Immune stimulating activity of two new chitosan containing adjuvant formulations
Recombinant proteins have potential as both human and veterinary vaccine antigens, but they are often weakly immunogenic and immunization with recombinant proteins may not elicit a significant immune response that recognizes the native protein. This report describes the immune stimulating activity o...
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| Veröffentlicht in: | Vaccine 2000-11, Vol.19 (6), p.661-668 |
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| creator | Seferian, Peter G Martinez, Mitzi L |
| description | Recombinant proteins have potential as both human and veterinary vaccine antigens, but they are often weakly immunogenic and immunization with recombinant proteins may not elicit a significant immune response that recognizes the native protein. This report describes the immune stimulating activity of two new adjuvant formulations, a zinc-chitosan particle formulation designed to bind to histidine tagged recombinant proteins; and an emulsion formulation containing chitosan. BALB/c mice vaccinated with formulations comprising recombinant beta-human chorionic gonadotropin (βhCG) and each adjuvant had prolonged high titer antibodies that recognized both the recombinant βhCG and native hCG. βhCG is an established target for immunocontraceptive vaccines and a potential target for tumor immunotherapy. Isotype analysis of these antibodies revealed an IgG1 response in mice immunized with zinc-chitosan particles and a mixed IgG1, IgG2a and IgG2b response with the emulsion. These chitosan based adjuvant formulations were effective in sensitizing mice and guinea pigs for antigen specific DTH responses, indicating that these adjuvants stimulate both B and T lymphocytes. The ability of these adjuvants to stimulate significant responses with a poorly immunogenic recombinant protein suggests that they may have potential in developing vaccines based on synthetic peptides and subunit antigens. |
| doi_str_mv | 10.1016/S0264-410X(00)00248-6 |
| format | Article |
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This report describes the immune stimulating activity of two new adjuvant formulations, a zinc-chitosan particle formulation designed to bind to histidine tagged recombinant proteins; and an emulsion formulation containing chitosan. BALB/c mice vaccinated with formulations comprising recombinant beta-human chorionic gonadotropin (βhCG) and each adjuvant had prolonged high titer antibodies that recognized both the recombinant βhCG and native hCG. βhCG is an established target for immunocontraceptive vaccines and a potential target for tumor immunotherapy. Isotype analysis of these antibodies revealed an IgG1 response in mice immunized with zinc-chitosan particles and a mixed IgG1, IgG2a and IgG2b response with the emulsion. These chitosan based adjuvant formulations were effective in sensitizing mice and guinea pigs for antigen specific DTH responses, indicating that these adjuvants stimulate both B and T lymphocytes. The ability of these adjuvants to stimulate significant responses with a poorly immunogenic recombinant protein suggests that they may have potential in developing vaccines based on synthetic peptides and subunit antigens.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(00)00248-6</identifier><identifier>PMID: 11090719</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvant ; Adjuvants, Immunologic - pharmacology ; Animals ; Applied microbiology ; b-chorionic gonadotropin ; Biological and medical sciences ; Chelating Agents - pharmacology ; Chitin - analogs & derivatives ; Chitin - immunology ; Chitin - pharmacology ; Chitosan ; Chorionic Gonadotropin, beta Subunit, Human - immunology ; Chorionic Gonadotropin, beta Subunit, Human - pharmacology ; Emulsions - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Histidine ; Hypersensitivity, Delayed ; Immunoglobulin G - blood ; Immunoglobulins - blood ; Mice ; Mice, Inbred BALB C ; Microbiology ; Rabbits ; Recombinant Proteins - immunology ; Recombinant Proteins - pharmacology ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology ; Vaccination ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Zinc - pharmacology</subject><ispartof>Vaccine, 2000-11, Vol.19 (6), p.661-668</ispartof><rights>2001 Elsevier Science Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-6103dfd69bb56e8ee572b274028fd80512d5c07164642b6a2bde2f5c728f92913</citedby><cites>FETCH-LOGICAL-c538t-6103dfd69bb56e8ee572b274028fd80512d5c07164642b6a2bde2f5c728f92913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0264-410X(00)00248-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995,64387</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=851451$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11090719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nara, P</contributor><contributor>Rabinovich, R</contributor><contributor>Weniger, B (eds)</contributor><contributor>Martone, W</contributor><creatorcontrib>Seferian, Peter G</creatorcontrib><creatorcontrib>Martinez, Mitzi L</creatorcontrib><title>Immune stimulating activity of two new chitosan containing adjuvant formulations</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Recombinant proteins have potential as both human and veterinary vaccine antigens, but they are often weakly immunogenic and immunization with recombinant proteins may not elicit a significant immune response that recognizes the native protein. This report describes the immune stimulating activity of two new adjuvant formulations, a zinc-chitosan particle formulation designed to bind to histidine tagged recombinant proteins; and an emulsion formulation containing chitosan. BALB/c mice vaccinated with formulations comprising recombinant beta-human chorionic gonadotropin (βhCG) and each adjuvant had prolonged high titer antibodies that recognized both the recombinant βhCG and native hCG. βhCG is an established target for immunocontraceptive vaccines and a potential target for tumor immunotherapy. Isotype analysis of these antibodies revealed an IgG1 response in mice immunized with zinc-chitosan particles and a mixed IgG1, IgG2a and IgG2b response with the emulsion. These chitosan based adjuvant formulations were effective in sensitizing mice and guinea pigs for antigen specific DTH responses, indicating that these adjuvants stimulate both B and T lymphocytes. The ability of these adjuvants to stimulate significant responses with a poorly immunogenic recombinant protein suggests that they may have potential in developing vaccines based on synthetic peptides and subunit antigens.