Effects of Cyclosporine A and Bronchial Transection on Mucociliary Transport in Rats
Background Posttransplant infection remains the leading cause of morbidity and mortality after lung transplantation. We hypothesized that bronchial transection and immunosuppression by cyclosporine both play a key role in the impairment of airway mucociliary clearance, a basic defense system. Method...
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Veröffentlicht in: | The Annals of thoracic surgery 2008-06, Vol.85 (6), p.1925-1929 |
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creator | Pazetti, Rogerio, PhD Pêgo-Fernandes, Paulo M., MD, PhD Lorenzi-Filho, Geraldo, MD, PhD Saldiva, Paulo H.N., MD, PhD Moreira, Luiz Felipe P., MD, PhD Jatene, Fabio B., MD, PhD |
description | Background Posttransplant infection remains the leading cause of morbidity and mortality after lung transplantation. We hypothesized that bronchial transection and immunosuppression by cyclosporine both play a key role in the impairment of airway mucociliary clearance, a basic defense system. Methods Sixty-four rats were assigned to four groups of 16 each according to surgical procedure and drug therapy as follows: sham-operated and saline solution; bronchial transection and saline solution; sham-operated and cyclosporine; bronchial transection and cyclosporine (10 mg/kg/day). Eight animals from each group were euthanized on postoperative day 30 or 90. In vitro mucus transportability, in situ mucociliary transport, and ciliary beating frequency were measured. Results There was a significant impairment ( p < 0.001) on ciliary beating frequency due to either bronchial transection or cyclosporine therapy. In vitro transportability was impaired only in cyclosporine-treated groups ( p < 0.001). In situ mucociliary transport was reduced in cyclosporine-treated animals as well as in those that underwent bronchial transection ( p < 0.001). This impairment was significantly recovered 90 days after operation. In contrast, the effects of cyclosporine did not change over 90 days of treatment. Conclusions These results support our hypothesis that mucociliary clearance is impaired after bronchial transection and cyclosporine therapy. Further studies are necessary to relate this finding with posttransplant infection and also to test some drugs aiming to protect airway mucociliary system. |
doi_str_mv | 10.1016/j.athoracsur.2008.02.084 |
format | Article |
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We hypothesized that bronchial transection and immunosuppression by cyclosporine both play a key role in the impairment of airway mucociliary clearance, a basic defense system. Methods Sixty-four rats were assigned to four groups of 16 each according to surgical procedure and drug therapy as follows: sham-operated and saline solution; bronchial transection and saline solution; sham-operated and cyclosporine; bronchial transection and cyclosporine (10 mg/kg/day). Eight animals from each group were euthanized on postoperative day 30 or 90. In vitro mucus transportability, in situ mucociliary transport, and ciliary beating frequency were measured. Results There was a significant impairment ( p < 0.001) on ciliary beating frequency due to either bronchial transection or cyclosporine therapy. In vitro transportability was impaired only in cyclosporine-treated groups ( p < 0.001). In situ mucociliary transport was reduced in cyclosporine-treated animals as well as in those that underwent bronchial transection ( p < 0.001). This impairment was significantly recovered 90 days after operation. In contrast, the effects of cyclosporine did not change over 90 days of treatment. Conclusions These results support our hypothesis that mucociliary clearance is impaired after bronchial transection and cyclosporine therapy. Further studies are necessary to relate this finding with posttransplant infection and also to test some drugs aiming to protect airway mucociliary system.