Characterization of estrogen receptor-beta (ERbeta) messenger ribonucleic acid and protein expression in rat granulosa cells

We have examined estrogen-responsiveness of ovarian granulosa cells by focusing on estrogen receptor (ER) expression. Estrogen responsiveness was determined by examining the effect of 17beta-estradiol (1-10 nM) on luciferase reporter activity in rat granulosa cells transfected with an ERE-luciferase...

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Veröffentlicht in:	Endocrinology (Philadelphia) 1999-10, Vol.140 (10), p.4530-4541
Hauptverfasser:	O'Brien, M L, Park, K, In, Y, Park-Sarge, O K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence - genetics Cells, Cultured Estradiol - pharmacology Estrogen Receptor beta Exons - physiology Female Granulosa Cells - drug effects Granulosa Cells - metabolism Luciferases - genetics Luciferases - metabolism Molecular Sequence Data Ovary - chemistry Rats Rats, Sprague-Dawley Receptors, Estrogen - genetics Receptors, Estrogen - metabolism RNA, Messenger - metabolism Tissue Extracts - metabolism Transcription, Genetic Uterus - chemistry
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