PCR-based identification of individuals of Schistosoma japonicum representing different subpopulations using a genetic marker in mitochondrial DNA
A mitochondrial NADH dehydrogenase I gene fragment (NDI) was sequenced for three laboratory maintained isolates of Schistosoma japonicum. Comparison of sequences representing the isolates (originally obtained from the Anhui and Zhejiang provinces of the People's Republic of China, and from the...
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| Veröffentlicht in: | International journal for parasitology 1999-07, Vol.29 (7), p.1121-1128 |
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| description | A mitochondrial NADH dehydrogenase I gene fragment (NDI) was sequenced for three laboratory maintained isolates of
Schistosoma japonicum. Comparison of sequences representing the isolates (originally obtained from the Anhui and Zhejiang provinces of the People's Republic of China, and from the Philippines) revealed inter-isolate sequence variations of 0.2–0.6% and no intra-isolate variation was found. The sequences also indicated that while the amplification products of the Zhejiang and Philippine isolates contained a recognition site for the endonuclease
RsaI, there was no such site in the Anhui isolate. This was tested by digesting amplification products from a number of individual worms with
RsaI. Then an infection experiment was designed to test the value of this genetic marker for studies of the population biology of
S. japonicum in the final host. For this, the two Chinese isolates were used. Three groups of mice (A–C) were exposed firstly to a primary infection and then challenge-infected at weeks 4 and 7 of the experiment. In group A the first infection was done with the Anhui isolate, and the two others with the Zhejiang isolate, thereby providing a specific, detectable cohort. In groups B and C the Anhui isolate was used for the second and third infection. All mice were perfused 5 weeks after the last challenge infection, and the NDI was subsequently amplified from DNA of the perfused worms and digested with
RsaI. The digestion revealed that while infection groups A and B contained mixed populations of the Anhui and Zhejiang isolates, only Zhejiang worms were present in group C. We concluded that the absence/presence of the
RsaI site in the NDI provides a useful marker for the delineation of cohorts of
S. japonicum. |
| doi_str_mv | 10.1016/S0020-7519(99)00040-5 |
| format | Article |
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Schistosoma japonicum. Comparison of sequences representing the isolates (originally obtained from the Anhui and Zhejiang provinces of the People's Republic of China, and from the Philippines) revealed inter-isolate sequence variations of 0.2–0.6% and no intra-isolate variation was found. The sequences also indicated that while the amplification products of the Zhejiang and Philippine isolates contained a recognition site for the endonuclease
RsaI, there was no such site in the Anhui isolate. This was tested by digesting amplification products from a number of individual worms with
RsaI. Then an infection experiment was designed to test the value of this genetic marker for studies of the population biology of
S. japonicum in the final host. For this, the two Chinese isolates were used. Three groups of mice (A–C) were exposed firstly to a primary infection and then challenge-infected at weeks 4 and 7 of the experiment. In group A the first infection was done with the Anhui isolate, and the two others with the Zhejiang isolate, thereby providing a specific, detectable cohort. In groups B and C the Anhui isolate was used for the second and third infection. All mice were perfused 5 weeks after the last challenge infection, and the NDI was subsequently amplified from DNA of the perfused worms and digested with
RsaI. The digestion revealed that while infection groups A and B contained mixed populations of the Anhui and Zhejiang isolates, only Zhejiang worms were present in group C. We concluded that the absence/presence of the
RsaI site in the NDI provides a useful marker for the delineation of cohorts of
S. japonicum.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/S0020-7519(99)00040-5</identifier><identifier>PMID: 10501622</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Deoxyribonucleases, Type II Site-Specific - metabolism ; DNA, Helminth - genetics ; DNA, Mitochondrial - genetics ; Electron Transport Complex I ; Female ; Fundamental and applied biological sciences. Psychology ; Genetic Markers ; Genetic Variation ; Genetics of eukaryotes. Biological and molecular evolution ; Invertebrata ; Male ; Mating behaviour ; Mice ; Mitochondria - enzymology ; Molecular Sequence Data ; NADH dehydrogenase I (NDI) ; NADH, NADPH Oxidoreductases - genetics ; Parasite Egg Count ; PCR–RFLP ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; Population biology ; Population genetics, reproduction patterns ; Schistosoma