Heterologous expression of the alcohol dehydrogenase ( adhI) gene from Geobacillus thermoglucosidasius strain M10EXG

A thermostable alcohol dehydrogenase (ADH-I) isolated from the potential thermophilic ethanologen Geobacillus thermoglucosidasius strain M10EXG has been characterised. Inverse PCR showed that the gene ( adhI) was localised with 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3 hexuloisomerase (P...

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Veröffentlicht in:	Journal of biotechnology 2008-06, Vol.135 (2), p.127-133
Hauptverfasser:	Jeon, Young Jae, Fong, Jiunn C.N., Riyanti, Eny I., Neilan, Brett A., Rogers, Peter L., Svenson, Charles J.
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Butanol - metabolism 1-Octanol - metabolism 2-Propanol - metabolism adhI gene alcohol dehydrogenase Alcohol Dehydrogenase - classification Alcohol Dehydrogenase - genetics Alcohol Dehydrogenase - metabolism Aldehyde-Lyases - genetics Aldehyde-Lyases - metabolism Aldose-Ketose Isomerases - genetics Amino Acid Sequence Bacillaceae - enzymology Bacillaceae - genetics Bacillus thermoglucosidasius Bacterial Proteins - classification Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological and medical sciences Biotechnology DNA, Bacterial - chemistry DNA, Bacterial - genetics Electrophoresis, Polyacrylamide Gel enzyme activity Escherichia coli Escherichia coli - genetics Ethanol - metabolism Ethanol production Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic gene overexpression gene transfer genes genetic transformation Geobacillus Geobacillus thermoglucosidasius Heptanol - metabolism Heterologous expression Hexanols - metabolism Models, Molecular Molecular Sequence Data Pentanols - metabolism Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA Sequence Homology, Amino Acid Thermophile Thermophilic alcohol dehydrogenase thermophilic bacteria
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