REGULATION OF NOGO-B EXPRESSION IN THE LESION OF AORTIC ANEURYSMS
SUMMARY 1 Our previous study showed that Nogo‐B was highly expressed in endothelial cells and downregulated in endothelial cells following induction by lysophatidycholine, which contirubted to atherosclerotic lesions. However, the role of Nogo‐B in the development of aortic aneurysms remains unclear...
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| Veröffentlicht in: | Clinical and experimental pharmacology & physiology 2007-09, Vol.34 (9), p.856-860 |
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| container_title | Clinical and experimental pharmacology & physiology |
| container_volume | 34 |
| creator | Pan, Jing-Wei Wei, Meng Yang, Peng-Yuan Zheng, Xing Li, Jing-bo Lu, Zhi-gang Zhao, Xian-Xian Wu, Hong Kang, Hui Rui, Yao-cheng |
| description | SUMMARY
1
Our previous study showed that Nogo‐B was highly expressed in endothelial cells and downregulated in endothelial cells following induction by lysophatidycholine, which contirubted to atherosclerotic lesions. However, the role of Nogo‐B in the development of aortic aneurysms remains unclear.
2
In the present study, segments of thoracic aortic aneurysms (TAA) and adjacent normal thoracic aortic tissues (NTA) without aneurysmal changes were obtained from 31 patients undergoing graft surgery. The mRNA and protein expression levels of Nogo‐B were measured with semiquantitative reverse transcription–polymerase chain reaction, western blotting and immunohistochemistry.
3
The results demonstrate that Nogo‐B mRNA expression levels in TAA lesions decreased to 45% compared with levels in NTA lesions and that protein levels in TAA decreased to 35%. Tissue Nogo immunohistochemical staining in aortic specimens suggested the involvement of Nogo in neovascularization and smooth muscle cell proliferation. The weaker brown staining of endothelial cells in TAA lesions suggested the lower expression of Nogo‐B in TAA lesions.
4
These results demonstrate that Nogo‐B mRNA and protein expression are downregulated in TAA lesions. It is concluded that the reduction of Nogo‐B protein expression in TAA lesions is closely correlated to the formation of aneurysm and that Nogo‐B may play a protective role in the pathological process of aneurysms. |
| doi_str_mv | 10.1111/j.1440-1681.2007.04673.x |
| format | Article |
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1
Our previous study showed that Nogo‐B was highly expressed in endothelial cells and downregulated in endothelial cells following induction by lysophatidycholine, which contirubted to atherosclerotic lesions. However, the role of Nogo‐B in the development of aortic aneurysms remains unclear.
2
In the present study, segments of thoracic aortic aneurysms (TAA) and adjacent normal thoracic aortic tissues (NTA) without aneurysmal changes were obtained from 31 patients undergoing graft surgery. The mRNA and protein expression levels of Nogo‐B were measured with semiquantitative reverse transcription–polymerase chain reaction, western blotting and immunohistochemistry.
3
The results demonstrate that Nogo‐B mRNA expression levels in TAA lesions decreased to 45% compared with levels in NTA lesions and that protein levels in TAA decreased to 35%. Tissue Nogo immunohistochemical staining in aortic specimens suggested the involvement of Nogo in neovascularization and smooth muscle cell proliferation. The weaker brown staining of endothelial cells in TAA lesions suggested the lower expression of Nogo‐B in TAA lesions.
4
These results demonstrate that Nogo‐B mRNA and protein expression are downregulated in TAA lesions. It is concluded that the reduction of Nogo‐B protein expression in TAA lesions is closely correlated to the formation of aneurysm and that Nogo‐B may play a protective role in the pathological process of aneurysms.</description><identifier>ISSN: 0305-1870</identifier><identifier>EISSN: 1440-1681</identifier><identifier>DOI: 10.1111/j.1440-1681.2007.04673.x</identifier><identifier>PMID: 17645629</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>aneurysm ; Aorta, Thoracic - chemistry ; Aorta, Thoracic - pathology ; aortic aneurysm ; Aortic Aneurysm, Thoracic - genetics ; Aortic Aneurysm, Thoracic - metabolism ; Aortic Aneurysm, Thoracic - pathology ; atherosclerosis ; Blotting, Western ; dissecting/surgery ; Down-Regulation ; Humans ; Immunohistochemistry ; Myelin Proteins - analysis ; Myelin Proteins - genetics ; Nogo Proteins ; Nogo-B ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Tunica Intima - chemistry ; Tunica Intima - pathology</subject><ispartof>Clinical and experimental pharmacology & physiology, 2007-09, Vol.34 (9), p.856-860</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4053-b03de986decb37b6d21d7bf60317c70c917efc6df448bafc7134b8e71e0c52ce3</citedby><cites>FETCH-LOGICAL-c4053-b03de986decb37b6d21d7bf60317c70c917efc6df448bafc7134b8e71e0c52ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1681.2007.04673.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1681.2007.04673.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17645629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pan, Jing-Wei</creatorcontrib><creatorcontrib>Wei, Meng</creatorcontrib><creatorcontrib>Yang, Peng-Yuan</creatorcontrib><creatorcontrib>Zheng, Xing</creatorcontrib><creatorcontrib>Li, Jing-bo</creatorcontrib><creatorcontrib>Lu, Zhi-gang</creatorcontrib><creatorcontrib>Zhao, Xian-Xian</creatorcontrib><creatorcontrib>Wu, Hong</creatorcontrib><creatorcontrib>Kang, Hui</creatorcontrib><creatorcontrib>Rui, Yao-cheng</creatorcontrib><title>REGULATION OF NOGO-B EXPRESSION IN THE LESION OF AORTIC ANEURYSMS</title><title>Clinical and experimental pharmacology & physiology</title><addtitle>Clin Exp Pharmacol Physiol</addtitle><description>SUMMARY
1
Our previous study showed that Nogo‐B was highly expressed in endothelial cells and downregulated in endothelial cells following induction by lysophatidycholine, which contirubted to atherosclerotic lesions. However, the role of Nogo‐B in the development of aortic aneurysms remains unclear.
