significantly decreased viability and function of hepatocytes, whereas hepatocytes in the bioreactor with internal membrane oxygenator were able to maintain their viability and function. The shear stress calculations showed that, at lower wall shear stresses (0.01 to 0.33 dyn/cm2), hepatocyte functions, measured as albumin and urea synthesis rates, were as much as 2.6‐ and 1.9‐fold greater, respectively, than those at higher wall shear stresses (5 to 21 dyn/cm2). Stable albumin and urea synthesis rates for 10 days of perfusion were also demonstrated in the bioreactor with internal membrane oxygenator. These results are relevant in the design of hepatocyte bioreactors and the eventual scaling‐up to clinical devices. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 379–389, 2001.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11320508</pmid><doi>10.1002/bit.1071</doi><tpages>11</tpages></addata></record> |
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| ispartof | Biotechnology and bioengineering, 2001-06, Vol.73 (5), p.379-389 |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | 3T3 Cells Animals bioartificial liver Biological and medical sciences Bioreactors Biotechnology Cell Survival Coculture Techniques Female flat plate Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Hepatocytes - cytology Hepatocytes - metabolism Industrial applications and implications. Economical aspects Mice microfabrication Miscellaneous Oxygen - metabolism perfusion Rats Rats, Inbred Lew shear stress |
| title | Effects of oxygenation and flow on the viability and function of rat hepatocytes cocultured in a microchannel flat-plate bioreactor |
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