EglD, a putative endoglucanase, with an expansin like domain is localized in the conidial cell wall of Aspergillus nidulans
Although the process of conidial germination in filamentous fungi has been extensively studied, many aspects remain to be elucidated since the asexual spore or conidium is vital in their life cycle. Breakage and reformation of cell wall polymer bonds along with the maintenance of cell wall plasticit...
Gespeichert in:
| Veröffentlicht in: | Fungal genetics and biology 2008-06, Vol.45 (6), p.839-850 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 850 |
|---|---|
| container_issue | 6 |
| container_start_page | 839 |
| container_title | Fungal genetics and biology |
| container_volume | 45 |
| creator | Bouzarelou, Demetra Billini, Maria Roumelioti, Katerina Sophianopoulou, Vicky |
| description | Although the process of conidial germination in filamentous fungi has been extensively studied, many aspects remain to be elucidated since the asexual spore or conidium is vital in their life cycle. Breakage and reformation of cell wall polymer bonds along with the maintenance of cell wall plasticity during conidia germination depend upon a range of hydrolytic enzymes whose activity is analogous to that of expansins, a highly conserved group of plant cell wall proteins with characteristic wall loosening activity. In the current study, we identified and characterized the
eglD gene in
Aspergillus nidulans, an expansin-like gene the product of which shows strong similarities with bacterial and fungal endo-β1,4-glucanases. However, we failed to show such activity
in vitro. The
eglD gene is constitutively expressed in all developmental stages and compartments of
A. nidulans asexual life cycle. However, the EglD protein is exclusively present in conidial cell walls. The role of the EglD protein in morphogenesis, growth and germination rate of conidia was investigated. Our results show that EglD is a conidial cell wall localized expansin-like protein, which could be involved in cell wall remodeling during germination. |
| doi_str_mv | 10.1016/j.fgb.2008.03.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70740274</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1087184508000364</els_id><sourcerecordid>70740274</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-93c1c33cf4828d14eba240721b36d289f97074aa13cb52be0a7a7ffd83bd05a43</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EoqXwA7ggnzg1YfyxiSNOVSkfUqVeytma2JOtF28S4qSF8ufraFfiBhfPePy8r6x5GXsroBQgqg-7stu2pQQwJagSQDxjpwKaqoBG1c_X3tSFMHpzwl6ltMuA2Gjxkp3kGVSVMqfsz9U2fjrnyMdlxjncE6feD9u4OOwx0Tl_CPMdx57TrxH7FHoeww_ifthj7kPicXAYwyN5nu_zHXE39MEHjNxRjPwB8zF0_CKNNG1DjEvi-X2J2ew1e9FhTPTmWM_Y989Xt5dfi-ubL98uL64Lp4yci0Y54ZRynTbSeKGpRamhlqJVlZem6Zoaao0olGs3siXAGuuu80a1Hjao1Rl7f_Adp-HnQmm2-5DW32FPw5LsKgdZ_x-U0GhtKplBcQDdNKQ0UWfHKexx-m0F2DUau7M5GrtGY0HZvPmseXc0X9o9-b-KYxYZ-HgAKO_iPtBkkwvUO_JhIjdbP4R_2D8BivGfiQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20944862</pqid></control><display><type>article</type><title>EglD, a putative endoglucanase, with an expansin like domain is localized in the conidial cell wall of Aspergillus nidulans</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Bouzarelou, Demetra ; Billini, Maria ; Roumelioti, Katerina ; Sophianopoulou, Vicky</creator><creatorcontrib>Bouzarelou, Demetra ; Billini, Maria ; Roumelioti, Katerina ; Sophianopoulou, Vicky</creatorcontrib><description>Although the process of conidial germination in filamentous fungi has been extensively studied, many aspects remain to be elucidated since the asexual spore or conidium is vital in their life cycle. Breakage and reformation of cell wall polymer bonds along with the maintenance of cell wall plasticity during conidia germination depend upon a range of hydrolytic enzymes whose activity is analogous to that of expansins, a highly conserved group of plant cell wall proteins with characteristic wall loosening activity. In the current study, we identified and characterized the
eglD gene in
Aspergillus nidulans, an expansin-like gene the product of which shows strong similarities with bacterial and fungal endo-β1,4-glucanases. However, we failed to show such activity
in vitro. The
eglD gene is constitutively expressed in all developmental stages and compartments of
