MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation
MicroRNAs (miRNAs) are small RNAs that function as posttranscriptional regulators of gene expression. miRNAs affect a variety of signaling pathways, and impaired miRNA regulation may contribute to the development of cancer and other diseases. Here we show that miRNA miR-10a interacts with the 5'...
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| Veröffentlicht in: | Molecular cell 2008-05, Vol.30 (4), p.460-471 |
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| container_title | Molecular cell |
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| creator | Ørom, Ulf Andersson Nielsen, Finn Cilius Lund, Anders H |
| description | MicroRNAs (miRNAs) are small RNAs that function as posttranscriptional regulators of gene expression. miRNAs affect a variety of signaling pathways, and impaired miRNA regulation may contribute to the development of cancer and other diseases. Here we show that miRNA miR-10a interacts with the 5' untranslated region of mRNAs encoding ribosomal proteins to enhance their translation. miR-10a alleviates translational repression of the ribosomal protein mRNAs during amino acid starvation and is required for their translational induction following anisomycin treatment or overexpression of RAS. We show that miR-10a binds immediately downstream of the regulatory 5'TOP motif and that the 5'TOP regulatory complex and miR-10a are functionally interconnected. The results show that miR-10a may positively control global protein synthesis via the stimulation of ribosomal protein mRNA translation and ribosome biogenesis and hereby affect the ability of cells to undergo transformation. |
| doi_str_mv | 10.1016/j.molcel.2008.05.001 |
| format | Article |
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| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | 5' Untranslated Regions Amino Acids - metabolism Animals Base Sequence Binding Sites Cell Transformation, Neoplastic HeLa Cells Humans Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Mice Molecular Sequence Data NIH 3T3 Cells Protein Biosynthesis Rats Receptors, Immunologic - genetics Receptors, Immunologic - metabolism Ribosomal Proteins - genetics Ribosomal Proteins - metabolism |
| title | MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation |
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