Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-γ and antibody responses in mice
Abstract Falciparum malaria vaccine candidates have been developed using recombinant, replication-deficient serotype 5 and 35 adenoviruses (Ad5, Ad35) encoding the Plasmodium falciparum circumsporozoite surface protein (CSP) (Ad5.CS, Ad35.CS) (Crucell Holland BV, Leiden, The Netherlands). To evaluat...
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| Veröffentlicht in: | Vaccine 2008-06, Vol.26 (23), p.2818-2823 |
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| creator | Shott, Joseph P McGrath, Shannon M Pau, Maria Grazia Custers, Jerome H.V Ophorst, Olga Demoitié, Marie-Ange Dubois, Marie-Claude Komisar, Jack Cobb, Michelle Kester, Kent E Dubois, Patrice Cohen, Joe Goudsmit, Jaap Heppner, D. Gray Stewart, V. Ann |
| description | Abstract Falciparum malaria vaccine candidates have been developed using recombinant, replication-deficient serotype 5 and 35 adenoviruses (Ad5, Ad35) encoding the Plasmodium falciparum circumsporozoite surface protein (CSP) (Ad5.CS, Ad35.CS) (Crucell Holland BV, Leiden, The Netherlands). To evaluate the immunogenicity of these constructs, BALB/cJ mice were immunized twice with either Ad5.CS, Ad35.CS, empty Ad5-vector (eAd5), empty Ad35 vector (eAd35), or saline. Another group received the CSP-based RTS,S malaria vaccine formulated in the proprietary Adjuvant System AS01B (GlaxoSmithKline Biologicals, Rixensart, Belgium). Here we report that Ad5.CS, Ad35.CS, and RTS,S/AS01B, elicited both cellular and serologic CSP antigen-specific responses in mice. These adenoviral vectors induce strong malaria-specific immunity and warrant further evaluation. |
| doi_str_mv | 10.1016/j.vaccine.2008.03.080 |
| format | Article |
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Gray ; Stewart, V. Ann</creator><creatorcontrib>Shott, Joseph P ; McGrath, Shannon M ; Pau, Maria Grazia ; Custers, Jerome H.V ; Ophorst, Olga ; Demoitié, Marie-Ange ; Dubois, Marie-Claude ; Komisar, Jack ; Cobb, Michelle ; Kester, Kent E ; Dubois, Patrice ; Cohen, Joe ; Goudsmit, Jaap ; Heppner, D. Gray ; Stewart, V. Ann</creatorcontrib><description>Abstract Falciparum malaria vaccine candidates have been developed using recombinant, replication-deficient serotype 5 and 35 adenoviruses (Ad5, Ad35) encoding the Plasmodium falciparum circumsporozoite surface protein (CSP) (Ad5.CS, Ad35.CS) (Crucell Holland BV, Leiden, The Netherlands). To evaluate the immunogenicity of these constructs, BALB/cJ mice were immunized twice with either Ad5.CS, Ad35.CS, empty Ad5-vector (eAd5), empty Ad35 vector (eAd35), or saline. Another group received the CSP-based RTS,S malaria vaccine formulated in the proprietary Adjuvant System AS01B (GlaxoSmithKline Biologicals, Rixensart, Belgium). Here we report that Ad5.CS, Ad35.CS, and RTS,S/AS01B, elicited both cellular and serologic CSP antigen-specific responses in mice. These adenoviral vectors induce strong malaria-specific immunity and warrant further evaluation.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2008.03.080</identifier><identifier>PMID: 18455276</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adenoviridae - genetics ; Adenovirus 35 ; Adenovirus 5 ; Allergy and Immunology ; Animals ; Antibodies, Protozoan - analysis ; Antibodies, Protozoan - biosynthesis ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Applied microbiology ; AS01B ; Biological and medical sciences ; Circumsporozoite ; Enzyme-Linked Immunosorbent Assay ; Falciparum ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors ; Human protozoal diseases ; Immunization Schedule ; Immunization, Secondary ; Infectious diseases ; Interferon-gamma - biosynthesis ; Life cycle. Host-agent relationship. Pathogenesis ; Malaria ; Malaria Vaccines - immunology ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Parasitic diseases ; Plasmodium falciparum - genetics ; Plasmodium falciparum - metabolism ; Protozoa ; Protozoal diseases ; Sporozoites - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Virology</subject><ispartof>Vaccine, 2008-06, Vol.26 (23), p.2818-2823</ispartof><rights>2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-61f3e5bf192bc1f37a45f0f210c5526be6bb943d58856980d2e39c38f9a526373</citedby><cites>FETCH-LOGICAL-c448t-61f3e5bf192bc1f37a45f0f210c5526be6bb943d58856980d2e39c38f9a526373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vaccine.2008.03.080$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002,64394</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20359357$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18455276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shott, Joseph