Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein
The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long...
Gespeichert in:
| Veröffentlicht in: | Parasitology research (1987) 2007-08, Vol.101 (3), p.737-742 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 742 |
|---|---|
| container_issue | 3 |
| container_start_page | 737 |
| container_title | Parasitology research (1987) |
| container_volume | 101 |
| creator | Zhou, Zhenwen Hu, Xuchu Huang, Yan Hu, Huixia Ma, Changling Chen, Xiaoxiang Hu, Fengyu Xu, Jin Lu, Fangli Wu, Zhongdao Yu, Xinbing |
| description | The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kDa protein with an isoelectric point of 4.33. The deduced amino acid sequence exhibits 40 and 37% identity to Schistosoma japonicum sj20.8 and Schistosoma mansoni Sm 20.8, respectively. TP20.8 transcripts were detected in the adult worm and metacercariae cDNA libraries of C. sinensis but not in the egg. Recombinant C. sinensis TP20.8 protein was produced and purified from Escherichia coli BL21. Using specific anti-recombinant TP20.8 protein sera, the TP20.8 protein was immunohistochemically localized to the outer-surface membrane of C. sinensis. The specificity and sensitivity of the recombinant antigen for serologic diagnosis was assessed by enzyme-linked immunosorbent assay using serum from 100 patients with clonorchiasis, 20 patients with schistosomiasis, and 30 negative controls. The sensitivity was 68%, and the specificity was 84%. The antigen was less useful for the serodiagnosis of clonorchiasis with IgG. |
| doi_str_mv | 10.1007/s00436-007-0541-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70760482</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19882639</sourcerecordid><originalsourceid>FETCH-LOGICAL-c450t-42e17f823802fbc3f654140fc54b3856506f2a7d9643ac5f7a3f21f57b686a863</originalsourceid><addsrcrecordid>eNqFkTtvFDEUhS0EIkvgB9CAG-gmXI-fU6IVLykRRUht3fXYi5HXDvYMUv49XnallMiFT_Gdc699CHnN4IoB6A8NQHA1dDmAFGwwT8iGCT4ObJLyKdnA1DUwxi_Ii9Z-ATCthHhOLpgWWkkOGxJuSvJuTVipSyXHvKeYZxpnn5cYosMllkxLoEhz-eMT3XaqVPczNtpi9rl1sffZU59dmf_56eL366EHYKL3tSw-5pfkWcDU_KvzfUnuPn_6sf06XH__8m378XpwQsIyiNEzHczIDYxh53hQ_VkCgpNix41UElQYUc-TEhydDBp5GFmQeqeMQqP4JXl_yu1zf6--LfYQm_MpYfZlbVaDViD6gP-BbDJmVHzqIDuBrpbWqg_2vsYD1gfLwB5bsKcW7FEeW7Cme96cw9fdwc-PjvO3d-DdGcDmMIWK2cX2yJlJ9HPc8u2JC1gs7mtn7m5HYLwPm0BJzf8CS_eYxw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19882639</pqid></control><display><type>article</type><title>Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Zhou, Zhenwen ; Hu, Xuchu ; Huang, Yan ; Hu, Huixia ; Ma, Changling ; Chen, Xiaoxiang ; Hu, Fengyu ; Xu, Jin ; Lu, Fangli ; Wu, Zhongdao ; Yu, Xinbing</creator><creatorcontrib>Zhou, Zhenwen ; Hu, Xuchu ; Huang, Yan ; Hu, Huixia ; Ma, Changling ; Chen, Xiaoxiang ; Hu, Fengyu ; Xu, Jin ; Lu, Fangli ; Wu, Zhongdao ; Yu, Xinbing</creatorcontrib><description>The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kDa protein with an isoelectric point of 4.33. The deduced amino acid sequence exhibits 40 and 37% identity to Schistosoma japonicum sj20.8 and Schistosoma mansoni Sm 20.8, respectively. TP20.8 transcripts were detected in the adult worm and metacercariae cDNA libraries of C. sinensis but not in the egg. Recombinant C. sinensis TP20.8 protein was produced and purified from Escherichia coli BL21. Using specific anti-recombinant TP20.8 protein sera, the TP20.8 protein was immunohistochemically localized to the outer-surface membrane of C. sinensis. The specificity and sensitivity of the recombinant antigen for serologic diagnosis was assessed by enzyme-linked immunosorbent assay using serum from 100 patients with clonorchiasis, 20 patients with schistosomiasis, and 30 negative controls. The sensitivity was 68%, and the specificity was 84%. The antigen was less useful for the serodiagnosis of clonorchiasis with IgG.