Distribution of 15 Human Kallikreins in Tissues and Biological Fluids
Kallikreins (KLKs) are a group of 15 secreted serine proteases. Some KLKs are established or candidate cancer biomarkers, but for most the physiological function is unknown. We characterized the protein and mRNA abundance patterns of all 15 KLKs in multiple panels of human tissues and biological flu...
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 2007-08, Vol.53 (8), p.1423-1432 |
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| description | Kallikreins (KLKs) are a group of 15 secreted serine proteases. Some KLKs are established or candidate cancer biomarkers, but for most the physiological function is unknown. We characterized the protein and mRNA abundance patterns of all 15 KLKs in multiple panels of human tissues and biological fluids.
We used sensitive and specific sandwich-type ELISAs for each KLK. Reverse transcription PCR was used for transcript amplification. Multiple panels of human tissue extracts (adult and fetal) were tested, along with various biological fluids.
Quantitative protein expression data on 7 sets of adult and 3 sets of fetal tissues were collected for all 15 KLKs. KLKs were also quantified in the following biological fluids: seminal plasma, breast milk, follicular fluid, breast cyst fluid, breast cancer cytosol, amniotic fluid, ovarian cancer ascites, cerebrospinal fluid, cervicovaginal fluid, and urine. The data were used to generate heat maps of KLK concentrations in tissues and fluids and categorize KLK abundance as highly restricted (KLK2 and KLK3 in prostate), restricted (KLK5 in skin, salivary gland, breast, and esophagus; KLK6 in brain and central nervous system; KLK7 in esophagus, heart, liver, and skin; KLK8 in breast, esophagus, skin, and tonsil; KLK13 in esophagus and tonsil), or wide (KLKs 1, 4, 9, 10, 11, 12, 14, and 15).
Quantitative KLK concentrations in tissues and fluids aid in the elucidation of KLK function, and coexpression patterns provide clues for KLK participation in proteolytic cascades. |
| doi_str_mv | 10.1373/clinchem.2007.088104 |
| format | Article |
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We used sensitive and specific sandwich-type ELISAs for each KLK. Reverse transcription PCR was used for transcript amplification. Multiple panels of human tissue extracts (adult and fetal) were tested, along with various biological fluids.
Quantitative protein expression data on 7 sets of adult and 3 sets of fetal tissues were collected for all 15 KLKs. KLKs were also quantified in the following biological fluids: seminal plasma, breast milk, follicular fluid, breast cyst fluid, breast cancer cytosol, amniotic fluid, ovarian cancer ascites, cerebrospinal fluid, cervicovaginal fluid, and urine. The data were used to generate heat maps of KLK concentrations in tissues and fluids and categorize KLK abundance as highly restricted (KLK2 and KLK3 in prostate), restricted (KLK5 in skin, salivary gland, breast, and esophagus; KLK6 in brain and central nervous system; KLK7 in esophagus, heart, liver, and skin; KLK8 in breast, esophagus, skin, and tonsil; KLK13 in esophagus and tonsil), or wide (KLKs 1, 4, 9, 10, 11, 12, 14, and 15).