P</creatorcontrib><creatorcontrib>McGrath, Shannon M</creatorcontrib><creatorcontrib>Pau, Maria Grazia</creatorcontrib><creatorcontrib>Custers, Jerome H.V</creatorcontrib><creatorcontrib>Ophorst, Olga</creatorcontrib><creatorcontrib>Demoitié, Marie-Ange</creatorcontrib><creatorcontrib>Dubois, Marie-Claude</creatorcontrib><creatorcontrib>Komisar, Jack</creatorcontrib><creatorcontrib>Cobb, Michelle</creatorcontrib><creatorcontrib>Kester, Kent E</creatorcontrib><creatorcontrib>Dubois, Patrice</creatorcontrib><creatorcontrib>Cohen, Joe</creatorcontrib><creatorcontrib>Goudsmit, Jaap</creatorcontrib><creatorcontrib>Heppner, D. Gray</creatorcontrib><creatorcontrib>Stewart, V. Ann</creatorcontrib><title>Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-γ and antibody responses in mice</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Falciparum malaria vaccine candidates have been developed using recombinant, replication-deficient serotype 5 and 35 adenoviruses (Ad5, Ad35) encoding the Plasmodium falciparum circumsporozoite surface protein (CSP) (Ad5.CS, Ad35.CS) (Crucell Holland BV, Leiden, The Netherlands). To evaluate the immunogenicity of these constructs, BALB/cJ mice were immunized twice with either Ad5.CS, Ad35.CS, empty Ad5-vector (eAd5), empty Ad35 vector (eAd35), or saline. Another group received the CSP-based RTS,S malaria vaccine formulated in the proprietary Adjuvant System AS01B (GlaxoSmithKline Biologicals, Rixensart, Belgium). Here we report that Ad5.CS, Ad35.CS, and RTS,S/AS01B, elicited both cellular and serologic CSP antigen-specific responses in mice. These adenoviral vectors induce strong malaria-specific immunity and warrant further evaluation.</description><subject>Adenoviridae - genetics</subject><subject>Adenovirus 35</subject><subject>Adenovirus 5</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibodies, Protozoan - analysis</subject><subject>Antibodies, Protozoan - biosynthesis</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Applied microbiology</subject><subject>AS01B</subject><subject>Biological and medical sciences</subject><subject>Circumsporozoite</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Falciparum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors</subject><subject>Human protozoal diseases</subject><subject>Immunization Schedule</subject><subject>Immunization, Secondary</subject><subject>Infectious diseases</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Malaria</subject><subject>Malaria Vaccines - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Parasitic diseases</subject><subject>Plasmodium falciparum - genetics</subject><subject>Plasmodium falciparum - metabolism</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><subject>Sporozoites - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUsGO1DAMrRCIXRY-AZQLe-uQNE2bXkCrFQsrrQAJkLhFaequPLRJidsRwy9x5D_4JlJmBBIXTnHk5_dsP2fZY8E3govq2Xazs86hh03Bud5wueGa38lOha5lXiih72anvKjKvBT800n2gGjLOVdSNPezE6FLpYq6Os2-X3Tgww7jQkwx6zsmFduBm0MkBl-nCETob9m7wdIYOlxG1tvB4WRjCh1Gt4w0hRi-BZyB0RJ764BNMcyAnsGADmc2pZ-fE_2Mt-BzmsBhj445GIZlsJFdX73Jf_74rb-C2tDtWZKegicglohGdPAwu5e0CR4d37Ps49XLD5ev85u3r64vL25yV5Z6zivRS1BtL5qidSmubal63heCuzR01ULVtk0pO6W1qhrNuwJk46TuG5vSspZn2fmBN03xZQGazYi0tmo9hIVMzWtVal0moDoAXQxEEXozRRxt3BvBzeqS2ZqjS2Z1yXBpkkup7slRYGlH6P5WHW1JgKdHgCVnhz5a75D-4AouVSPV2umLAw7SOnYI0ZBD8A46jMlD0wX8byvP_2FwA3pMop9hD7QNS_Rp10YYKgw379eTWi8q1fJSJIJfSXbMYQ</recordid><startdate>20080602</startdate><enddate>20080602</enddate><creator>Shott, Joseph P</creator><creator>McGrath, Shannon M</creator><creator>Pau, Maria Grazia</creator><creator>Custers, Jerome H.V</creator><creator>Ophorst, Olga</creator><creator>Demoitié, Marie-Ange</creator><creator>Dubois, Marie-Claude</creator><creator>Komisar, Jack</creator><creator>Cobb, Michelle</creator><creator>Kester, Kent E</creator><creator>Dubois, Patrice</creator><creator>Cohen, Joe</creator><creator>Goudsmit, Jaap</creator><creator>Heppner, D. Gray</creator><creator>Stewart, V. Ann</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080602</creationdate><title>Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-γ and antibody responses in mice</title><author>Shott, Joseph P ; McGrath, Shannon M ; Pau, Maria Grazia ; Custers, Jerome H.V ; Ophorst, Olga ; Demoitié, Marie-Ange ; Dubois, Marie-Claude ; Komisar, Jack ; Cobb, Michelle ; Kester, Kent E ; Dubois, Patrice ; Cohen, Joe ; Goudsmit, Jaap ; Heppner, D. Gray ; Stewart, V. Ann</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-61f3e5bf192bc1f37a45f0f210c5526be6bb943d58856980d2e39c38f9a526373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adenoviridae - genetics</topic><topic>Adenovirus 35</topic><topic>Adenovirus 5</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibodies, Protozoan - analysis</topic><topic>Antibodies, Protozoan - biosynthesis</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Applied microbiology</topic><topic>AS01B</topic><topic>Biological and medical sciences</topic><topic>Circumsporozoite</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Falciparum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors</topic><topic>Human protozoal diseases</topic><topic>Immunization Schedule</topic><topic>Immunization, Secondary</topic><topic>Infectious diseases</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Malaria</topic><topic>Malaria Vaccines - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Parasitic diseases</topic><topic>Plasmodium falciparum - genetics</topic><topic>Plasmodium falciparum - metabolism</topic><topic>Protozoa</topic><topic>Protozoal diseases</topic><topic>Sporozoites - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shott, Joseph P</creatorcontrib><creatorcontrib>McGrath, Shannon M</creatorcontrib><creatorcontrib>Pau, Maria Grazia</creatorcontrib><creatorcontrib>Custers, Jerome H.V</creatorcontrib><creatorcontrib>Ophorst, Olga</creatorcontrib><creatorcontrib>Demoitié, Marie-Ange</creatorcontrib><creatorcontrib>Dubois, Marie-Claude</creatorcontrib><creatorcontrib>Komisar, Jack</creatorcontrib><creatorcontrib>Cobb, Michelle</creatorcontrib><creatorcontrib>Kester, Kent E</creatorcontrib><creatorcontrib>Dubois, Patrice</creatorcontrib><creatorcontrib>Cohen, Joe</creatorcontrib><creatorcontrib>Goudsmit, Jaap</creatorcontrib><creatorcontrib>Heppner, D. 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Ann</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-γ and antibody responses in mice</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2008-06-02</date><risdate>2008</risdate><volume>26</volume><issue>23</issue><spage>2818</spage><epage>2823</epage><pages>2818-2823</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Falciparum malaria vaccine candidates have been developed using recombinant, replication-deficient serotype 5 and 35 adenoviruses (Ad5, Ad35) encoding the Plasmodium falciparum circumsporozoite surface protein (CSP) (Ad5.CS, Ad35.CS) (Crucell Holland BV, Leiden, The Netherlands). To evaluate the immunogenicity of these constructs, BALB/cJ mice were immunized twice with either Ad5.CS, Ad35.CS, empty Ad5-vector (eAd5), empty Ad35 vector (eAd35), or saline. Another group received the CSP-based RTS,S malaria vaccine formulated in the proprietary Adjuvant System AS01B (GlaxoSmithKline Biologicals, Rixensart, Belgium). Here we report that Ad5.CS, Ad35.CS, and RTS,S/AS01B, elicited both cellular and serologic CSP antigen-specific responses in mice. These adenoviral vectors induce strong malaria-specific immunity and warrant further evaluation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18455276</pmid><doi>10.1016/j.vaccine.2008.03.080</doi><tpages>6</tpages></addata></record> |
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| ispartof | Vaccine, 2008-06, Vol.26 (23), p.2818-2823 |
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| subjects | Adenoviridae - genetics Adenovirus 35 Adenovirus 5 Allergy and Immunology Animals Antibodies, Protozoan - analysis Antibodies, Protozoan - biosynthesis Antigens, Protozoan - genetics Antigens, Protozoan - immunology Applied microbiology AS01B Biological and medical sciences Circumsporozoite Enzyme-Linked Immunosorbent Assay Falciparum Fundamental and applied biological sciences. Psychology Genetic Vectors Human protozoal diseases Immunization Schedule Immunization, Secondary Infectious diseases Interferon-gamma - biosynthesis Life cycle. Host-agent relationship. Pathogenesis Malaria Malaria Vaccines - immunology Male Medical sciences Mice Mice, Inbred BALB C Microbiology Miscellaneous Parasitic diseases Plasmodium falciparum - genetics Plasmodium falciparum - metabolism Protozoa Protozoal diseases Sporozoites - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology |
| title | Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-γ and antibody responses in mice |
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