Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida
We have isolated a cDNA clone that corresponds to the Ht1 locus of petunia which controls the hydroxylation of dihydrokaempferol to dihydroquercetin and of naringenin to eriodictyol by the action of flavonoid 3'-hydroxylase (F3'H). The cDNA encodes a 512 amino acid polypeptide with regions...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 1999-08, Vol.19 (4), p.441-451 |
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| creator | Brugliera, F Barri-Rewell, G Holton, T.A Mason, J.G |
| description | We have isolated a cDNA clone that corresponds to the Ht1 locus of petunia which controls the hydroxylation of dihydrokaempferol to dihydroquercetin and of naringenin to eriodictyol by the action of flavonoid 3'-hydroxylase (F3'H). The cDNA encodes a 512 amino acid polypeptide with regions of similarity to petunia flavonoid 3',5'-hydroxylases (F3'5'H). Both F3'H and F3'5'H are cytochromes P450 and are key enzymes in the flavonoid pathway leading to the production of the coloured anthocyanins. The F3'H transcript is most abundant in petals from flowers at an early stage of development and levels decline as the flower matures. Transcripts are also detected in the ovaries, sepals, peduncles, stems and anthers of the petunia plant. No or very reduced levels of transcripts are detected in ht1/ht1 lines. This is the first report of isolation of a F3'H cDNA clone from any species. |
| doi_str_mv | 10.1046/j.1365-313x.1999.00539.x |
| format | Article |
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The cDNA encodes a 512 amino acid polypeptide with regions of similarity to petunia flavonoid 3',5'-hydroxylases (F3'5'H). Both F3'H and F3'5'H are cytochromes P450 and are key enzymes in the flavonoid pathway leading to the production of the coloured anthocyanins. The F3'H transcript is most abundant in petals from flowers at an early stage of development and levels decline as the flower matures. Transcripts are also detected in the ovaries, sepals, peduncles, stems and anthers of the petunia plant. No or very reduced levels of transcripts are detected in ht1/ht1 lines. This is the first report of isolation of a F3'H cDNA clone from any species.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1046/j.1365-313x.1999.00539.x</identifier><identifier>PMID: 10504566</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; amino acid sequences ; anthers ; anthocyanins ; Anthocyanins - metabolism ; Base Sequence ; biochemical pathways ; Biological and medical sciences ; calyx ; Classical and quantitative genetics. Population genetics. Molecular genetics ; Cloning, Molecular ; complementary DNA ; corolla ; Cytochrome P-450 Enzyme System - chemistry ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; enzyme activity ; Flavanones ; flavonoids ; Flavonoids - metabolism ; flowering ; flowers ; Fundamental and applied biological sciences. Psychology ; genbank/af155332 ; gene expression ; Gene Expression Regulation, Plant ; Generalities. Genetics. Plant material ; Genes, Plant - genetics ; Genes. Genome ; Genetic Complementation Test ; Genetic Linkage - genetics ; Genetics and breeding of economic plants ; Hybridization, Genetic ; loci ; messenger RNA ; Mixed Function Oxygenases - chemistry ; Mixed Function Oxygenases - genetics ; Mixed Function Oxygenases - metabolism ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutation - genetics ; nucleotide sequences ; ovules ; oxygenases ; Petunia hybrida ; Phylogeny ; Plant Structures - enzymology ; Plant Structures - genetics ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Sequence Homology, Amino Acid ; Solanaceae - enzymology ; Solanaceae - genetics ; stems ; substrates ; Yeasts - genetics</subject><ispartof>The Plant journal : for cell and molecular biology, 1999-08, Vol.19 (4), p.441-451</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5699-581a0c06a640bd4fe156a0ab75cc24d26bb0a137e85ca6d5d485332dc24bf2463</citedby><cites>FETCH-LOGICAL-c5699-581a0c06a640bd4fe156a0ab75cc24d26bb0a137e85ca6d5d485332dc24bf2463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-313X.1999.00539.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-313X.1999.00539.