A novel method to screen genomic libraries that combines genomic immunization with the prime-boost strategy
Abstract We employed a prime-boost regimen in combination with the expression library immunization protocol to improve the protective effectiveness of a genomic library used as immunogen. To demonstrate the feasibility of this novel strategy, we used as a prime a serogroup B Neisseria meningitidis r...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 2007-08, Vol.50 (3), p.430-433 |
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| container_title | FEMS immunology and medical microbiology |
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| creator | Yero, Daniel Pajón, Rolando Caballero, Evelin González, Sonia Cobas, Karem Fariñas, Mildrey Lopez, Yamilé Acosta, Armando |
| description | Abstract
We employed a prime-boost regimen in combination with the expression library immunization protocol to improve the protective effectiveness of a genomic library used as immunogen. To demonstrate the feasibility of this novel strategy, we used as a prime a serogroup B Neisseria meningitidis random genomic library constructed in a eukaryotic expression vector. Mice immunized with different fractions of this library and boosted with a single dose of meningococcal outer membrane vesicles elicited higher bactericidal antibody titers compared with mice primed with the empty vector. After the boost, passive administration of sera from mice primed with two of these fractions significantly reduced the number of viable bacteria in the blood of infant rats challenged with live N. meningitidis. The method proposed could be applied to the identification of subimmunogenic antigens during vaccine candidate screening by employing expression library immunization. |
| doi_str_mv | 10.1111/j.1574-695X.2007.00265.x |
| format | Article |
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We employed a prime-boost regimen in combination with the expression library immunization protocol to improve the protective effectiveness of a genomic library used as immunogen. To demonstrate the feasibility of this novel strategy, we used as a prime a serogroup B Neisseria meningitidis random genomic library constructed in a eukaryotic expression vector. Mice immunized with different fractions of this library and boosted with a single dose of meningococcal outer membrane vesicles elicited higher bactericidal antibody titers compared with mice primed with the empty vector. After the boost, passive administration of sera from mice primed with two of these fractions significantly reduced the number of viable bacteria in the blood of infant rats challenged with live N. meningitidis. The method proposed could be applied to the identification of subimmunogenic antigens during vaccine candidate screening by employing expression library immunization.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1111/j.1574-695X.2007.00265.x</identifier><identifier>PMID: 17537176</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies ; Antigens ; Antigens, Bacterial - genetics ; Antigens, Bacterial - immunology ; Antigens, Bacterial - isolation & purification ; Applied microbiology ; Bacterial Vaccines - genetics ; Bacterial Vaccines - immunology ; Bacterial Vaccines - isolation & purification ; Bacteriology ; Biological and medical sciences ; expression library immunization ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genomic Library ; Genomics ; Immunization ; Libraries ; Membrane vesicles ; Meningococcal Infections - blood ; Meningococcal Infections - prevention & control ; Mice ; Microbiology ; Miscellaneous ; Neisseria meningitidis ; Neisseria meningitidis - genetics ; Neisseria meningitidis - immunology ; outer membrane vesicles ; priming‐boost ; Rats ; Serum - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>FEMS immunology and medical microbiology, 2007-08, Vol.50 (3), p.430-433</ispartof><rights>2007 Federation of European Microbiological Societies 2007</rights><rights>2007 INIST-CNRS</rights><rights>2007 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4995-b19519b0188af961db7203b2883d6f7d744f14153c5e425631f1c4a03638ce473</citedby><cites>FETCH-LOGICAL-c4995-b19519b0188af961db7203b2883d6f7d744f14153c5e425631f1c4a03638ce473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-695X.2007.00265.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-695X.2007.00265.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18907040$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17537176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yero, Daniel</creatorcontrib><creatorcontrib>Pajón, Rolando</creatorcontrib><creatorcontrib>Caballero, Evelin</creatorcontrib><creatorcontrib>González, Sonia</creatorcontrib><creatorcontrib>Cobas, Karem</creatorcontrib><creatorcontrib>Fariñas, Mildrey</creatorcontrib><creatorcontrib>Lopez, Yamilé</creatorcontrib><creatorcontrib>Acosta, Armando</creatorcontrib><title>A novel method to screen genomic libraries that combines genomic immunization with the prime-boost strategy</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>Abstract
We employed a prime-boost regimen in combination with the expression library immunization protocol to improve the protective effectiveness of a genomic library used as immunogen. To demonstrate the feasibility of this novel strategy, we used as a prime a serogroup B Neisseria meningitidis random genomic library constructed in a eukaryotic expression vector. Mice immunized with different fractions of this library and boosted with a single dose of meningococcal outer membrane vesicles elicited higher bactericidal antibody titers compared with mice primed with the empty vector. After the boost, passive administration of sera from mice primed with two of these fractions significantly reduced the number of viable bacteria in the blood of infant rats challenged with live N. meningitidis. The method proposed could be applied to the identification of subimmunogenic antigens during vaccine candidate screening by employing expression library immunization.