Isolation of signal transduction complexes using biotin and crosslinking methodologies
We have developed a strategy to preferentially label the N‐terminal α‐amino groups of intact proteins allowing the internal ϵ‐amino groups to remain free to react with chemical crosslinking reagents. The convergence of these methodologies allows biotinylated ligands to bind to their receptors within...
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| Veröffentlicht in: | Proteomics (Weinheim) 2007-07, Vol.7 (14), p.2371-2374 |
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| container_issue | 14 |
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| container_title | Proteomics (Weinheim) |
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| creator | Freed, Julie K. Smith, Julia R. Li, Peigang Greene, Andrew S. |
| description | We have developed a strategy to preferentially label the N‐terminal α‐amino groups of intact proteins allowing the internal ϵ‐amino groups to remain free to react with chemical crosslinking reagents. The convergence of these methodologies allows biotinylated ligands to bind to their receptors within the cell membrane followed by removal of the crosslinked complex from cell lysate. This technique allows for the isolation of protein complexes in an MS‐compatible system, thus providing a tool for furthering our understanding of signal transduction. |
| doi_str_mv | 10.1002/pmic.200700219 |
| format | Article |
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Psychology</subject><subject>Miscellaneous</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Rats</subject><subject>Receptors, Tumor Necrosis Factor - metabolism</subject><subject>Signal Transduction</subject><subject>Signaling</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>TNF-alpha</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1P3DAQxa2qqMC21x6rXNpbFtv5sH1EC4VFC-2BFomL5TiTrcGJl0wi4L_Hy64Wbj1Y43n6vZnRI-Qro1NGKT9atc5OOaUiNkx9IAesZEWqZMk-7v5Ftk8OEe8oZUIq8YnsM1HyjCtxQP7OMXgzuNAloUnQLTvjk6E3HdajfZVtaFcengCTEV23TCoXBtclpqsT2wdE77r7td7C8C_UwYelA_xM9hrjEb5s64T8-Xl6PTtPF7_O5rPjRWpzxlQqS6CGK2gyWZZVk2cst7QqG1YLDrKQoKKc87ypARTPclnFojJemXUX34T82Mxd9eFhBBx069CC96aDMKIWVBRM5iyC0w34enMPjV71rjX9s2ZUr5PU6yT1Lslo-LadPFYt1G_4NroIfN8CBq3xTczMOnzjpKKyYEXk1IZ7dB6e_7NW_76cz94fkW68Dgd42nlNf69LkYlC31yd6avzk9vbC3mtb7IXVRidXg</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Freed, Julie K.</creator><creator>Smith, Julia R.</creator><creator>Li, Peigang</creator><creator>Greene, Andrew S.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070701</creationdate><title>Isolation of signal transduction complexes using biotin and crosslinking methodologies</title><author>Freed, Julie K. ; Smith, Julia R. ; Li, Peigang ; Greene, Andrew S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4119-86e0a29ef3866bf4314c0b6f1d72e858e966b424fdee92348be92932ba9234923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotin</topic><topic>Biotin - chemistry</topic><topic>Cross-Linking Reagents - chemistry</topic><topic>Crosslinking</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Miscellaneous</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Rats</topic><topic>Receptors, Tumor Necrosis Factor - metabolism</topic><topic>Signal Transduction</topic><topic>Signaling</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>TNF-alpha</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freed, Julie K.</creatorcontrib><creatorcontrib>Smith, Julia R.</creatorcontrib><creatorcontrib>Li, Peigang</creatorcontrib><creatorcontrib>Greene, Andrew S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freed, Julie K.</au><au>Smith, Julia R.</au><au>Li, Peigang</au><au>Greene, Andrew S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of signal transduction complexes using biotin and crosslinking methodologies</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>7</volume><issue>14</issue><spage>2371</spage><epage>2374</epage><pages>2371-2374</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>We have developed a strategy to preferentially label the N‐terminal α‐amino groups of intact proteins allowing the internal ϵ‐amino groups to remain free to react with chemical crosslinking reagents. 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| ispartof | Proteomics (Weinheim), 2007-07, Vol.7 (14), p.2371-2374 |
| issn | 1615-9853 1615-9861 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Biotin Biotin - chemistry Cross-Linking Reagents - chemistry Crosslinking Fundamental and applied biological sciences. Psychology Miscellaneous Protein Binding Proteins Rats Receptors, Tumor Necrosis Factor - metabolism Signal Transduction Signaling Tandem Mass Spectrometry - methods TNF-alpha Tumor Necrosis Factor-alpha - metabolism |
| title | Isolation of signal transduction complexes using biotin and crosslinking methodologies |
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