Phosphate Stimulates Differentiation and Mineralization of the Chondroprogenitor Clone ATDC5
ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 μg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppresse...
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| Veröffentlicht in: | Japanese Journal of Pharmacology 2001, Vol.85(3), pp.278-281 |
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| container_title | Japanese Journal of Pharmacology |
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| creator | Fujita, Takashi Meguro, Tora Izumo, Nobuo Yasutomi, Chigusa Fukuyama, Ryo Nakamuta, Hiromichi Koida, Masao |
| description | ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 μg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 − 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis. |
| doi_str_mv | 10.1254/jjp.85.278 |
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The results suggest a regulatory role of Pi in endochondral osteogenesis.</description><identifier>ISSN: 0021-5198</identifier><identifier>EISSN: 1347-3506</identifier><identifier>DOI: 10.1254/jjp.85.278</identifier><identifier>PMID: 11325020</identifier><language>eng</language><publisher>Japan: The Japanese Pharmacological Society</publisher><subject>Animals ; ATDC5 ; Biomarkers - analysis ; Blotting, Northern ; Calcification, Physiologic - physiology ; Cell Differentiation ; Chondrocytes - cytology ; Chondrocytes - metabolism ; Clone Cells ; Collagen - metabolism ; Endochondral osteogenesis ; Mice ; Parathyroid Hormone - metabolism ; Parathyroid Hormone-Related Protein ; Phosphate ; Phosphates - metabolism ; Phosphates - pharmacology ; Proteins - metabolism ; Stem Cells - cytology ; Stem Cells - metabolism</subject><ispartof>The Japanese Journal of Pharmacology, 2001, Vol.85(3), pp.278-281</ispartof><rights>2001 Elsevier B.V.</rights><rights>The Japanese Pharmacological Society 2001</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c699t-c27a03347e7f0656d47428e68f86c1040195a84bb51d3969a113ccf8cd41d30e3</citedby><cites>FETCH-LOGICAL-c699t-c27a03347e7f0656d47428e68f86c1040195a84bb51d3969a113ccf8cd41d30e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11325020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujita, Takashi</creatorcontrib><creatorcontrib>Meguro, Tora</creatorcontrib><creatorcontrib>Izumo, Nobuo</creatorcontrib><creatorcontrib>Yasutomi, Chigusa</creatorcontrib><creatorcontrib>Fukuyama, Ryo</creatorcontrib><creatorcontrib>Nakamuta, Hiromichi</creatorcontrib><creatorcontrib>Koida, Masao</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><creatorcontrib>Faculty of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Setsunan University</creatorcontrib><title>Phosphate Stimulates Differentiation and Mineralization of the Chondroprogenitor Clone ATDC5</title><title>Japanese Journal of Pharmacology</title><addtitle>Jpn.J.Pharmacol.</addtitle><description>ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 μg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 − 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis.</description><subject>Animals</subject><subject>ATDC5</subject><subject>Biomarkers - analysis</subject><subject>Blotting, Northern</subject><subject>Calcification, Physiologic - physiology</subject><subject>Cell Differentiation</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - metabolism</subject><subject>Clone Cells</subject><subject>Collagen - metabolism</subject><subject>Endochondral osteogenesis</subject><subject>Mice</subject><subject>Parathyroid Hormone - metabolism</subject><subject>Parathyroid Hormone-Related Protein</subject><subject>Phosphate</subject><subject>Phosphates - metabolism</subject><subject>Phosphates - pharmacology</subject><subject>Proteins - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><issn>0021-5198</issn><issn>1347-3506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE9v1DAQxS0EokvhwgdAOXGolGWcxIlzQlVKAakIJMoNyfI6k8ZR1g62gwSfnqmyhQuX8Z95_vnNY-wlhz0vRPVmmpa9FPuikY_YjpdVk5cC6sdsB1DwXPBWnrFnMU50lMCrp-yM87IQUMCOff8y-riMOmH2NdnjOtMuZld2GDCgS1Yn612mXZ99sg6Dnu3v7coPWRox60bv-uCX4O_Q2eRD1s3eYXZ5e9WJ5-zJoOeIL07rOft2_e62-5DffH7_sbu8yU3dtik3RaOhJN_YDFCLuq-aqpBYy0HWhkMFvBVaVoeD4H3Z1q0m-8YM0vQVXQCW5-z1xiUbP1aMSR1tNDjP2qFfo2qgaZtGtCS82IQm-BgDDmoJ9qjDL8VB3WepKEslhaIsSfzqRF0PR-z_SU_hkeB6E1DXGk1zz5SRmvwaHI2rzFBPk19mVQBwBSAFlLS0CghPRfK6JBgn0NsNNMWk7_DvTzoka2Z8MFVu5f7xQ8eMOih0RKg2AlLMPy0GFY1FZ8hYQJNU7-3_RvwD8Juudg</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Fujita, Takashi</creator><creator>Meguro, Tora</creator><creator>Izumo, Nobuo</creator><creator>Yasutomi, Chigusa</creator><creator>Fukuyama, Ryo</creator><creator>Nakamuta, Hiromichi</creator><creator>Koida, Masao</creator><general>The Japanese Pharmacological Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>Phosphate Stimulates Differentiation and Mineralization of the Chondroprogenitor Clone ATDC5</title><author>Fujita, Takashi ; Meguro, Tora ; Izumo, Nobuo ; Yasutomi, Chigusa ; Fukuyama, Ryo ; Nakamuta, Hiromichi ; Koida, Masao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c699t-c27a03347e7f0656d47428e68f86c1040195a84bb51d3969a113ccf8cd41d30e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>ATDC5</topic><topic>Biomarkers - analysis</topic><topic>Blotting, Northern</topic><topic>Calcification, Physiologic - physiology</topic><topic>Cell Differentiation</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - metabolism</topic><topic>Clone Cells</topic><topic>Collagen - metabolism</topic><topic>Endochondral osteogenesis</topic><topic>Mice</topic><topic>Parathyroid Hormone - metabolism</topic><topic>Parathyroid Hormone-Related Protein</topic><topic>Phosphate</topic><topic>Phosphates - metabolism</topic><topic>Phosphates - pharmacology</topic><topic>Proteins - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Fujita, Takashi</creatorcontrib><creatorcontrib>Meguro, Tora</creatorcontrib><creatorcontrib>Izumo, Nobuo</creatorcontrib><creatorcontrib>Yasutomi, Chigusa</creatorcontrib><creatorcontrib>Fukuyama, Ryo</creatorcontrib><creatorcontrib>Nakamuta, Hiromichi</creatorcontrib><creatorcontrib>Koida, Masao</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><creatorcontrib>Faculty of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Setsunan University</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Journal of Pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujita, Takashi</au><au>Meguro, Tora</au><au>Izumo, Nobuo</au><au>Yasutomi, Chigusa</au><au>Fukuyama, Ryo</au><au>Nakamuta, Hiromichi</au><au>Koida, Masao</au><aucorp>Department of Pharmacology</aucorp><aucorp>Faculty of Pharmaceutical Sciences</aucorp><aucorp>Setsunan University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphate Stimulates Differentiation and Mineralization of the Chondroprogenitor Clone ATDC5</atitle><jtitle>Japanese Journal of Pharmacology</jtitle><addtitle>Jpn.J.Pharmacol.</addtitle><date>2001</date><risdate>2001</risdate><volume>85</volume><issue>3</issue><spage>278</spage><epage>281</epage><pages>278-281</pages><issn>0021-5198</issn><eissn>1347-3506</eissn><abstract>ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 μg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 − 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. 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| subjects | Animals ATDC5 Biomarkers - analysis Blotting, Northern Calcification, Physiologic - physiology Cell Differentiation Chondrocytes - cytology Chondrocytes - metabolism Clone Cells Collagen - metabolism Endochondral osteogenesis Mice Parathyroid Hormone - metabolism Parathyroid Hormone-Related Protein Phosphate Phosphates - metabolism Phosphates - pharmacology Proteins - metabolism Stem Cells - cytology Stem Cells - metabolism |
| title | Phosphate Stimulates Differentiation and Mineralization of the Chondroprogenitor Clone ATDC5 |
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