Comparison of a commercial qualitative real-time RT-PCR kit with direct immunofluorescence assay (DFA) and cell culture for detection of influenza A and B in children

Abstract Background Institutional pandemic planning prompted a study of the molecular detection of influenza virus from respiratory specimens in children, compared to conventional diagnostics. Objective To evaluate the performance of a commercial qualitative real-time RT-PCR kit (rRT-PCR), the artus...

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Veröffentlicht in:	Journal of clinical virology 2008-06, Vol.42 (2), p.190-193
Hauptverfasser:	Gharabaghi, Farhad, Tellier, Raymond, Cheung, Rose, Collins, Carol, Broukhanski, George, Drews, Steven J, Richardson, Susan E
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Allergy and Immunology artus™ rRT-PCR Biological and medical sciences Child Child, Preschool Children Culture DFA Fluorescent Antibody Technique, Direct Fundamental and applied biological sciences. Psychology Human viral diseases Humans Infant Infant, Newborn Infectious Disease Infectious diseases Influenza Influenza A virus - genetics Influenza A virus - immunology Influenza A virus - isolation & purification Influenza B virus - genetics Influenza B virus - immunology Influenza B virus - isolation & purification Influenza virus Influenza, Human - diagnosis Influenza, Human - virology Medical sciences Microbiology Miscellaneous Nose - virology Reagent Kits, Diagnostic Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - analysis Sensitivity and Specificity Specimen Handling - methods Viral diseases Virology Virus Cultivation
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