2
In the present study, segments of thoracic aortic aneurysms (TAA) and adjacent normal thoracic aortic tissues (NTA) without aneurysmal changes were obtained from 31 patients undergoing graft surgery. The mRNA and protein expression levels of Nogo‐B were measured with semiquantitative reverse transcription–polymerase chain reaction, western blotting and immunohistochemistry.
3
The results demonstrate that Nogo‐B mRNA expression levels in TAA lesions decreased to 45% compared with levels in NTA lesions and that protein levels in TAA decreased to 35%. Tissue Nogo immunohistochemical staining in aortic specimens suggested the involvement of Nogo in neovascularization and smooth muscle cell proliferation. The weaker brown staining of endothelial cells in TAA lesions suggested the lower expression of Nogo‐B in TAA lesions.
4
These results demonstrate that Nogo‐B mRNA and protein expression are downregulated in TAA lesions. It is concluded that the reduction of Nogo‐B protein expression in TAA lesions is closely correlated to the formation of aneurysm and that Nogo‐B may play a protective role in the pathological process of aneurysms.</description><subject>aneurysm</subject><subject>Aorta, Thoracic - chemistry</subject><subject>Aorta, Thoracic - pathology</subject><subject>aortic aneurysm</subject><subject>Aortic Aneurysm, Thoracic - genetics</subject><subject>Aortic Aneurysm, Thoracic - metabolism</subject><subject>Aortic Aneurysm, Thoracic - pathology</subject><subject>atherosclerosis</subject><subject>Blotting, Western</subject><subject>dissecting/surgery</subject><subject>Down-Regulation</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Myelin Proteins - analysis</subject><subject>Myelin Proteins - genetics</subject><subject>Nogo Proteins</subject><subject>Nogo-B</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Tunica Intima - chemistry</subject><subject>Tunica Intima - pathology</subject><issn>0305-1870</issn><issn>1440-1681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEFPwjAUxxujEUS_gtnJ2-br2rXj4GGSAUvGRrYR8dRsXZeAILhChG_vJgSvvktf-n7_95IfQgYGCzf1vLQwpWBi5mLLBuAWUMaJdbhC3cvgGnWBgGNil0MH3Wm9BAAHGLlFHcwZdZjd7yIv8Uez0MuCODLioRHFo9h8Nfz5NPHTtP0MIiMb-0bop2fEi5MsGBhe5M-S93SS3qObKl9p9XB-e2g29LPB2AzjUTDwQlNScIhZAClV32WlkgXhBSttXPKiYkAwlxxkH3NVSVZWlLpFXkmOCS1cxbEC6dhSkR56Ou3d1puvvdI7sV5oqVar_FNt9lpw4LRPwG5A9wTKeqN1rSqxrRfrvD4KDKLVJ5aitSRaS6LVJ371iUMTfTzf2BdrVf4Fz74a4OUEfC9W6vjvxWLgT9uuyZun_ELv1OGSz-sP0Yy5I96ikZiP2TQdTzKRkB832Ycd</recordid><startdate>200709</startdate><enddate>200709</enddate><creator>Pan, Jing-Wei</creator><creator>Wei, Meng</creator><creator>Yang, Peng-Yuan</creator><creator>Zheng, Xing</creator><creator>Li, Jing-bo</creator><creator>Lu, Zhi-gang</creator><creator>Zhao, Xian-Xian</creator><creator>Wu, Hong</creator><creator>Kang, Hui</creator><creator>Rui, Yao-cheng</creator><general>Blackwell Publishing Asia</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200709</creationdate><title>REGULATION OF NOGO-B EXPRESSION IN THE LESION OF AORTIC ANEURYSMS</title><author>Pan, Jing-Wei ; Wei, Meng ; Yang, Peng-Yuan ; Zheng, Xing ; Li, Jing-bo ; Lu, Zhi-gang ; Zhao, Xian-Xian ; Wu, Hong ; Kang, Hui ; Rui, Yao-cheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4053-b03de986decb37b6d21d7bf60317c70c917efc6df448bafc7134b8e71e0c52ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>aneurysm</topic><topic>Aorta, Thoracic - chemistry</topic><topic>Aorta, Thoracic - pathology</topic><topic>aortic aneurysm</topic><topic>Aortic Aneurysm, Thoracic - genetics</topic><topic>Aortic Aneurysm, Thoracic - metabolism</topic><topic>Aortic Aneurysm, Thoracic - pathology</topic><topic>atherosclerosis</topic><topic>Blotting, Western</topic><topic>dissecting/surgery</topic><topic>Down-Regulation</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Myelin