Quantitative KLK concentrations in tissues and fluids aid in the elucidation of KLK function, and coexpression patterns provide clues for KLK participation in proteolytic cascades.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1373/clinchem.2007.088104</identifier><identifier>PMID: 17573418</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Adult ; Aged ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Body fluids ; Body Fluids - metabolism ; Breast milk ; Central nervous system ; Enzyme-Linked Immunosorbent Assay ; Esophagus ; Female ; Fetus - metabolism ; Fetuses ; Fundamental and applied biological sciences. Psychology ; Heart attacks ; Heart failure ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Kallikreins - genetics ; Kallikreins - metabolism ; Medical sciences ; Middle Aged ; Monoclonal antibodies ; Nervous system ; Organ Specificity ; Ovarian cancer ; Plasma ; Proteases ; RNA, Messenger - metabolism ; Sensitivity and Specificity ; Thyroid gland ; Tissue Extracts - metabolism ; Tissues</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2007-08, Vol.53 (8), p.1423-1432</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright American Association for Clinical Chemistry Aug 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-f9bc20dd85f9920f86c99a6b824ee9f9df4804b2b6d9b31109d19904c1b78d433</citedby><cites>FETCH-LOGICAL-c536t-f9bc20dd85f9920f86c99a6b824ee9f9df4804b2b6d9b31109d19904c1b78d433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18957351$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17573418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shaw, Julie L.V</creatorcontrib><creatorcontrib>Diamandis, Eleftherios P</creatorcontrib><title>Distribution of 15 Human Kallikreins in Tissues and Biological Fluids</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>Kallikreins (KLKs) are a group of 15 secreted serine proteases. Some KLKs are established or candidate cancer biomarkers, but for most the physiological function is unknown. We characterized the protein and mRNA abundance patterns of all 15 KLKs in multiple panels of human tissues and biological fluids.
We used sensitive and specific sandwich-type ELISAs for each KLK. Reverse transcription PCR was used for transcript amplification. Multiple panels of human tissue extracts (adult and fetal) were tested, along with various biological fluids.
Quantitative protein expression data on 7 sets of adult and 3 sets of fetal tissues were collected for all 15 KLKs. KLKs were also quantified in the following biological fluids: seminal plasma, breast milk, follicular fluid, breast cyst fluid, breast cancer cytosol, amniotic fluid, ovarian cancer ascites, cerebrospinal fluid, cervicovaginal fluid, and urine. The data were used to generate heat maps of KLK concentrations in tissues and fluids and categorize KLK abundance as highly restricted (KLK2 and KLK3 in prostate), restricted (KLK5 in skin, salivary gland, breast, and esophagus; KLK6 in brain and central nervous system; KLK7 in esophagus, heart, liver, and skin; KLK8 in breast, esophagus, skin, and tonsil; KLK13 in esophagus and tonsil), or wide (KLKs 1, 4, 9, 10, 11, 12, 14, and 15).
Quantitative KLK concentrations in tissues and fluids aid in the elucidation of KLK function, and coexpression patterns provide clues for KLK participation in proteolytic cascades.</description><subject>Adult</subject><subject>Aged</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Body fluids</subject><subject>Body Fluids - metabolism</subject><subject>Breast milk</subject><subject>Central nervous system</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Esophagus</subject><subject>Female</subject><subject>Fetus - metabolism</subject><subject>Fetuses</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart attacks</subject><subject>Heart failure</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kallikreins - genetics</subject><subject>Kallikreins - metabolism</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Monoclonal antibodies</subject><subject>Nervous system</subject><subject>Organ Specificity</subject><subject>Ovarian cancer</subject><subject>Plasma</subject><subject>Proteases</subject><subject>RNA, Messenger - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Thyroid gland</subject><subject>Tissue Extracts - metabolism</subject><subject>Tissues</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkVFrFDEQx4Mo9qx-A5FF0Le9ZjbJJvOotbVioS_1OWSTbC81m63JLUe_vSl3cuBLH8IQ-M2fmfkR8h7oGphkZzaGZDd-WneUyjVVCih_QVYgGG2V6OElWVFKsUXg8oS8KeW-frlU_WtyAlJIxkGtyMW3ULY5DMs2zKmZxwZEc7VMJjU_TYzhd_YhlSak5jaUsvjSmOSar2GO812wJjaXcQmuvCWvRhOLf3eop-TX5cXt-VV7ffP9x_mX69YK1m_bEQfbUeeUGBE7OqreIpp-UB33Hkd0I1eUD93QOxwYAEUHiJRbGKRynLFT8nmf-5DnP3WarZ5CsT5Gk_y8FC2p7BnW9xwIKLmUHCr48T_wfl5yqkvoDhgq7ARWiO8hm-dSsh_1Qw6TyY8aqH6Sof_J0E8y9F5GbftwyF6Gybtj0-H6Ffh0AEypxxyzSTaUI6ewggKO22zC3WYXstdlqnZqLOjdbieYVhp4x9hfZvufng</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Shaw, Julie