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-007-0541-8</identifier><identifier>PMID: 17476530</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Animals ; Antibodies, Helminth - blood ; Antigens, Helminth - chemistry ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Antigens, Helminth - metabolism ; Biological and medical sciences ; Cats ; Cloning, Molecular ; Clonorchiasis - diagnosis ; Clonorchiasis - parasitology ; Clonorchis ; Clonorchis sinensis ; Clonorchis sinensis - genetics ; Clonorchis sinensis - immunology ; Clonorchis sinensis - metabolism ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Gene Library ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Helminth Proteins - chemistry ; Helminth Proteins - genetics ; Helminth Proteins - immunology ; Helminth Proteins - metabolism ; Humans ; Immunoglobulin G - blood ; Invertebrates ; Membrane Proteins - chemistry ; Membrane Proteins - genetics ; Membrane Proteins - immunology ; Membrane Proteins - metabolism ; Molecular Sequence Data ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinant Proteins - metabolism ; Schistosoma japonicum ; Schistosoma mansoni ; Sensitivity and Specificity ; Sequence Analysis, DNA</subject><ispartof>Parasitology research (1987), 2007-08, Vol.101 (3), p.737-742</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-42e17f823802fbc3f654140fc54b3856506f2a7d9643ac5f7a3f21f57b686a863</citedby><cites>FETCH-LOGICAL-c450t-42e17f823802fbc3f654140fc54b3856506f2a7d9643ac5f7a3f21f57b686a863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18949492$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17476530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Zhenwen</creatorcontrib><creatorcontrib>Hu, Xuchu</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Hu, Huixia</creatorcontrib><creatorcontrib>Ma, Changling</creatorcontrib><creatorcontrib>Chen, Xiaoxiang</creatorcontrib><creatorcontrib>Hu, Fengyu</creatorcontrib><creatorcontrib>Xu, Jin</creatorcontrib><creatorcontrib>Lu, Fangli</creatorcontrib><creatorcontrib>Wu, Zhongdao</creatorcontrib><creatorcontrib>Yu, Xinbing</creatorcontrib><title>Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><description>The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kDa protein with an isoelectric point of 4.33. The deduced amino acid sequence exhibits 40 and 37% identity to Schistosoma japonicum sj20.8 and Schistosoma mansoni Sm 20.8, respectively. TP20.8 transcripts were detected in the adult worm and metacercariae cDNA libraries of C. sinensis but not in the egg. Recombinant C. sinensis TP20.8 protein was produced and purified from Escherichia coli BL21. Using specific anti-recombinant TP20.8 protein sera, the TP20.8 protein was immunohistochemically localized to the outer-surface membrane of C. sinensis. The specificity and sensitivity of the recombinant antigen for serologic diagnosis was assessed by enzyme-linked immunosorbent assay using serum from 100 patients with clonorchiasis, 20 patients with schistosomiasis, and 30 negative controls. The sensitivity was 68%, and the specificity was 84%. The antigen was less useful for the serodiagnosis of clonorchiasis with IgG.