Proteins - analysis</topic><topic>Myelin Proteins - genetics</topic><topic>Nogo Proteins</topic><topic>Nogo-B</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Tunica Intima - chemistry</topic><topic>Tunica Intima - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pan, Jing-Wei</creatorcontrib><creatorcontrib>Wei, Meng</creatorcontrib><creatorcontrib>Yang, Peng-Yuan</creatorcontrib><creatorcontrib>Zheng, Xing</creatorcontrib><creatorcontrib>Li, Jing-bo</creatorcontrib><creatorcontrib>Lu, Zhi-gang</creatorcontrib><creatorcontrib>Zhao, Xian-Xian</creatorcontrib><creatorcontrib>Wu, Hong</creatorcontrib><creatorcontrib>Kang, Hui</creatorcontrib><creatorcontrib>Rui, Yao-cheng</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental pharmacology & physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pan, Jing-Wei</au><au>Wei, Meng</au><au>Yang, Peng-Yuan</au><au>Zheng, Xing</au><au>Li, Jing-bo</au><au>Lu, Zhi-gang</au><au>Zhao, Xian-Xian</au><au>Wu, Hong</au><au>Kang, Hui</au><au>Rui, Yao-cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>REGULATION OF NOGO-B EXPRESSION IN THE LESION OF AORTIC ANEURYSMS</atitle><jtitle>Clinical and experimental pharmacology & physiology</jtitle><addtitle>Clin Exp Pharmacol Physiol</addtitle><date>2007-09</date><risdate>2007</risdate><volume>34</volume><issue>9</issue><spage>856</spage><epage>860</epage><pages>856-860</pages><issn>0305-1870</issn><eissn>1440-1681</eissn><abstract>SUMMARY
1
Our previous study showed that Nogo‐B was highly expressed in endothelial cells and downregulated in endothelial cells following induction by lysophatidycholine, which contirubted to atherosclerotic lesions. However, the role of Nogo‐B in the development of aortic aneurysms remains unclear.
2
In the present study, segments of thoracic aortic aneurysms (TAA) and adjacent normal thoracic aortic tissues (NTA) without aneurysmal changes were obtained from 31 patients undergoing graft surgery. The mRNA and protein expression levels of Nogo‐B were measured with semiquantitative reverse transcription–polymerase chain reaction, western blotting and immunohistochemistry.
3
The results demonstrate that Nogo‐B mRNA expression levels in TAA lesions decreased to 45% compared with levels in NTA lesions and that protein levels in TAA decreased to 35%. Tissue Nogo immunohistochemical staining in aortic specimens suggested the involvement of Nogo in neovascularization and smooth muscle cell proliferation. The weaker brown staining of endothelial cells in TAA lesions suggested the lower expression of Nogo‐B in TAA lesions.
4
These results demonstrate that Nogo‐B mRNA and protein expression are downregulated in TAA lesions. It is concluded that the reduction of Nogo‐B protein expression in TAA lesions is closely correlated to the formation of aneurysm and that Nogo‐B may play a protective role in the pathological process of aneurysms.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>17645629</pmid><doi>10.1111/j.1440-1681.2007.04673.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Clinical and experimental pharmacology & physiology, 2007-09, Vol.34 (9), p.856-860 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | aneurysm Aorta, Thoracic - chemistry Aorta, Thoracic - pathology aortic aneurysm Aortic Aneurysm, Thoracic - genetics Aortic Aneurysm, Thoracic - metabolism Aortic Aneurysm, Thoracic - pathology atherosclerosis Blotting, Western dissecting/surgery Down-Regulation Humans Immunohistochemistry Myelin Proteins - analysis Myelin Proteins - genetics Nogo Proteins Nogo-B Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Tunica Intima - chemistry Tunica Intima - pathology |
| title | REGULATION OF NOGO-B EXPRESSION IN THE LESION OF AORTIC ANEURYSMS |
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