L.V</creator><creator>Diamandis, Eleftherios P</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4U-</scope><scope>7QO</scope><scope>7RV</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20070801</creationdate><title>Distribution of 15 Human Kallikreins in Tissues and Biological Fluids</title><author>Shaw, Julie L.V ; Diamandis, Eleftherios P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-f9bc20dd85f9920f86c99a6b824ee9f9df4804b2b6d9b31109d19904c1b78d433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Body fluids</topic><topic>Body Fluids - metabolism</topic><topic>Breast milk</topic><topic>Central nervous system</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Esophagus</topic><topic>Female</topic><topic>Fetus - metabolism</topic><topic>Fetuses</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart attacks</topic><topic>Heart failure</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kallikreins - genetics</topic><topic>Kallikreins - metabolism</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Monoclonal antibodies</topic><topic>Nervous system</topic><topic>Organ Specificity</topic><topic>Ovarian cancer</topic><topic>Plasma</topic><topic>Proteases</topic><topic>RNA, Messenger - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Thyroid gland</topic><topic>Tissue Extracts - metabolism</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shaw, Julie L.V</creatorcontrib><creatorcontrib>Diamandis, Eleftherios P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>University Readers</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shaw, Julie L.V</au><au>Diamandis, Eleftherios P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of 15 Human Kallikreins in Tissues and Biological Fluids</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>53</volume><issue>8</issue><spage>1423</spage><epage>1432</epage><pages>1423-1432</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>Kallikreins (KLKs) are a group of 15 secreted serine proteases. Some KLKs are established or candidate cancer biomarkers, but for most the physiological function is unknown. We characterized the protein and mRNA abundance patterns of all 15 KLKs in multiple panels of human tissues and biological fluids.
We used sensitive and specific sandwich-type ELISAs for each KLK. Reverse transcription PCR was used for transcript amplification. Multiple panels of human tissue extracts (adult and fetal) were tested, along with various biological fluids.
Quantitative protein expression data on 7 sets of adult and 3 sets of fetal tissues were collected for all 15 KLKs. KLKs were also quantified in the following biological fluids: seminal plasma, breast milk, follicular fluid, breast cyst fluid, breast cancer cytosol, amniotic fluid, ovarian cancer ascites, cerebrospinal fluid, cervicovaginal fluid, and urine. The data were used to generate heat maps of KLK concentrations in tissues and fluids and categorize KLK abundance as highly restricted (KLK2 and KLK3 in prostate), restricted (KLK5 in skin, salivary gland, breast, and esophagus; KLK6 in brain and central nervous system; KLK7 in esophagus, heart, liver, and skin; KLK8 in breast, esophagus, skin, and tonsil; KLK13 in esophagus and tonsil), or wide (KLKs 1, 4, 9, 10, 11, 12, 14, and 15).
Quantitative KLK concentrations in tissues and fluids aid in the elucidation of KLK function, and coexpression patterns provide clues for KLK participation in proteolytic cascades.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>17573418</pmid><doi>10.1373/clinchem.2007.088104</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical chemistry (Baltimore, Md.), 2007-08, Vol.53 (8), p.1423-1432 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Adult Aged Analytical, structural and metabolic biochemistry Biological and medical sciences Body fluids Body Fluids - metabolism Breast milk Central nervous system Enzyme-Linked Immunosorbent Assay Esophagus Female Fetus - metabolism Fetuses Fundamental and applied biological sciences. Psychology Heart attacks Heart failure Humans Investigative techniques, diagnostic techniques (general aspects) Kallikreins - genetics Kallikreins - metabolism Medical sciences Middle Aged Monoclonal antibodies Nervous system Organ Specificity Ovarian cancer Plasma Proteases RNA, Messenger - metabolism Sensitivity and Specificity Thyroid gland Tissue Extracts - metabolism Tissues |
| title | Distribution of 15 Human Kallikreins in Tissues and Biological Fluids |
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