In vitro secretion of FSH by cultured clinically nonfunctioning and gonadotroph pituitary adenomas is directly correlated with locally produced levels of activin A
OBJECTIVE Expression of mRNAs encoding activin and its antagonists inhibin and follistatin has been described in human pituitary adenomas, including clinically nonfunctioning adenomas (NFAs) and gonadotroph adenomas (Gn‐omas). Since many of the NFAs and Gn‐omas secrete FSH in vitro, we hypothesized...
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| Veröffentlicht in: | Clinical endocrinology (Oxford) 2001-04, Vol.54 (4), p.485-492 |
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| container_title | Clinical endocrinology (Oxford) |
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| creator | Wessels, H. T. Hofland, L. J. Van Der Wal, R. Van Gastel, L. Van Koetsveld, P. M. De Herder, W. W. De Jong, F. H. |
| description | OBJECTIVE Expression of mRNAs encoding activin and its antagonists inhibin and follistatin has been described in human pituitary adenomas, including clinically nonfunctioning adenomas (NFAs) and gonadotroph adenomas (Gn‐omas). Since many of the NFAs and Gn‐omas secrete FSH in vitro, we hypothesized that locally produced activin may stimulate secretion of FSH in these pituitary adenomas.
PATIENTS and METHODS Pituitary adenoma tissue was obtained from 38 patients diagnosed preoperatively as having NFAs (n = 17), Gn‐omas (n = 5), prolactinomas (n = 6) or growth hormone (GH)‐producing adenomas (n = 10). Actual protein levels of activin, inhibin, follistatin, FSH and LH were measured in media of these 38 cultured pituitary adenomas. In addition, we investigated correlations between concentrations of these growth factors and hormones in NFAs and Gn‐omas.
RESULTS Gn‐omas were found to secrete significantly more activin A in their culture medium than PRL‐ and GH‐producing adenomas (P |
| doi_str_mv | 10.1046/j.1365-2265.2001.01194.x |
| format | Article |
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PATIENTS and METHODS Pituitary adenoma tissue was obtained from 38 patients diagnosed preoperatively as having NFAs (n = 17), Gn‐omas (n = 5), prolactinomas (n = 6) or growth hormone (GH)‐producing adenomas (n = 10). Actual protein levels of activin, inhibin, follistatin, FSH and LH were measured in media of these 38 cultured pituitary adenomas. In addition, we investigated correlations between concentrations of these growth factors and hormones in NFAs and Gn‐omas.
RESULTS Gn‐omas were found to secrete significantly more activin A in their culture medium than PRL‐ and GH‐producing adenomas (P < 0·05). Inhibin A and inhibin B protein levels in culture media were very low. A positive correlation between levels of activin A and FSH (r = 0·56, P < 0·005) was found, while no correlation between activin A and LH could be detected. Furthermore, levels of follistatin were positively correlated with activin A levels (r = 0·73, P < 0·0005). Comparison of the activin A:follistatin ratio with the measured FSH protein levels showed an even stronger relationship (r = 0·79, P < 0·0005).
CONCLUSIONS It is concluded that levels of activin A, follistatin and FSH in media of cultured nonfunctioning adenomas and gonadotroph adenomas are positively correlated. This suggests that these adenomas secrete FSH in response to the relatively high locally produced levels of activin A.</description><identifier>ISSN: 0300-0664</identifier><identifier>EISSN: 1365-2265</identifier><identifier>DOI: 10.1046/j.1365-2265.2001.01194.x</identifier><identifier>PMID: 11318784</identifier><identifier>CODEN: CLECAP</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Activins - analysis ; Activins - metabolism ; Adenoma - metabolism ; Adult ; Aged ; Analysis of Variance ; Biological and medical sciences ; Culture Media - chemistry ; Endocrinopathies ; Female ; Follicle Stimulating Hormone - analysis ; Follicle Stimulating Hormone - metabolism ; Follistatin ; Humans ; Hypothalamus. Hypophysis. Epiphysis (diseases) ; Inhibin-beta Subunits - analysis ; Inhibin-beta Subunits - metabolism ; Inhibins - analysis ; Luteinizing Hormone - analysis ; Male ; Medical sciences ; Middle Aged ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Pituitary Neoplasms - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Cells, Cultured</subject><ispartof>Clinical endocrinology (Oxford), 2001-04, Vol.54 (4), p.485-492</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Apr 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5084-1c9ed0261dcbda7f16da624b0d782f193903256b809de8cf51c4edae7a07d0db3</citedby><cites>FETCH-LOGICAL-c5084-1c9ed0261dcbda7f16da624b0d782f193903256b809de8cf51c4edae7a07d0db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2265.2001.01194.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2265.2001.01194.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=964326$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11318784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wessels, H. T.</creatorcontrib><creatorcontrib>Hofland, L. J.</creatorcontrib><creatorcontrib>Van Der Wal, R.</creatorcontrib><creatorcontrib>Van Gastel, L.</creatorcontrib><creatorcontrib>Van Koetsveld, P. M.</creatorcontrib><creatorcontrib>De Herder, W. W.</creatorcontrib><creatorcontrib>De Jong, F. H.</creatorcontrib><title>In vitro secretion of FSH by cultured clinically nonfunctioning and gonadotroph pituitary adenomas is directly correlated with locally produced levels of activin A</title><title>Clinical endocrinology (Oxford)</title><addtitle>Clin Endocrinol (Oxf)</addtitle><description>OBJECTIVE Expression of mRNAs encoding activin and its antagonists inhibin and follistatin has been described in human pituitary adenomas, including clinically nonfunctioning adenomas (NFAs) and gonadotroph adenomas (Gn‐omas). Since many of the NFAs and Gn‐omas secrete FSH in vitro, we hypothesized that locally produced activin may stimulate secretion of FSH in these pituitary adenomas.