japonicum ; Schistosoma japonicum - classification ; Schistosoma japonicum - genetics ; Schistosoma japonicum - physiology ; Schistosomiasis japonica - parasitology ; Sequence Analysis, DNA ; specific cohorts</subject><ispartof>International journal for parasitology, 1999-07, Vol.29 (7), p.1121-1128</ispartof><rights>1999 Australian Society for Parasitology Inc</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0020-7519(99)00040-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1931821$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10501622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sørensen, E</creatorcontrib><creatorcontrib>Bøgh, H.O</creatorcontrib><creatorcontrib>Johansen, M.V</creatorcontrib><creatorcontrib>McManus, D.P</creatorcontrib><title>PCR-based identification of individuals of Schistosoma japonicum representing different subpopulations using a genetic marker in mitochondrial DNA</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>A mitochondrial NADH dehydrogenase I gene fragment (NDI) was sequenced for three laboratory maintained isolates of
Schistosoma japonicum. Comparison of sequences representing the isolates (originally obtained from the Anhui and Zhejiang provinces of the People's Republic of China, and from the Philippines) revealed inter-isolate sequence variations of 0.2–0.6% and no intra-isolate variation was found. The sequences also indicated that while the amplification products of the Zhejiang and Philippine isolates contained a recognition site for the endonuclease
RsaI, there was no such site in the Anhui isolate. This was tested by digesting amplification products from a number of individual worms with
RsaI. Then an infection experiment was designed to test the value of this genetic marker for studies of the population biology of
S. japonicum in the final host. For this, the two Chinese isolates were used. Three groups of mice (A–C) were exposed firstly to a primary infection and then challenge-infected at weeks 4 and 7 of the experiment. In group A the first infection was done with the Anhui isolate, and the two others with the Zhejiang isolate, thereby providing a specific, detectable cohort. In groups B and C the Anhui isolate was used for the second and third infection. All mice were perfused 5 weeks after the last challenge infection, and the NDI was subsequently amplified from DNA of the perfused worms and digested with
RsaI. The digestion revealed that while infection groups A and B contained mixed populations of the Anhui and Zhejiang isolates, only Zhejiang worms were present in group C. We concluded that the absence/presence of the
RsaI site in the NDI provides a useful marker for the delineation of cohorts of
S. japonicum.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleases, Type II Site-Specific - metabolism</subject><subject>DNA, Helminth - genetics</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Electron Transport Complex I</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Markers</subject><subject>Genetic Variation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Invertebrata</subject><subject>Male</subject><subject>Mating behaviour</subject><subject>Mice</subject><subject>Mitochondria - enzymology</subject><subject>Molecular Sequence Data</subject><subject>NADH dehydrogenase I (NDI)</subject><subject>NADH, NADPH Oxidoreductases - genetics</subject><subject>Parasite Egg Count</subject><subject>PCR–RFLP</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Population biology</subject><subject>Population genetics, reproduction patterns</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma japonicum - classification</subject><subject>Schistosoma japonicum - genetics</subject><subject>Schistosoma japonicum - physiology</subject><subject>Schistosomiasis japonica - parasitology</subject><subject>Sequence Analysis, DNA</subject><subject>specific cohorts</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkt9uFSEQxonR2GP1ETRcGKMXq7AsZblqmuPfpFFj9ZpwYGin7sIKu018DZ9Y9vSol3JDJvPj-zLDR8hjzl5yxk9eXTDWskZJrp9r_YIx1rFG3iEb3ivdMC7kXbL5ixyRB6VcM8al6Lr75IgzWTXadkN-fd5-aXa2gKfoIc4Y0NkZU6QpUIweb9AvdihreeGusMyppNHSazuliG4ZaYYpQ1mfxkvqMQTItaBl2U1pWoa9WKFLWduWXkKEGR0dbf4OuTrQEefkrlL0Ge1AX388e0juheoIjw73Mfn29s3X7fvm_NO7D9uz8wZEK-ZGe2t171TnwQPvufDedk4x0QbNZRAqgOD1nEDnmbM755y1QXaas532Sopj8uxWd8rpxwJlNiMWB8NgI6SlGMVUr4Ts_wtyJdpW8a6CTw7gshvBmyljnfOn-bPuCjw9ALY4O4Rso8Pyj9OC9y2v2OktBnX8G4RsikOIDjxmcLPxCaumWXNg9jkw6ycbrc0-B0aK32Jnp0s</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Sørensen, E</creator><creator>Bøgh, H.O</creator><creator>Johansen, M.V</creator><creator>McManus, D.P</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990701</creationdate><title>PCR-based identification of individuals of Schistosoma japonicum representing different subpopulations using a genetic marker in mitochondrial DNA</title><author>Sørensen, E ; Bøgh, H.O ; Johansen, M.V ; McManus, D.