Re-engineering a split-GFP reassembly screen to examine RING-domain interactions between BARD1 and BRCA1 mutants observed in cancer patients

Identification of protein-protein interactions is critical for understanding protein function and regulation. Split protein reassembly is an in vivo probe of protein interactions that circumvents some of the problems with yeast 2-hybrid (indirect interactions, false positives) and co-immunoprecipita...

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Veröffentlicht in:	Molecular bioSystems 2008, Vol.4 (6), p.599-605
Hauptverfasser:	Sarkar, Mohosin, Magliery, Thomas J
Format:	Artikel
Sprache:	eng
Schlagworte:	BRCA1 Protein - chemistry BRCA1 Protein - genetics BRCA1 Protein - metabolism Breast Neoplasms - genetics Green Fluorescent Proteins - analysis Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Humans Models, Molecular Mutant Proteins - chemistry Mutant Proteins - genetics Mutant Proteins - metabolism Protein Engineering Protein Folding Protein Structure, Tertiary Tumor Suppressor Proteins - chemistry Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - metabolism Ubiquitin-Protein Ligases - chemistry Ubiquitin-Protein Ligases - genetics Ubiquitin-Protein Ligases - metabolism
Online-Zugang:	Volltext
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