The Dynamics of Structural Deformations of Immobilized Single Light-Harvesting Complexes
Single assemblies of the intact light-harvesting complex LH2 from Rhodopseudomonas acidophila were bound to mica surfaces at 300 K and examined by observing their fluorescence after polarized light excitation. The complexes are generally not cylindrically symmetric. They act like elliptic absorbers,...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-09, Vol.96 (20), p.11271-11276 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Bopp, M A Sytnik, A Howard, T D Cogdell, R J Hochstrasser, R M |
| description | Single assemblies of the intact light-harvesting complex LH2 from Rhodopseudomonas acidophila were bound to mica surfaces at 300 K and examined by observing their fluorescence after polarized light excitation. The complexes are generally not cylindrically symmetric. They act like elliptic absorbers, indicating that the high symmetry found in crystals of LH2 is not present when the molecules are immobilized on mica. The ellipticity and the principal axes of the ellipses fluctuate on the time scale of seconds, indicating that there is a mobile structural deformation. The B850 ring of cofactors shows significantly less asymmetry than B800. The photobleaching strongly depends on the presence of oxygen. |
| doi_str_mv | 10.1073/pnas.96.20.11271 |
| format | Article |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1999-09, Vol.96 (20), p.11271-11276 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biological Sciences Biophysics Ellipses Ellipticity Fluorescence Lasers Linear polarization Mica Molecules Photosynthetic Reaction Center Complex Proteins - chemistry Polarized light Rhodopseudomonas - chemistry Rhodopseudomonas acidophila Trajectories |
| title | The Dynamics of Structural Deformations of Immobilized Single Light-Harvesting Complexes |
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