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e323t-9daa98c74dede1813dda4c7032f915f37fe311116e4d0cabcccaaf54910b9d753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleases, Type II Site-Specific - metabolism</topic><topic>DNA, Helminth - genetics</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Electron Transport Complex I</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Markers</topic><topic>Genetic Variation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Invertebrata</topic><topic>Male</topic><topic>Mating behaviour</topic><topic>Mice</topic><topic>Mitochondria - enzymology</topic><topic>Molecular Sequence Data</topic><topic>NADH dehydrogenase I (NDI)</topic><topic>NADH, NADPH Oxidoreductases - genetics</topic><topic>Parasite Egg Count</topic><topic>PCR–RFLP</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Population biology</topic><topic>Population genetics, reproduction patterns</topic><topic>Schistosoma japonicum</topic><topic>Schistosoma japonicum - classification</topic><topic>Schistosoma japonicum - genetics</topic><topic>Schistosoma japonicum - physiology</topic><topic>Schistosomiasis japonica - parasitology</topic><topic>Sequence Analysis, DNA</topic><topic>specific cohorts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sørensen, E</creatorcontrib><creatorcontrib>Bøgh, H.O</creatorcontrib><creatorcontrib>Johansen, M.V</creatorcontrib><creatorcontrib>McManus, D.P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sørensen, E</au><au>Bøgh, H.O</au><au>Johansen, M.V</au><au>McManus, D.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR-based identification of individuals of Schistosoma japonicum representing different subpopulations using a genetic marker in mitochondrial DNA</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>29</volume><issue>7</issue><spage>1121</spage><epage>1128</epage><pages>1121-1128</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>A mitochondrial NADH dehydrogenase I gene fragment (NDI) was sequenced for three laboratory maintained isolates of
Schistosoma japonicum. Comparison of sequences representing the isolates (originally obtained from the Anhui and Zhejiang provinces of the People's Republic of China, and from the Philippines) revealed inter-isolate sequence variations of 0.2–0.6% and no intra-isolate variation was found. The sequences also indicated that while the amplification products of the Zhejiang and Philippine isolates contained a recognition site for the endonuclease
RsaI, there was no such site in the Anhui isolate. This was tested by digesting amplification products from a number of individual worms with
RsaI. Then an infection experiment was designed to test the value of this genetic marker for studies of the population biology of
S. japonicum in the final host. For this, the two Chinese isolates were used. Three groups of mice (A–C) were exposed firstly to a primary infection and then challenge-infected at weeks 4 and 7 of the experiment. In group A the first infection was done with the Anhui isolate, and the two others with the Zhejiang isolate, thereby providing a specific, detectable cohort. In groups B and C the Anhui isolate was used for the second and third infection. All mice were perfused 5 weeks after the last challenge infection, and the NDI was subsequently amplified from DNA of the perfused worms and digested with
RsaI. The digestion revealed that while infection groups A and B contained mixed populations of the Anhui and Zhejiang isolates, only Zhejiang worms were present in group C. We concluded that the absence/presence of the
RsaI site in the NDI provides a useful marker for the delineation of cohorts of
S. japonicum.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10501622</pmid><doi>10.1016/S0020-7519(99)00040-5</doi><tpages>8</tpages></addata></record> |
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| ispartof | International journal for parasitology, 1999-07, Vol.29 (7), p.1121-1128 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Deoxyribonucleases, Type II Site-Specific - metabolism DNA, Helminth - genetics DNA, Mitochondrial - genetics Electron Transport Complex I Female Fundamental and applied biological sciences. Psychology Genetic Markers Genetic Variation Genetics of eukaryotes. Biological and molecular evolution Invertebrata Male Mating behaviour Mice Mitochondria - enzymology Molecular Sequence Data NADH dehydrogenase I (NDI) NADH, NADPH Oxidoreductases - genetics Parasite Egg Count PCR–RFLP Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Population biology Population genetics, reproduction patterns Schistosoma japonicum Schistosoma japonicum - classification Schistosoma japonicum - genetics Schistosoma japonicum - physiology Schistosomiasis japonica - parasitology Sequence Analysis, DNA specific cohorts |
| title | PCR-based identification of individuals of Schistosoma japonicum representing different subpopulations using a genetic marker in mitochondrial DNA |
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