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - immunology</subject><subject>Antigens, Bacterial - isolation & purification</subject><subject>Applied microbiology</subject><subject>Bacterial Vaccines - genetics</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacterial Vaccines - isolation & purification</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>expression library immunization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genomic Library</subject><subject>Genomics</subject><subject>Immunization</subject><subject>Libraries</subject><subject>Membrane vesicles</subject><subject>Meningococcal Infections - blood</subject><subject>Meningococcal Infections - prevention & control</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Neisseria meningitidis</subject><subject>Neisseria meningitidis - genetics</subject><subject>Neisseria meningitidis - immunology</subject><subject>outer membrane vesicles</subject><subject>priming‐boost</subject><subject>Rats</subject><subject>Serum - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhiMEokvhLyBLCG4J44_4Q-JSVRQqFXEBiZvleJ2ulyRebId2-fU47EIlEAJfxtY878x43qpCGBpczsttg1vBaq7aTw0BEA0A4W1ze69a_Urcr1agiKwlYeykepTSFgCYAnhYnWDRUoEFX1Wfz9AUvroBjS5vwhrlgJKNzk3o2k1h9BYNvosmepdQ3piMbBg7P5XXz7wfx3ny30z2YUI3Pm8K59Au-tHVXQgpo5Sjye56_7h60JshuSfHeFp9vHj94fxtffX-zeX52VVtmVJt3WHVYtUBltL0iuN1JwjQjkhJ17wXa8FYjxluqW0dIy2nuMeWGaCcSuuYoKfVi0PdXQxfZpeyHn2ybhjM5MKctIBSQgH_J0iACcLZAj77DdyGOU7lE5pQ4ERyJpa-8kDZGFKKrtfLFkzcawx68U1v9WKPXuzRi2_6h2_6tkifHhvM3ejWd8KjUQV4fgRMsmboo5msT3ecVCCAQeFeHbgbP7j9fw-gLy7flUuR04M8zLu_iOs_p_8OBTXCJA</recordid><startdate>200708</startdate><enddate>200708</enddate><creator>Yero, Daniel</creator><creator>Pajón, Rolando</creator><creator>Caballero, Evelin</creator><creator>González, Sonia</creator><creator>Cobas, Karem</creator><creator>Fariñas, Mildrey</creator><creator>Lopez, Yamilé</creator><creator>Acosta, Armando</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7QL</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200708</creationdate><title>A novel method to screen genomic libraries that combines genomic immunization with the prime-boost strategy</title><author>Yero, Daniel ; Pajón, Rolando ; Caballero, Evelin ; González, Sonia ; Cobas, Karem ; Fariñas, Mildrey ; Lopez, Yamilé ; Acosta, Armando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4995-b19519b0188af961db7203b2883d6f7d744f14153c5e425631f1c4a03638ce473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Antigens, Bacterial - genetics</topic><topic>Antigens, Bacterial - immunology</topic><topic>Antigens, Bacterial - isolation & purification</topic><topic>Applied microbiology</topic><topic>Bacterial Vaccines - genetics</topic><topic>Bacterial Vaccines - immunology</topic><topic>Bacterial Vaccines - isolation & purification</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>expression library immunization</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Genomic Library</topic><topic>Genomics</topic><topic>Immunization</topic><topic>Libraries</topic><topic>Membrane vesicles</topic><topic>Meningococcal Infections - blood</topic><topic>Meningococcal Infections - prevention & control</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Neisseria meningitidis</topic><topic>Neisseria meningitidis - genetics</topic><topic>Neisseria meningitidis - immunology</topic><topic>outer membrane vesicles</topic><topic>priming‐boost</topic><topic>Rats</topic><topic>Serum - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yero, Daniel</creatorcontrib><creatorcontrib>Pajón, Rolando</creatorcontrib><creatorcontrib>Caballero, Evelin</creatorcontrib><creatorcontrib>González, Sonia</creatorcontrib><creatorcontrib>Cobas, Karem</creatorcontrib><creatorcontrib>Fariñas, Mildrey</creatorcontrib><creatorcontrib>Lopez, Yamilé</creatorcontrib><creatorcontrib>Acosta, Armando</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yero, Daniel</au><au>Pajón, Rolando</au><au>Caballero, Evelin</au><au>González, Sonia</au><au>Cobas, Karem</au><au>Fariñas, Mildrey</au><au>Lopez, Yamilé</au><au>Acosta, Armando</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel method to screen genomic libraries that combines genomic immunization with the prime-boost strategy</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2007-08</date><risdate>2007</risdate><volume>50</volume><issue>3</issue><spage>430</spage><epage>433</epage><pages>430-433</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Abstract
We employed a prime-boost regimen in combination with the expression library immunization protocol to improve the protective effectiveness of a genomic library used as immunogen. To demonstrate the feasibility of this novel strategy, we used as a prime a serogroup B Neisseria meningitidis random genomic library constructed in a eukaryotic expression vector. Mice immunized with different fractions of this library and boosted with a single dose of meningococcal outer membrane vesicles elicited higher bactericidal antibody titers compared with mice primed with the empty vector. After the boost, passive administration of sera from mice primed with two of these fractions significantly reduced the number of viable bacteria in the blood of infant rats challenged with live N. meningitidis. The method proposed could be applied to the identification of subimmunogenic antigens during vaccine candidate screening by employing expression library immunization.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17537176</pmid><doi>10.1111/j.1574-695X.2007.00265.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0928-8244 |
| ispartof | FEMS immunology and medical microbiology, 2007-08, Vol.50 (3), p.430-433 |
| issn | 0928-8244 1574-695X 2049-632X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70744906 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antibodies Antigens Antigens, Bacterial - genetics Antigens, Bacterial - immunology Antigens, Bacterial - isolation & purification Applied microbiology Bacterial Vaccines - genetics Bacterial Vaccines - immunology Bacterial Vaccines - isolation & purification Bacteriology Biological and medical sciences expression library immunization Fundamental and applied biological sciences. Psychology Gene expression Genomic Library Genomics Immunization Libraries Membrane vesicles Meningococcal Infections - blood Meningococcal Infections - prevention & control Mice Microbiology Miscellaneous Neisseria meningitidis Neisseria meningitidis - genetics Neisseria meningitidis - immunology outer membrane vesicles priming‐boost Rats Serum - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | A novel method to screen genomic libraries that combines genomic immunization with the prime-boost strategy |
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