PATIENTS and METHODS Pituitary adenoma tissue was obtained from 38 patients diagnosed preoperatively as having NFAs (n = 17), Gn‐omas (n = 5), prolactinomas (n = 6) or growth hormone (GH)‐producing adenomas (n = 10). Actual protein levels of activin, inhibin, follistatin, FSH and LH were measured in media of these 38 cultured pituitary adenomas. In addition, we investigated correlations between concentrations of these growth factors and hormones in NFAs and Gn‐omas.
RESULTS Gn‐omas were found to secrete significantly more activin A in their culture medium than PRL‐ and GH‐producing adenomas (P < 0·05). Inhibin A and inhibin B protein levels in culture media were very low. A positive correlation between levels of activin A and FSH (r = 0·56, P < 0·005) was found, while no correlation between activin A and LH could be detected. Furthermore, levels of follistatin were positively correlated with activin A levels (r = 0·73, P < 0·0005). Comparison of the activin A:follistatin ratio with the measured FSH protein levels showed an even stronger relationship (r = 0·79, P < 0·0005).
CONCLUSIONS It is concluded that levels of activin A, follistatin and FSH in media of cultured nonfunctioning adenomas and gonadotroph adenomas are positively correlated. This suggests that these adenomas secrete FSH in response to the relatively high locally produced levels of activin A.</description><subject>Activins - analysis</subject><subject>Activins - metabolism</subject><subject>Adenoma - metabolism</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis of Variance</subject><subject>Biological and medical sciences</subject><subject>Culture Media - chemistry</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - analysis</subject><subject>Follicle Stimulating Hormone - metabolism</subject><subject>Follistatin</subject><subject>Humans</subject><subject>Hypothalamus. Hypophysis. Epiphysis (diseases)</subject><subject>Inhibin-beta Subunits - analysis</subject><subject>Inhibin-beta Subunits - metabolism</subject><subject>Inhibins - analysis</subject><subject>Luteinizing Hormone - analysis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Pituitary Neoplasms - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Tumor Cells, Cultured</subject><issn>0300-0664</issn><issn>1365-2265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEUhS0EoqHwCsgCid0M1_NjzyxYlKhNq1Zl0SKWlsf2tA6OHeyZNHkeXhRPEwWJFStb9nfOPboHIUwgJ1DRz8uclLTOioLWeQFAciCkrfLtCzQ7frxEMygBMqC0OkFvYlwCQN0Ae41OCClJw5pqhn5fObwxQ_A4ahn0YLzDvscXd5e422E52mEMWmFpjTNSWLvDzrt-dHIijXvAwin84J1QPpmsH_HaDKMZRNhhobTzKxGxiViZoOWQ1NKHoK0YkueTGR6x9XvXdfBqlOnV6o22ccog0oyNcfjsLXrVCxv1u8N5ir5fnN_PL7Obb4ur-dlNJmtoqozIVisoKFGyU4L1hCpBi6oDxZqiJ23ZQlnUtGugVbqRfU1kpZXQTABToLryFH3a-6Ywv0YdB74yUWprhdN-jJwBSyZ1kcAP_4BLPwaXsnHSNrQhLasS1OwhGXyMQfd8HcwqLYYT4FOLfMmnsvhUFp9a5M8t8m2Svj_4j91Kq7_CQ20J-HgAREz764Nw0sQj19KqLGiivuypJ2P17r_H8_n57XRL-myvN3HQ26NehJ-cspLV_Mftgi_ar9cLVt_x-_IPbrfKkw</recordid><startdate>200104</startdate><enddate>200104</enddate><creator>Wessels, H. T.</creator><creator>Hofland, L. J.</creator><creator>Van Der Wal, R.</creator><creator>Van Gastel, L.</creator><creator>Van Koetsveld, P. M.</creator><creator>De Herder, W. W.</creator><creator>De Jong, F. H.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>200104</creationdate><title>In vitro secretion of FSH by cultured clinically nonfunctioning and gonadotroph pituitary adenomas is directly correlated with locally produced levels of activin A</title><author>Wessels, H. T. ; Hofland, L. J. ; Van Der Wal, R. ; Van Gastel, L. ; Van Koetsveld, P. M. ; De Herder, W. W. ; De Jong, F. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5084-1c9ed0261dcbda7f16da624b0d782f193903256b809de8cf51c4edae7a07d0db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Activins - analysis</topic><topic>Activins - metabolism</topic><topic>Adenoma - metabolism</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis of Variance</topic><topic>Biological and medical sciences</topic><topic>Culture Media - chemistry</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - analysis</topic><topic>Follicle Stimulating Hormone - metabolism</topic><topic>Follistatin</topic><topic>Humans</topic><topic>Hypothalamus. Hypophysis. Epiphysis (diseases)</topic><topic>Inhibin-beta Subunits - analysis</topic><topic>Inhibin-beta Subunits - metabolism</topic><topic>Inhibins - analysis</topic><topic>Luteinizing Hormone - analysis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Pituitary Neoplasms - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wessels, H. T.