Species identification of animal cells by nested PCR targeted to mitochondrial DNA

We developed a highly sensitive and convenient method of nested polymerase chain reaction (PCR) targeted to mitochondrial deoxyribonucleic acid (DNA) to identify animal species quickly in cultured cells. Fourteen vertebrate species, including human, cynomolgus monkey, African green monkey, mouse, ra...

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Veröffentlicht in:	In vitro cellular & developmental biology. Animal 2007-05, Vol.43 (5), p.168-175
Hauptverfasser:	Ono, Kazumi, Satoh, Motonobu, Yoshida, Touho, Ozawa, Yutaka, Kohara, Arihiro, Takeuchi, Masao, Mizusawa, Hiroshi, Sawada, Hidekazu
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Animals Base Sequence Bio-resources Biotechnology Cell Line Cell line authentication Cell lines Chickens Cross-contamination Cynomolgus DNA DNA Primers - metabolism DNA, Mitochondrial - genetics Electrophoresis, Agar Gel Genome Humans Hybridization, Genetic Mitochondrial DNA Molecular Sequence Data Monkeys Polymerase chain reaction Polymerase Chain Reaction - methods Quality control Rats Species Specificity
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