Determination of icariin in rat plasma by reverse-phase high-performance liquid chromatography after oral administration of a lipid-based suspension of Epimedium koreanum extract
A simple, specific and sensitive method for quantitative determination of icariin in rat plasma using reverse‐phase high‐performance liquid chromatography with UV‐detection was developed and applied to an animal study of a lipid‐based suspension of the Epimedium koreanum extract in rats. Rutin was s...
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| Veröffentlicht in: | Biomedical chromatography 2008-06, Vol.22 (6), p.625-629 |
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| container_title | Biomedical chromatography |
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| creator | Pozharitskaya, Olga N. Karlina, Marina V. Shikov, Alexander N. Kosman, Vera M. Makarova, Marina N. Makarov, Valery G. |
| description | A simple, specific and sensitive method for quantitative determination of icariin in rat plasma using reverse‐phase high‐performance liquid chromatography with UV‐detection was developed and applied to an animal study of a lipid‐based suspension of the Epimedium koreanum extract in rats. Rutin was selected as the internal standard and methanol was found to be the best solvent for extraction of icariin from the plasma. Linearity was observed between 0.030 and 100 µg/mL (r > 0.99). The extraction recoveries of icariin and rutin were approximately 75 and 80%, respectively, in plasma. The intra‐ and inter‐day coefficients of variation were less than 5%. The limit of detection was 6 ng/mL and the limit of quantification was 18 ng/mL. Copyright © 2008 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/bmc.978 |
| format | Article |
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Rutin was selected as the internal standard and methanol was found to be the best solvent for extraction of icariin from the plasma. Linearity was observed between 0.030 and 100 µg/mL (r > 0.99). The extraction recoveries of icariin and rutin were approximately 75 and 80%, respectively, in plasma. The intra‐ and inter‐day coefficients of variation were less than 5%. The limit of detection was 6 ng/mL and the limit of quantification was 18 ng/mL. Copyright © 2008 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.978</identifier><identifier>PMID: 18254138</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Administration, Oral ; Animals ; Chromatography, High Pressure Liquid - methods ; Epimedium - chemistry ; Epimedium koreanum extract ; Flavonoids - blood ; icariin ; Lipids - chemistry ; Male ; Plant Extracts - administration & dosage ; Plant Extracts - chemistry ; rat plasma ; Rats ; Rats, Wistar ; Reproducibility of Results ; RP-HPLC ; Sensitivity and Specificity</subject><ispartof>Biomedical chromatography, 2008-06, Vol.22 (6), p.625-629</ispartof><rights>Copyright © 2008 John Wiley & Sons, Ltd.</rights><rights>Copyright (c) 2008 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-6add583f45b17ed796018f1fafc35890db711643a4e9c6ca60a3e47469972e053</citedby><cites>FETCH-LOGICAL-c3538-6add583f45b17ed796018f1fafc35890db711643a4e9c6ca60a3e47469972e053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.978$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.978$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18254138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pozharitskaya, Olga N.</creatorcontrib><creatorcontrib>Karlina, Marina V.</creatorcontrib><creatorcontrib>Shikov, Alexander N.</creatorcontrib><creatorcontrib>Kosman, Vera M.</creatorcontrib><creatorcontrib>Makarova, Marina N.</creatorcontrib><creatorcontrib>Makarov, Valery G.</creatorcontrib><title>Determination of icariin in rat plasma by reverse-phase high-performance liquid chromatography after oral administration of a lipid-based suspension of Epimedium koreanum extract</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>A simple, specific and sensitive method for quantitative determination of icariin in rat plasma using reverse‐phase high‐performance liquid chromatography with UV‐detection was developed and applied to an animal study of a lipid‐based suspension of the Epimedium koreanum extract in rats. Rutin was selected as the internal standard and methanol was found to be the best solvent for extraction of icariin from the plasma. Linearity was observed between 0.030 and 100 µg/mL (r > 0.99). The extraction recoveries of icariin and rutin were approximately 75 and 80%, respectively, in plasma. The intra‐ and inter‐day coefficients of variation were less than 5%. The limit of detection was 6 ng/mL and the limit of quantification was 18 ng/mL. Copyright © 2008 John Wiley & Sons, Ltd.