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1933067$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10504566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brugliera, F</creatorcontrib><creatorcontrib>Barri-Rewell, G</creatorcontrib><creatorcontrib>Holton, T.A</creatorcontrib><creatorcontrib>Mason, J.G</creatorcontrib><title>Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>We have isolated a cDNA clone that corresponds to the Ht1 locus of petunia which controls the hydroxylation of dihydrokaempferol to dihydroquercetin and of naringenin to eriodictyol by the action of flavonoid 3'-hydroxylase (F3'H). The cDNA encodes a 512 amino acid polypeptide with regions of similarity to petunia flavonoid 3',5'-hydroxylases (F3'5'H). Both F3'H and F3'5'H are cytochromes P450 and are key enzymes in the flavonoid pathway leading to the production of the coloured anthocyanins. The F3'H transcript is most abundant in petals from flowers at an early stage of development and levels decline as the flower matures. Transcripts are also detected in the ovaries, sepals, peduncles, stems and anthers of the petunia plant. No or very reduced levels of transcripts are detected in ht1/ht1 lines. This is the first report of isolation of a F3'H cDNA clone from any species.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>anthers</subject><subject>anthocyanins</subject><subject>Anthocyanins - metabolism</subject><subject>Base Sequence</subject><subject>biochemical pathways</subject><subject>Biological and medical sciences</subject><subject>calyx</subject><subject>Classical and quantitative genetics. Population genetics. Molecular genetics</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>corolla</subject><subject>Cytochrome P-450 Enzyme System - chemistry</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>enzyme activity</subject><subject>Flavanones</subject><subject>flavonoids</subject><subject>Flavonoids - metabolism</subject><subject>flowering</subject><subject>flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/af155332</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genes, Plant - genetics</subject><subject>Genes. Genome</subject><subject>Genetic Complementation Test</subject><subject>Genetic Linkage - genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>Hybridization, Genetic</subject><subject>loci</subject><subject>messenger RNA</subject><subject>Mixed Function Oxygenases - chemistry</subject><subject>Mixed Function Oxygenases - genetics</subject><subject>Mixed Function Oxygenases - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation - genetics</subject><subject>nucleotide sequences</subject><subject>ovules</subject><subject>oxygenases</subject><subject>Petunia hybrida</subject><subject>Phylogeny</subject><subject>Plant Structures - enzymology</subject><subject>Plant Structures - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>Solanaceae - enzymology</subject><subject>Solanaceae - genetics</subject><subject>stems</subject><subject>substrates</subject><subject>Yeasts - genetics</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EosvCXwAfEJwS7PgjicSlKtAWVVCJVuJmTWyn65U3XuwENvz6JmQR3OBijzzPOyP5QQhTklPC5ZttTpkUGaPskNO6rnNCBKvzwwO0-t34-hCtSC1JVnJanKAnKW0JoSWT_DE6oUQQLqRcofEyBQ-9Cx2GzmC9gQi6t9H9XB5DiwG3Hr6HLjiD2etsM5oYDqOHZLF-9-kUax-6qQwx2rQPnXHdHe4D7jcWX_QU-6CHNM-5tv3QOcCbsYnOwFP0qAWf7LPjvUa3H97fnF1kV5_PL89OrzItZF1noqJANJEgOWkMby0VEgg0pdC64KaQTUOAstJWQoM0wvBKMFaYqdm0BZdsjV4tc_cxfBts6tXOJW29h86GIamSlFUhxL_B6fNYWU_HGlULqGNIKdpW7aPbQRwVJWr2o7Zq1qBmDWr2o375UYcp-vy4Y2h21vwVXIRMwMsjAEmDbyN02qU_XM0YkeWEvV2wH87b8b_3q5vrj1MxxV8s8RaCgrs4bbj9UhDKSFHzglaM3QPovrV0</recordid><startdate>199908</startdate><enddate>199908</enddate><creator>Brugliera, F</creator><creator>Barri-Rewell, G</creator><creator>Holton, T.A</creator><creator>Mason, J.G</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199908</creationdate><title>Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida</title><author>Brugliera, F ; Barri-Rewell, G ; Holton, T.A ; Mason, J.