</creatorcontrib><creatorcontrib>Hofland, L. J.</creatorcontrib><creatorcontrib>Van Der Wal, R.</creatorcontrib><creatorcontrib>Van Gastel, L.</creatorcontrib><creatorcontrib>Van Koetsveld, P. M.</creatorcontrib><creatorcontrib>De Herder, W. W.</creatorcontrib><creatorcontrib>De Jong, F. H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical endocrinology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wessels, H. T.</au><au>Hofland, L. J.</au><au>Van Der Wal, R.</au><au>Van Gastel, L.</au><au>Van Koetsveld, P. M.</au><au>De Herder, W. W.</au><au>De Jong, F. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro secretion of FSH by cultured clinically nonfunctioning and gonadotroph pituitary adenomas is directly correlated with locally produced levels of activin A</atitle><jtitle>Clinical endocrinology (Oxford)</jtitle><addtitle>Clin Endocrinol (Oxf)</addtitle><date>2001-04</date><risdate>2001</risdate><volume>54</volume><issue>4</issue><spage>485</spage><epage>492</epage><pages>485-492</pages><issn>0300-0664</issn><eissn>1365-2265</eissn><coden>CLECAP</coden><abstract>OBJECTIVE Expression of mRNAs encoding activin and its antagonists inhibin and follistatin has been described in human pituitary adenomas, including clinically nonfunctioning adenomas (NFAs) and gonadotroph adenomas (Gn‐omas). Since many of the NFAs and Gn‐omas secrete FSH in vitro, we hypothesized that locally produced activin may stimulate secretion of FSH in these pituitary adenomas.
PATIENTS and METHODS Pituitary adenoma tissue was obtained from 38 patients diagnosed preoperatively as having NFAs (n = 17), Gn‐omas (n = 5), prolactinomas (n = 6) or growth hormone (GH)‐producing adenomas (n = 10). Actual protein levels of activin, inhibin, follistatin, FSH and LH were measured in media of these 38 cultured pituitary adenomas. In addition, we investigated correlations between concentrations of these growth factors and hormones in NFAs and Gn‐omas.
RESULTS Gn‐omas were found to secrete significantly more activin A in their culture medium than PRL‐ and GH‐producing adenomas (P < 0·05). Inhibin A and inhibin B protein levels in culture media were very low. A positive correlation between levels of activin A and FSH (r = 0·56, P < 0·005) was found, while no correlation between activin A and LH could be detected. Furthermore, levels of follistatin were positively correlated with activin A levels (r = 0·73, P < 0·0005). Comparison of the activin A:follistatin ratio with the measured FSH protein levels showed an even stronger relationship (r = 0·79, P < 0·0005).
CONCLUSIONS It is concluded that levels of activin A, follistatin and FSH in media of cultured nonfunctioning adenomas and gonadotroph adenomas are positively correlated. This suggests that these adenomas secrete FSH in response to the relatively high locally produced levels of activin A.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11318784</pmid><doi>10.1046/j.1365-2265.2001.01194.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Activins - analysis Activins - metabolism Adenoma - metabolism Adult Aged Analysis of Variance Biological and medical sciences Culture Media - chemistry Endocrinopathies Female Follicle Stimulating Hormone - analysis Follicle Stimulating Hormone - metabolism Follistatin Humans Hypothalamus. Hypophysis. Epiphysis (diseases) Inhibin-beta Subunits - analysis Inhibin-beta Subunits - metabolism Inhibins - analysis Luteinizing Hormone - analysis Male Medical sciences Middle Aged Non tumoral diseases. Target tissue resistance. Benign neoplasms Pituitary Neoplasms - metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured |
| title | In vitro secretion of FSH by cultured clinically nonfunctioning and gonadotroph pituitary adenomas is directly correlated with locally produced levels of activin A |
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