</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Epimedium - chemistry</subject><subject>Epimedium koreanum extract</subject><subject>Flavonoids - blood</subject><subject>icariin</subject><subject>Lipids - chemistry</subject><subject>Male</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - chemistry</subject><subject>rat plasma</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reproducibility of Results</subject><subject>RP-HPLC</subject><subject>Sensitivity and Specificity</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EoktBvAHyCQ4orR0ncXyEbWmR2nIAhMTFmjiTxjSOUzsp3dfiCXGVVTkhWbKl-eYbjX9CXnN2xBnLjxtnjpSsn5ANZ0plrGb8KdmwvFKZqKU6IC9i_MUYU1Uun5MDXudlwUW9IX9OcMbg7Aiz9SP1HbUGgrUjTSfATKcBogPa7GjAOwwRs6mHiLS31302Yeh8cDAapIO9XWxLTR-8g9lfB5j6HYUu6akPMFBo0xgb5_A4ClLTZNusScKWxiVOOMZ97XSyDlu7OHrjA8KYHnifes38kjzrYIj4an8fku-fTr9tz7OLL2eftx8uMiNKUWcVtG1Zi64oGy6xlapivO54B12q14q1jeS8KgQUqExloGIgsJBFpZTMkZXikLxdvVPwtwvGWTsbDQ4DjOiXqCWTUrDiAXy3gib4GAN2egrWQdhpzvRDPDrFo1M8iXyzVy5N2u4ft88jAe9X4LcdcPc_j_54uV112UqnX8X7RxrCja6kkKX-cXWmy59c8vOrr1qIv9M0rD0</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Pozharitskaya, Olga N.</creator><creator>Karlina, Marina V.</creator><creator>Shikov, Alexander N.</creator><creator>Kosman, Vera M.</creator><creator>Makarova, Marina N.</creator><creator>Makarov, Valery G.</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>Determination of icariin in rat plasma by reverse-phase high-performance liquid chromatography after oral administration of a lipid-based suspension of Epimedium koreanum extract</title><author>Pozharitskaya, Olga N. ; Karlina, Marina V. ; Shikov, Alexander N. ; Kosman, Vera M. ; Makarova, Marina N. ; Makarov, Valery G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-6add583f45b17ed796018f1fafc35890db711643a4e9c6ca60a3e47469972e053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Administration, Oral</topic><topic>Animals</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Epimedium - chemistry</topic><topic>Epimedium koreanum extract</topic><topic>Flavonoids - blood</topic><topic>icariin</topic><topic>Lipids - chemistry</topic><topic>Male</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - chemistry</topic><topic>rat plasma</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reproducibility of Results</topic><topic>RP-HPLC</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pozharitskaya, Olga N.</creatorcontrib><creatorcontrib>Karlina, Marina V.</creatorcontrib><creatorcontrib>Shikov, Alexander N.</creatorcontrib><creatorcontrib>Kosman, Vera M.</creatorcontrib><creatorcontrib>Makarova, Marina N.</creatorcontrib><creatorcontrib>Makarov, Valery G.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pozharitskaya, Olga N.</au><au>Karlina, Marina V.</au><au>Shikov, Alexander N.</au><au>Kosman, Vera M.</au><au>Makarova, Marina N.</au><au>Makarov, Valery G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of icariin in rat plasma by reverse-phase high-performance liquid chromatography after oral administration of a lipid-based suspension of Epimedium koreanum extract</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2008-06</date><risdate>2008</risdate><volume>22</volume><issue>6</issue><spage>625</spage><epage>629</epage><pages>625-629</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>A simple, specific and sensitive method for quantitative determination of icariin in rat plasma using reverse‐phase high‐performance liquid chromatography with UV‐detection was developed and applied to an animal study of a lipid‐based suspension of the Epimedium koreanum extract in rats. Rutin was selected as the internal standard and methanol was found to be the best solvent for extraction of icariin from the plasma. Linearity was observed between 0.030 and 100 µg/mL (r > 0.99). The extraction recoveries of icariin and rutin were approximately 75 and 80%, respectively, in plasma. The intra‐ and inter‐day coefficients of variation were less than 5%. The limit of detection was 6 ng/mL and the limit of quantification was 18 ng/mL. Copyright © 2008 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>18254138</pmid><doi>10.1002/bmc.978</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biomedical chromatography, 2008-06, Vol.22 (6), p.625-629 |
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| source | MEDLINE; Wiley Journals |
| subjects | Administration, Oral Animals Chromatography, High Pressure Liquid - methods Epimedium - chemistry Epimedium koreanum extract Flavonoids - blood icariin Lipids - chemistry Male Plant Extracts - administration & dosage Plant Extracts - chemistry rat plasma Rats Rats, Wistar Reproducibility of Results RP-HPLC Sensitivity and Specificity |
| title | Determination of icariin in rat plasma by reverse-phase high-performance liquid chromatography after oral administration of a lipid-based suspension of Epimedium koreanum extract |
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