The Ligand Affinity of Proteins Measured by Isothermal Denaturation Kinetics

An isothermal denaturation kinetic method was developed for identifying potential ligands of proteins and measuring their affinity. The method is suitable for finding ligands specific toward proteins of unknown function and for large-scale drug screening. It consists of analyzing the kinetics of iso...

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Veröffentlicht in:	Analytical biochemistry 2001-05, Vol.292 (1), p.40-50
Hauptverfasser:	Epps, Dennis E., Sarver, Ronald W., Rogers, Joseph M., Herberg, John T., Tomich, Paul K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Circular Dichroism fluorescence isothermal denaturation Kinetics ligand binding Ligands Matrix Metalloproteinase 3 - analysis Nucleoside-Phosphate Kinase - analysis Protein Denaturation - physiology Spectrometry, Fluorescence - methods Temperature Time Factors UV absorbance
Online-Zugang:	Volltext
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