Kinetic Isotope Effect Studies of the Reaction Catalyzed by Uracil DNA Glycosylase: Evidence for an Oxocarbenium Ion−Uracil Anion Intermediate

The DNA repair enzyme uracil DNA glycosylase catalyzes the first step in the uracil base excision repair pathway, the hydrolytic cleavage of the N-glycosidic bond of deoxyuridine in DNA. Here we report kinetic isotope effect (KIE) measurements that have allowed the determination of the transition-st...

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Veröffentlicht in:	Biochemistry (Easton) 2000-11, Vol.39 (46), p.14054-14064
Hauptverfasser:	Werner, R. Marshall, Stivers, James T
Format:	Artikel
Sprache:	eng
Schlagworte:	Anions base excision repair Binding Sites Catalysis Chromatography, High Pressure Liquid deoxyuridine Deoxyuridine - chemistry Deuterium DNA Glycosylases DNA Repair DNA, Single-Stranded - chemical synthesis Glycosides - chemistry Kinetics N-Glycosyl Hydrolases - chemistry Static Electricity Substrate Specificity Tritium - chemistry Uracil - chemistry Uracil-DNA Glycosidase uracil-DNA glycosylase
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