Design of a Modular Bioreactor to Incorporate Both Perfusion Flow and Hydrostatic Compression for Tissue Engineering Applications
Physiological models have demonstrated that cells undergo a cyclic regimen of hydrostatic compression and fluid shear stress within the lacunar-canalicular porosity of bone. A new modular bioreactor was designed to incorporate both perfusion fluid flow and hydrostatic compression in an effort to mor...
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| Veröffentlicht in: | Annals of biomedical engineering 2008-07, Vol.36 (7), p.1228-1241 |
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| container_title | Annals of biomedical engineering |
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| creator | Orr, David E. Burg, Karen J. L. |
| description | Physiological models have demonstrated that cells undergo a cyclic regimen of hydrostatic compression and fluid shear stress within the lacunar-canalicular porosity of bone. A new modular bioreactor was designed to incorporate both perfusion fluid flow and hydrostatic compression in an effort to more accurately simulate the mechanical loading and stress found in natural bone
in vivo
. The bioreactor design incorporated custom and off-the-shelf components to produce levels of mechanical stimuli relevant to the physiologic range, including hydrostatic compression exceeding 300 kPa and perfusion shear stress of 0.7 dyne/cm
2
. Preliminary findings indicated that the novel system facilitated the viable growth of cells on discrete tissue engineering scaffolds. The bioreactor has established an experimental platform for ongoing investigation of the interactive effect of perfusion fluid flow and hydrostatic compression on multiple cell types. |
| doi_str_mv | 10.1007/s10439-008-9505-0 |
| format | Article |
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in vivo
. The bioreactor design incorporated custom and off-the-shelf components to produce levels of mechanical stimuli relevant to the physiologic range, including hydrostatic compression exceeding 300 kPa and perfusion shear stress of 0.7 dyne/cm
2
. Preliminary findings indicated that the novel system facilitated the viable growth of cells on discrete tissue engineering scaffolds. The bioreactor has established an experimental platform for ongoing investigation of the interactive effect of perfusion fluid flow and hydrostatic compression on multiple cell types.</description><identifier>ISSN: 0090-6964</identifier><identifier>EISSN: 1573-9686</identifier><identifier>DOI: 10.1007/s10439-008-9505-0</identifier><identifier>PMID: 18438713</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>3T3 Cells ; Animals ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Biophysics ; Bioreactors ; Cell Culture Techniques - instrumentation ; Cell Culture Techniques - methods ; Cell Proliferation ; Cell Survival - physiology ; Classical Mechanics ; Compressive Strength ; Computer-Aided Design ; Fluid flow ; Mechanotransduction, Cellular - physiology ; Mice ; Microfluidics - instrumentation ; Microfluidics - methods ; Perfusion - instrumentation ; Porosity ; Shear stress ; Tissue Engineering - instrumentation ; Tissue Engineering - methods</subject><ispartof>Annals of biomedical engineering, 2008-07, Vol.36 (7), p.1228-1241</ispartof><rights>Biomedical Engineering Society 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-6fc4cde89079feb6d520a10d34f6eacc2feca1852672e13a6699793f1e4569d13</citedby><cites>FETCH-LOGICAL-c466t-6fc4cde89079feb6d520a10d34f6eacc2feca1852672e13a6699793f1e4569d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10439-008-9505-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10439-008-9505-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18438713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Orr, David E.</creatorcontrib><creatorcontrib>Burg, Karen J. L.</creatorcontrib><title>Design of a Modular Bioreactor to Incorporate Both Perfusion Flow and Hydrostatic Compression for Tissue Engineering Applications</title><title>Annals of biomedical engineering</title><addtitle>Ann Biomed Eng</addtitle><addtitle>Ann Biomed Eng</addtitle><description>Physiological models have demonstrated that cells undergo a cyclic regimen of hydrostatic compression and fluid shear stress within the lacunar-canalicular porosity of bone. A new modular bioreactor was designed to incorporate both perfusion fluid flow and hydrostatic compression in an effort to more accurately simulate the mechanical loading and stress found in natural bone
