Reconstitution of the Vertebrate Visual Cascade Using Recombinant Heterotrimeric Transducin Purified from Sf9 Cells

For reconstitution studies with rhodopsin and cGMP phosphodiesterase (PDE), all three subunits of heterotrimeric transducin (Tαβγ) were simultaneously expressed in Sf9 cells at high levels using a baculovirus expression system and purified to homogeneity. Light-activated rhodopsin catalyzed the load...

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Veröffentlicht in:	Protein expression and purification 2000-12, Vol.20 (3), p.514-526
Hauptverfasser:	Min, K.Christopher, Gravina, Stephen A., Sakmar, Thomas P.
Format:	Artikel
Sprache:	eng
Schlagworte:	3',5'-Cyclic-GMP Phosphodiesterases - metabolism Animals Cattle Cell Line Cloning, Molecular Enzyme Activation Genetic Vectors Guanosine 5'-O-(3-Thiotriphosphate) - metabolism Heterotrimeric GTP-Binding Proteins - metabolism Protein Binding Recombinant Proteins - genetics Recombinant Proteins - metabolism Rhodopsin - metabolism Solutions Spodoptera Transducin - genetics Transducin - metabolism Vision, Ocular - physiology
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