Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida

We have isolated a cDNA clone that corresponds to the Ht1 locus of petunia which controls the hydroxylation of dihydrokaempferol to dihydroquercetin and of naringenin to eriodictyol by the action of flavonoid 3'-hydroxylase (F3'H). The cDNA encodes a 512 amino acid polypeptide with regions...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 1999-08, Vol.19 (4), p.441-451
Hauptverfasser:	Brugliera, F, Barri-Rewell, G, Holton, T.A, Mason, J.G
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Amino Acid Sequence amino acid sequences anthers anthocyanins Anthocyanins - metabolism Base Sequence biochemical pathways Biological and medical sciences calyx Classical and quantitative genetics. Population genetics. Molecular genetics Cloning, Molecular complementary DNA corolla Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism enzyme activity Flavanones flavonoids Flavonoids - metabolism flowering flowers Fundamental and applied biological sciences. Psychology genbank/af155332 gene expression Gene Expression Regulation, Plant Generalities. Genetics. Plant material Genes, Plant - genetics Genes. Genome Genetic Complementation Test Genetic Linkage - genetics Genetics and breeding of economic plants Hybridization, Genetic loci messenger RNA Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation - genetics nucleotide sequences ovules oxygenases Petunia hybrida Phylogeny Plant Structures - enzymology Plant Structures - genetics RNA, Messenger - analysis RNA, Messenger - genetics Sequence Homology, Amino Acid Solanaceae - enzymology Solanaceae - genetics stems substrates Yeasts - genetics
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