in vivo
. The bioreactor design incorporated custom and off-the-shelf components to produce levels of mechanical stimuli relevant to the physiologic range, including hydrostatic compression exceeding 300 kPa and perfusion shear stress of 0.7 dyne/cm
2
. Preliminary findings indicated that the novel system facilitated the viable growth of cells on discrete tissue engineering scaffolds. The bioreactor has established an experimental platform for ongoing investigation of the interactive effect of perfusion fluid flow and hydrostatic compression on multiple cell types.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Bioreactors</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Proliferation</subject><subject>Cell Survival - physiology</subject><subject>Classical Mechanics</subject><subject>Compressive Strength</subject><subject>Computer-Aided Design</subject><subject>Fluid flow</subject><subject>Mechanotransduction, Cellular - physiology</subject><subject>Mice</subject><subject>Microfluidics - instrumentation</subject><subject>Microfluidics - methods</subject><subject>Perfusion - instrumentation</subject><subject>Porosity</subject><subject>Shear stress</subject><subject>Tissue Engineering - instrumentation</subject><subject>Tissue Engineering - methods</subject><issn>0090-6964</issn><issn>1573-9686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1v1DAQhi0EokvhB3BBFgdugXGc-OPYLi2tVASHcrZcZ7y4ytrBToR65J_jsCtVQkKcfHn8zMz7EvKawXsGID8UBh3XDYBqdA99A0_IhvWSN1oo8ZRsADQ0QovuhLwo5R6AMcX75-SEqY4ryfiG_PqIJewiTZ5a-jkNy2gzPQ8po3VzynRO9Dq6lKeU7Yz0PM3f6VfMfikhRXo5pp_UxoFePQw5ldnOwdFt2k8Zyx_AV8VtKGVBehF3ISLmEHf0bJrG4CqdYnlJnnk7Fnx1fE_Jt8uL2-1Vc_Pl0_X27KZxnRBzI7zr3IBKg9Qe78TQt2AZDLzzou7qWo_OMtW3QrbIuBVCa6m5Z9j1Qg-Mn5J3B--U048Fy2z2oTgcRxsxLcVIkKqrwf0X5LwVopdQwbd_gfdpybEeYVpQWrZMrjZ2gFwNqGT0Zsphb_ODYWDWFs2hRVNbNGuLZhW_OYqXuz0Ojz-OtVWgPQBlWvPE_Dj539bfXAapBw</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Orr, David E.</creator><creator>Burg, Karen J. 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L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Design of a Modular Bioreactor to Incorporate Both Perfusion Flow and Hydrostatic Compression for Tissue Engineering Applications</atitle><jtitle>Annals of biomedical engineering</jtitle><stitle>Ann Biomed Eng</stitle><addtitle>Ann Biomed Eng</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>36</volume><issue>7</issue><spage>1228</spage><epage>1241</epage><pages>1228-1241</pages><issn>0090-6964</issn><eissn>1573-9686</eissn><abstract>Physiological models have demonstrated that cells undergo a cyclic regimen of hydrostatic compression and fluid shear stress within the lacunar-canalicular porosity of bone. A new modular bioreactor was designed to incorporate both perfusion fluid flow and hydrostatic compression in an effort to more accurately simulate the mechanical loading and stress found in natural bone
in vivo
. The bioreactor design incorporated custom and off-the-shelf components to produce levels of mechanical stimuli relevant to the physiologic range, including hydrostatic compression exceeding 300 kPa and perfusion shear stress of 0.7 dyne/cm
2
. Preliminary findings indicated that the novel system facilitated the viable growth of cells on discrete tissue engineering scaffolds. The bioreactor has established an experimental platform for ongoing investigation of the interactive effect of perfusion fluid flow and hydrostatic compression on multiple cell types.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>18438713</pmid><doi>10.1007/s10439-008-9505-0</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-6964 |
| ispartof | Annals of biomedical engineering, 2008-07, Vol.36 (7), p.1228-1241 |
| issn | 0090-6964 1573-9686 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | 3T3 Cells Animals Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Biophysics Bioreactors Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Proliferation Cell Survival - physiology Classical Mechanics Compressive Strength Computer-Aided Design Fluid flow Mechanotransduction, Cellular - physiology Mice Microfluidics - instrumentation Microfluidics - methods Perfusion - instrumentation Porosity Shear stress Tissue Engineering - instrumentation Tissue Engineering - methods |
| title | Design of a Modular Bioreactor to Incorporate Both Perfusion Flow and Hydrostatic Compression for Tissue Engineering Applications |
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