</description><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antigens, Helminth - chemistry</subject><subject>Antigens, Helminth - genetics</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cats</subject><subject>Cloning, Molecular</subject><subject>Clonorchiasis - diagnosis</subject><subject>Clonorchiasis - parasitology</subject><subject>Clonorchis</subject><subject>Clonorchis sinensis</subject><subject>Clonorchis sinensis - genetics</subject><subject>Clonorchis sinensis - immunology</subject><subject>Clonorchis sinensis - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Helminth Proteins - chemistry</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - immunology</subject><subject>Helminth Proteins - metabolism</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Invertebrates</subject><subject>Membrane Proteins - chemistry</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - immunology</subject><subject>Membrane Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - metabolism</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma mansoni</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Analysis, DNA</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtvFDEUhS0EIkvgB9CAG-gmXI-fU6IVLykRRUht3fXYi5HXDvYMUv49XnallMiFT_Gdc699CHnN4IoB6A8NQHA1dDmAFGwwT8iGCT4ObJLyKdnA1DUwxi_Ii9Z-ATCthHhOLpgWWkkOGxJuSvJuTVipSyXHvKeYZxpnn5cYosMllkxLoEhz-eMT3XaqVPczNtpi9rl1sffZU59dmf_56eL366EHYKL3tSw-5pfkWcDU_KvzfUnuPn_6sf06XH__8m378XpwQsIyiNEzHczIDYxh53hQ_VkCgpNix41UElQYUc-TEhydDBp5GFmQeqeMQqP4JXl_yu1zf6--LfYQm_MpYfZlbVaDViD6gP-BbDJmVHzqIDuBrpbWqg_2vsYD1gfLwB5bsKcW7FEeW7Cme96cw9fdwc-PjvO3d-DdGcDmMIWK2cX2yJlJ9HPc8u2JC1gs7mtn7m5HYLwPm0BJzf8CS_eYxw</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Zhou, Zhenwen</creator><creator>Hu, Xuchu</creator><creator>Huang, Yan</creator><creator>Hu, Huixia</creator><creator>Ma, Changling</creator><creator>Chen, Xiaoxiang</creator><creator>Hu, Fengyu</creator><creator>Xu, Jin</creator><creator>Lu, Fangli</creator><creator>Wu, Zhongdao</creator><creator>Yu, Xinbing</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070801</creationdate><title>Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein</title><author>Zhou, Zhenwen ; Hu, Xuchu ; Huang, Yan ; Hu, Huixia ; Ma, Changling ; Chen, Xiaoxiang ; Hu, Fengyu ; Xu, Jin ; Lu, Fangli ; Wu, Zhongdao ; Yu, Xinbing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-42e17f823802fbc3f654140fc54b3856506f2a7d9643ac5f7a3f21f57b686a863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antigens, Helminth - chemistry</topic><topic>Antigens, Helminth - genetics</topic><topic>Antigens, Helminth - immunology</topic><topic>Antigens, Helminth - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cats</topic><topic>Cloning, Molecular</topic><topic>Clonorchiasis - diagnosis</topic><topic>Clonorchiasis - parasitology</topic><topic>Clonorchis</topic><topic>Clonorchis sinensis</topic><topic>Clonorchis sinensis - genetics</topic><topic>Clonorchis sinensis - immunology</topic><topic>Clonorchis sinensis - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Helminth Proteins - chemistry</topic><topic>Helminth Proteins - genetics</topic><topic>Helminth Proteins - immunology</topic><topic>Helminth Proteins - metabolism</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Invertebrates</topic><topic>Membrane Proteins - chemistry</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - immunology</topic><topic>Membrane Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - metabolism</topic><topic>Schistosoma japonicum</topic><topic>Schistosoma mansoni</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Zhenwen</creatorcontrib><creatorcontrib>Hu, Xuchu</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Hu, Huixia</creatorcontrib><creatorcontrib>Ma, Changling</creatorcontrib><creatorcontrib>Chen, Xiaoxiang</creatorcontrib><creatorcontrib>Hu, Fengyu</creatorcontrib><creatorcontrib>Xu, Jin</creatorcontrib><creatorcontrib>Lu, Fangli</creatorcontrib><creatorcontrib>Wu, Zhongdao</creatorcontrib><creatorcontrib>Yu, Xinbing</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Zhenwen</au><au>Hu, Xuchu</au><au>Huang, Yan</au><au>Hu, Huixia</au><au>Ma, Changling</au><au>Chen, Xiaoxiang</au><au>Hu, Fengyu</au><au>Xu, Jin</au><au>Lu, Fangli</au><au>Wu, Zhongdao</au><au>Yu, Xinbing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein</atitle><jtitle>Parasitology