</description><identifier>ISSN: 0003-4975</identifier><identifier>EISSN: 1552-6259</identifier><identifier>DOI: 10.1016/j.athoracsur.2008.02.084</identifier><identifier>PMID: 18498796</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Bronchi - surgery ; Cardiothoracic Surgery ; Cyclosporine - pharmacology ; Immunosuppressive Agents - pharmacology ; In Vitro Techniques ; Injections, Subcutaneous ; Male ; Mucociliary Clearance - drug effects ; Rats ; Rats, Wistar ; Surgery</subject><ispartof>The Annals of thoracic surgery, 2008-06, Vol.85 (6), p.1925-1929</ispartof><rights>The Society of Thoracic Surgeons</rights><rights>2008 The Society of Thoracic Surgeons</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-5d978c489eb2f3d76081950768fb331b491cc44fe124b5287e68177f730a74633</citedby><cites>FETCH-LOGICAL-c463t-5d978c489eb2f3d76081950768fb331b491cc44fe124b5287e68177f730a74633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18498796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pazetti, Rogerio, PhD</creatorcontrib><creatorcontrib>Pêgo-Fernandes, Paulo M., MD, PhD</creatorcontrib><creatorcontrib>Lorenzi-Filho, Geraldo, MD, PhD</creatorcontrib><creatorcontrib>Saldiva, Paulo H.N., MD, PhD</creatorcontrib><creatorcontrib>Moreira, Luiz Felipe P., MD, PhD</creatorcontrib><creatorcontrib>Jatene, Fabio B., MD, PhD</creatorcontrib><title>Effects of Cyclosporine A and Bronchial Transection on Mucociliary Transport in Rats</title><title>The Annals of thoracic surgery</title><addtitle>Ann Thorac Surg</addtitle><description>Background Posttransplant infection remains the leading cause of morbidity and mortality after lung transplantation. We hypothesized that bronchial transection and immunosuppression by cyclosporine both play a key role in the impairment of airway mucociliary clearance, a basic defense system. Methods Sixty-four rats were assigned to four groups of 16 each according to surgical procedure and drug therapy as follows: sham-operated and saline solution; bronchial transection and saline solution; sham-operated and cyclosporine; bronchial transection and cyclosporine (10 mg/kg/day). Eight animals from each group were euthanized on postoperative day 30 or 90. In vitro mucus transportability, in situ mucociliary transport, and ciliary beating frequency were measured. Results There was a significant impairment ( p < 0.001) on ciliary beating frequency due to either bronchial transection or cyclosporine therapy. In vitro transportability was impaired only in cyclosporine-treated groups ( p < 0.001). In situ mucociliary transport was reduced in cyclosporine-treated animals as well as in those that underwent bronchial transection ( p < 0.001). This impairment was significantly recovered 90 days after operation. In contrast, the effects of cyclosporine did not change over 90 days of treatment. Conclusions These results support our hypothesis that mucociliary clearance is impaired after bronchial transection and cyclosporine therapy. Further studies are necessary to relate this finding with posttransplant infection and also to test some drugs aiming to protect airway mucociliary system.</description><subject>Animals</subject><subject>Bronchi - surgery</subject><subject>Cardiothoracic Surgery</subject><subject>Cyclosporine - pharmacology</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Injections, Subcutaneous</subject><subject>Male</subject><subject>Mucociliary Clearance - drug effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Surgery</subject><issn>0003-4975</issn><issn>1552-6259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFr3DAQhUVpSDZp_kLRqTe7I1m25EshWdI2kBJot2chyyOirVfaSnZh_3217EKgp4JAiHnvzegbQiiDmgHrPm5rM7_EZGxeUs0BVA28BiXekBVrW151vO3fkhUANJXoZXtFrnPelicv5UtyxZToley7Fdk8OId2zjQ6uj7YKeZ9TD4gvaMmjPQ-xWBfvJnoJpmQi9LHQMv5ttho_eRNOpxKxTZTH-h3M-d35MKZKePt-b4hPz8_bNZfq6fnL4_ru6fKiq6Zq3bspbJC9Thw14yyA8X6FmSn3NA0bBA9s1YIh4yLoeVKYqeYlE42YGRJaG7Ih1PuPsXfC-ZZ73y2OE0mYFyyliWLKaWKUJ2ENsWcEzq9T35XZtcM9JGo3upXovpIVAPXhWixvj_3WIYdjq_GM8IiuD8JsPz0j8eks_UYLI4-FV56jP5_unz6J8ROPnhrpl94wLyNSwqFpGY6F4P-cdzscbGgAETh1vwFlxGg4w</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Pazetti, Rogerio, PhD</creator><creator>Pêgo-Fernandes, Paulo M., MD, PhD</creator><creator>Lorenzi-Filho, Geraldo, MD, PhD</creator><creator>Saldiva, Paulo H.N., MD, PhD</creator><creator>Moreira, Luiz