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5699-581a0c06a640bd4fe156a0ab75cc24d26bb0a137e85ca6d5d485332dc24bf2463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>anthers</topic><topic>anthocyanins</topic><topic>Anthocyanins - metabolism</topic><topic>Base Sequence</topic><topic>biochemical pathways</topic><topic>Biological and medical sciences</topic><topic>calyx</topic><topic>Classical and quantitative genetics. Population genetics. Molecular genetics</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>corolla</topic><topic>Cytochrome P-450 Enzyme System - chemistry</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>enzyme activity</topic><topic>Flavanones</topic><topic>flavonoids</topic><topic>Flavonoids - metabolism</topic><topic>flowering</topic><topic>flowers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/af155332</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genes, Plant - genetics</topic><topic>Genes. Genome</topic><topic>Genetic Complementation Test</topic><topic>Genetic Linkage - genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>Hybridization, Genetic</topic><topic>loci</topic><topic>messenger RNA</topic><topic>Mixed Function Oxygenases - chemistry</topic><topic>Mixed Function Oxygenases - genetics</topic><topic>Mixed Function Oxygenases - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation - genetics</topic><topic>nucleotide sequences</topic><topic>ovules</topic><topic>oxygenases</topic><topic>Petunia hybrida</topic><topic>Phylogeny</topic><topic>Plant Structures - enzymology</topic><topic>Plant Structures - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>Solanaceae - enzymology</topic><topic>Solanaceae - genetics</topic><topic>stems</topic><topic>substrates</topic><topic>Yeasts - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brugliera, F</creatorcontrib><creatorcontrib>Barri-Rewell, G</creatorcontrib><creatorcontrib>Holton, T.A</creatorcontrib><creatorcontrib>Mason, J.G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brugliera, F</au><au>Barri-Rewell, G</au><au>Holton, T.A</au><au>Mason, J.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>1999-08</date><risdate>1999</risdate><volume>19</volume><issue>4</issue><spage>441</spage><epage>451</epage><pages>441-451</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>We have isolated a cDNA clone that corresponds to the Ht1 locus of petunia which controls the hydroxylation of dihydrokaempferol to dihydroquercetin and of naringenin to eriodictyol by the action of flavonoid 3'-hydroxylase (F3'H). The cDNA encodes a 512 amino acid polypeptide with regions of similarity to petunia flavonoid 3',5'-hydroxylases (F3'5'H). Both F3'H and F3'5'H are cytochromes P450 and are key enzymes in the flavonoid pathway leading to the production of the coloured anthocyanins. The F3'H transcript is most abundant in petals from flowers at an early stage of development and levels decline as the flower matures. Transcripts are also detected in the ovaries, sepals, peduncles, stems and anthers of the petunia plant. No or very reduced levels of transcripts are detected in ht1/ht1 lines. This is the first report of isolation of a F3'H cDNA clone from any species.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>10504566</pmid><doi>10.1046/j.1365-313x.1999.00539.x</doi><tpages>11</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence amino acid sequences anthers anthocyanins Anthocyanins - metabolism Base Sequence biochemical pathways Biological and medical sciences calyx Classical and quantitative genetics. Population genetics. Molecular genetics Cloning, Molecular complementary DNA corolla Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism enzyme activity Flavanones flavonoids Flavonoids - metabolism flowering flowers Fundamental and applied biological sciences. Psychology genbank/af155332 gene expression Gene Expression Regulation, Plant Generalities. Genetics. Plant material Genes, Plant - genetics Genes. Genome Genetic Complementation Test Genetic Linkage - genetics Genetics and breeding of economic plants Hybridization, Genetic loci messenger RNA Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation - genetics nucleotide sequences ovules oxygenases Petunia hybrida Phylogeny Plant Structures - enzymology Plant Structures - genetics RNA, Messenger - analysis RNA, Messenger - genetics Sequence Homology, Amino Acid Solanaceae - enzymology Solanaceae - genetics stems substrates Yeasts - genetics |
| title | Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida |
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