</description><subject>Adjuvant</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Animals</subject><subject>Applied microbiology</subject><subject>b-chorionic gonadotropin</subject><subject>Biological and medical sciences</subject><subject>Chelating Agents - pharmacology</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - immunology</subject><subject>Chitin - pharmacology</subject><subject>Chitosan</subject><subject>Chorionic Gonadotropin, beta Subunit, Human - immunology</subject><subject>Chorionic Gonadotropin, beta Subunit, Human - pharmacology</subject><subject>Emulsions - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Histidine</subject><subject>Hypersensitivity, Delayed</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulins - blood</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Rabbits</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - pharmacology</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><subject>Vaccination</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Zinc - pharmacology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3TAQRkVoSW5v8hNSDIXSLtyMZOvhVSmhj0CghaTQnZClcatgS6kk35B_X98H6TKrWcz5NJ8OIecUPlCg4uIGmGjrlsKvdwDvAViranFEVlTJpmacqhdk9YSckFc53wEAb2h3TE4ohQ4k7Vbkx9U0zQGrXPw0j6b48LsytviNL49VHKryEKuAD5X940vMJlQ2hmJ82HHubt6YUKohpn04hnxKXg5mzHh2mGvy88vn28tv9fX3r1eXn65ryxtVakGhcYMTXd9zgQqRS9Yz2QJTg1PAKXPcLhVFK1rWC8N6h2zgVi77jnW0WZO3-3fvU_w7Yy568tniOJqAcc5agpScd-pZkEq13F7qrAnfgzbFnBMO-j75yaRHTUFvneudc70VqgH0zrkWS-714cDcT-j-pw6SF-DNATDZmnFIJlifnzjFacu3H_q4p3CxtvGYdLYeg0XnE9qiXfTPFPkHoUCd9g</recordid><startdate>20001108</startdate><enddate>20001108</enddate><creator>Seferian, Peter G</creator><creator>Martinez, Mitzi L</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20001108</creationdate><title>Immune stimulating activity of two new chitosan containing adjuvant formulations</title><author>Seferian, Peter G ; Martinez, Mitzi L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-6103dfd69bb56e8ee572b274028fd80512d5c07164642b6a2bde2f5c728f92913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adjuvant</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>b-chorionic gonadotropin</topic><topic>Biological and medical sciences</topic><topic>Chelating Agents - pharmacology</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - immunology</topic><topic>Chitin - pharmacology</topic><topic>Chitosan</topic><topic>Chorionic Gonadotropin, beta Subunit, Human - immunology</topic><topic>Chorionic Gonadotropin, beta Subunit, Human - pharmacology</topic><topic>Emulsions - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Histidine</topic><topic>Hypersensitivity, Delayed</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulins - blood</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Rabbits</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - pharmacology</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - immunology</topic><topic>Vaccination</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Zinc - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seferian, Peter G</creatorcontrib><creatorcontrib>Martinez, Mitzi L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seferian, Peter G</au><au>Martinez, Mitzi L</au><au>Nara, P</au><au>Rabinovich, R</au><au>Weniger, B (eds)</au><au>Martone, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune stimulating activity of two new chitosan containing adjuvant formulations</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2000-11-08</date><risdate>2000</risdate><volume>19</volume><issue>6</issue><spage>661</spage><epage>668</epage><pages>661-668</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Recombinant proteins have potential as both human and veterinary vaccine antigens, but they are often weakly immunogenic and immunization with recombinant proteins may not elicit a significant immune response that recognizes the native protein. This report describes the immune stimulating activity of two new adjuvant formulations, a zinc-chitosan particle formulation designed to bind to histidine tagged recombinant proteins; and an emulsion formulation containing chitosan. BALB/c mice vaccinated with formulations comprising recombinant beta-human chorionic gonadotropin (βhCG) and each adjuvant had prolonged high titer antibodies that recognized both the recombinant βhCG and native hCG. βhCG is an established target for immunocontraceptive vaccines and a potential target for tumor immunotherapy. Isotype analysis of these antibodies revealed an IgG1 response in mice immunized with zinc-chitosan particles and a mixed IgG1, IgG2a and IgG2b response with the emulsion. These chitosan based adjuvant formulations were effective in sensitizing mice and guinea pigs for antigen specific DTH responses, indicating that these adjuvants stimulate both B and T lymphocytes. The ability of these adjuvants to stimulate significant responses with a poorly immunogenic recombinant protein suggests that they may have potential in developing vaccines based on synthetic peptides and subunit antigens.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11090719</pmid><doi>10.1016/S0264-410X(00)00248-6</doi><tpages>8</tpages></addata></record> |
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| subjects | Adjuvant Adjuvants, Immunologic - pharmacology Animals Applied microbiology b-chorionic gonadotropin Biological and medical sciences Chelating Agents - pharmacology Chitin - analogs & derivatives Chitin - immunology Chitin - pharmacology Chitosan Chorionic Gonadotropin, beta Subunit, Human - immunology Chorionic Gonadotropin, beta Subunit, Human - pharmacology Emulsions - pharmacology Female Fundamental and applied biological sciences. Psychology Guinea Pigs Histidine Hypersensitivity, Delayed Immunoglobulin G - blood Immunoglobulins - blood Mice Mice, Inbred BALB C Microbiology Rabbits Recombinant Proteins - immunology Recombinant Proteins - pharmacology T-Lymphocytes - drug effects T-Lymphocytes - immunology Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Zinc - pharmacology |
| title | Immune stimulating activity of two new chitosan containing adjuvant formulations |
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