Felipe P., MD, PhD</creator><creator>Jatene, Fabio B., MD, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080601</creationdate><title>Effects of Cyclosporine A and Bronchial Transection on Mucociliary Transport in Rats</title><author>Pazetti, Rogerio, PhD ; Pêgo-Fernandes, Paulo M., MD, PhD ; Lorenzi-Filho, Geraldo, MD, PhD ; Saldiva, Paulo H.N., MD, PhD ; Moreira, Luiz Felipe P., MD, PhD ; Jatene, Fabio B., MD, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-5d978c489eb2f3d76081950768fb331b491cc44fe124b5287e68177f730a74633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Bronchi - surgery</topic><topic>Cardiothoracic Surgery</topic><topic>Cyclosporine - pharmacology</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Injections, Subcutaneous</topic><topic>Male</topic><topic>Mucociliary Clearance - drug effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pazetti, Rogerio, PhD</creatorcontrib><creatorcontrib>Pêgo-Fernandes, Paulo M., MD, PhD</creatorcontrib><creatorcontrib>Lorenzi-Filho, Geraldo, MD, PhD</creatorcontrib><creatorcontrib>Saldiva, Paulo H.N., MD, PhD</creatorcontrib><creatorcontrib>Moreira, Luiz Felipe P., MD, PhD</creatorcontrib><creatorcontrib>Jatene, Fabio B., MD, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Annals of thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pazetti, Rogerio, PhD</au><au>Pêgo-Fernandes, Paulo M., MD, PhD</au><au>Lorenzi-Filho, Geraldo, MD, PhD</au><au>Saldiva, Paulo H.N., MD, PhD</au><au>Moreira, Luiz Felipe P., MD, PhD</au><au>Jatene, Fabio B., MD, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Cyclosporine A and Bronchial Transection on Mucociliary Transport in Rats</atitle><jtitle>The Annals of thoracic surgery</jtitle><addtitle>Ann Thorac Surg</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>85</volume><issue>6</issue><spage>1925</spage><epage>1929</epage><pages>1925-1929</pages><issn>0003-4975</issn><eissn>1552-6259</eissn><abstract>Background Posttransplant infection remains the leading cause of morbidity and mortality after lung transplantation. We hypothesized that bronchial transection and immunosuppression by cyclosporine both play a key role in the impairment of airway mucociliary clearance, a basic defense system. Methods Sixty-four rats were assigned to four groups of 16 each according to surgical procedure and drug therapy as follows: sham-operated and saline solution; bronchial transection and saline solution; sham-operated and cyclosporine; bronchial transection and cyclosporine (10 mg/kg/day). Eight animals from each group were euthanized on postoperative day 30 or 90. In vitro mucus transportability, in situ mucociliary transport, and ciliary beating frequency were measured. Results There was a significant impairment ( p < 0.001) on ciliary beating frequency due to either bronchial transection or cyclosporine therapy. In vitro transportability was impaired only in cyclosporine-treated groups ( p < 0.001). In situ mucociliary transport was reduced in cyclosporine-treated animals as well as in those that underwent bronchial transection ( p < 0.001). This impairment was significantly recovered 90 days after operation. In contrast, the effects of cyclosporine did not change over 90 days of treatment. Conclusions These results support our hypothesis that mucociliary clearance is impaired after bronchial transection and cyclosporine therapy. Further studies are necessary to relate this finding with posttransplant infection and also to test some drugs aiming to protect airway mucociliary system.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>18498796</pmid><doi>10.1016/j.athoracsur.2008.02.084</doi><tpages>5</tpages></addata></record> |
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subjects | Animals Bronchi - surgery Cardiothoracic Surgery Cyclosporine - pharmacology Immunosuppressive Agents - pharmacology In Vitro Techniques Injections, Subcutaneous Male Mucociliary Clearance - drug effects Rats Rats, Wistar Surgery |
title | Effects of Cyclosporine A and Bronchial Transection on Mucociliary Transport in Rats |
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