research (1987)</jtitle><addtitle>Parasitol Res</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>101</volume><issue>3</issue><spage>737</spage><epage>742</epage><pages>737-742</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A cDNA encoding a novel tegumental protein 20.8 kDa (TP20.8) was found by large-scale sequencing of a Clonorchis sinensis cDNA library. This new cDNA was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kDa protein with an isoelectric point of 4.33. The deduced amino acid sequence exhibits 40 and 37% identity to Schistosoma japonicum sj20.8 and Schistosoma mansoni Sm 20.8, respectively. TP20.8 transcripts were detected in the adult worm and metacercariae cDNA libraries of C. sinensis but not in the egg. Recombinant C. sinensis TP20.8 protein was produced and purified from Escherichia coli BL21. Using specific anti-recombinant TP20.8 protein sera, the TP20.8 protein was immunohistochemically localized to the outer-surface membrane of C. sinensis. The specificity and sensitivity of the recombinant antigen for serologic diagnosis was assessed by enzyme-linked immunosorbent assay using serum from 100 patients with clonorchiasis, 20 patients with schistosomiasis, and 30 negative controls. The sensitivity was 68%, and the specificity was 84%. The antigen was less useful for the serodiagnosis of clonorchiasis with IgG.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17476530</pmid><doi>10.1007/s00436-007-0541-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2007-08, Vol.101 (3), p.737-742 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70760482 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Antibodies, Helminth - blood Antigens, Helminth - chemistry Antigens, Helminth - genetics Antigens, Helminth - immunology Antigens, Helminth - metabolism Biological and medical sciences Cats Cloning, Molecular Clonorchiasis - diagnosis Clonorchiasis - parasitology Clonorchis Clonorchis sinensis Clonorchis sinensis - genetics Clonorchis sinensis - immunology Clonorchis sinensis - metabolism Enzyme-Linked Immunosorbent Assay Escherichia coli Fundamental and applied biological sciences. Psychology Gene Library General aspects General aspects and techniques. Study of several systematic groups. Models Helminth Proteins - chemistry Helminth Proteins - genetics Helminth Proteins - immunology Helminth Proteins - metabolism Humans Immunoglobulin G - blood Invertebrates Membrane Proteins - chemistry Membrane Proteins - genetics Membrane Proteins - immunology Membrane Proteins - metabolism Molecular Sequence Data Rats Rats, Sprague-Dawley Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - metabolism Schistosoma japonicum Schistosoma mansoni Sensitivity and Specificity Sequence Analysis, DNA |
| title | Molecular cloning and identification of a novel Clonorchis sinensis gene encoding a tegumental protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T19%3A19%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20cloning%20and%20identification%20of%20a%20novel%20Clonorchis%20sinensis%20gene%20encoding%20a%20tegumental%20protein&rft.jtitle=Parasitology%20research%20(1987)&rft.au=Zhou,%20Zhenwen&rft.date=2007-08-01&rft.volume=101&rft.issue=3&rft.spage=737&rft.epage=742&rft.pages=737-742&rft.issn=0932-0113&rft.eissn=1432-1955&rft.coden=PARREZ&rft_id=info:doi/10.1007/s00436-007-0541-8&rft_dat=%3Cproquest_cross%3E19882639%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19882639&rft_id=info:pmid/17476530&rfr_iscdi=true |