A. nidulans asexual life cycle. However, the EglD protein is exclusively present in conidial cell walls. The role of the EglD protein in morphogenesis, growth and germination rate of conidia was investigated. Our results show that EglD is a conidial cell wall localized expansin-like protein, which could be involved in cell wall remodeling during germination.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2008.03.001</identifier><identifier>PMID: 18406638</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Aspergillus nidulans ; Aspergillus nidulans - cytology ; Aspergillus nidulans - enzymology ; Aspergillus nidulans - growth & development ; Aspergillus nidulans - physiology ; Cell Cycle ; Cell Wall - enzymology ; Cell wall remodeling ; Cellulase - chemistry ; Cellulase - genetics ; Cellulase - metabolism ; Cloning, Molecular ; Conidial germination ; Endoglucanase ; Expansin-like protein ; Fungal Proteins - chemistry ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Expression Regulation, Fungal ; Gene Silencing ; Molecular Sequence Data ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Protein Structure, Tertiary ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; RNA Processing, Post-Transcriptional ; Sequence Alignment ; Spores, Fungal - enzymology ; Spores, Fungal - growth & development</subject><ispartof>Fungal genetics and biology, 2008-06, Vol.45 (6), p.839-850</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-93c1c33cf4828d14eba240721b36d289f97074aa13cb52be0a7a7ffd83bd05a43</citedby><cites>FETCH-LOGICAL-c382t-93c1c33cf4828d14eba240721b36d289f97074aa13cb52be0a7a7ffd83bd05a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fgb.2008.03.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18406638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bouzarelou, Demetra</creatorcontrib><creatorcontrib>Billini, Maria</creatorcontrib><creatorcontrib>Roumelioti, Katerina</creatorcontrib><creatorcontrib>Sophianopoulou, Vicky</creatorcontrib><title>EglD, a putative endoglucanase, with an expansin like domain is localized in the conidial cell wall of Aspergillus nidulans</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>Although the process of conidial germination in filamentous fungi has been extensively studied, many aspects remain to be elucidated since the asexual spore or conidium is vital in their life cycle. Breakage and reformation of cell wall polymer bonds along with the maintenance of cell wall plasticity during conidia germination depend upon a range of hydrolytic enzymes whose activity is analogous to that of expansins, a highly conserved group of plant cell wall proteins with characteristic wall loosening activity. In the current study, we identified and characterized the
eglD gene in
Aspergillus nidulans, an expansin-like gene the product of which shows strong similarities with bacterial and fungal endo-β1,4-glucanases. However, we failed to show such activity
in vitro. The
eglD gene is constitutively expressed in all developmental stages and compartments of
A. nidulans asexual life cycle. However, the EglD protein is exclusively present in conidial cell walls. The role of the EglD protein in morphogenesis, growth and germination rate of conidia was investigated. Our results show that EglD is a conidial cell wall localized expansin-like protein, which could be involved in cell wall remodeling during germination.</description><subject>Amino Acid Sequence</subject><subject>Aspergillus nidulans</subject><subject>Aspergillus nidulans - cytology</subject><subject>Aspergillus nidulans - enzymology</subject><subject>Aspergillus nidulans - growth & development</subject><subject>Aspergillus nidulans - physiology</subject><subject>Cell Cycle</subject><subject>Cell Wall - enzymology</subject><subject>Cell wall remodeling</subject><subject>Cellulase - chemistry</subject><subject>Cellulase - genetics</subject><subject>Cellulase - metabolism</subject><subject>Cloning, Molecular</subject><subject>Conidial germination</subject><subject>Endoglucanase</subject><subject>Expansin-like protein</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Gene Silencing</subject><subject>Molecular Sequence Data</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Protein Structure, Tertiary</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>Sequence Alignment</subject><subject>Spores, Fungal - enzymology</subject><subject>Spores, Fungal - growth & development</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EoqXwA7ggnzg1YfyxiSNOVSkfUqVeytma2JOtF28S4qSF8ufraFfiBhfPePy8r6x5GXsroBQgqg-7stu2pQQwJagSQDxjpwKaqoBG1c_X3tSFMHpzwl6ltMuA2Gjxkp3kGVSVMqfsz9U2fjrnyMdlxjncE6feD9u4OOwx0Tl_CPMdx57TrxH7FHoeww_ifthj7kPicXAYwyN5nu_zHXE39MEHjNxRjPwB8zF0_CKNNG1DjEvi-X2J2ew1e9FhTPTmWM_Y989Xt5dfi-ubL98uL64Lp4yci0Y54ZRynTbSeKGpRamhlqJVlZem6Zoaao0olGs3siXAGuuu80a1Hjao1Rl7f_Adp-HnQmm2-5DW32FPw5LsKgdZ_x-U0GhtKplBcQDdNKQ0UWfHKexx-m0F2DUau7M5GrtGY0HZvPmseXc0X9o9-b-KYxYZ-HgAKO_iPtBkkwvUO_JhIjdbP4R_2D8BivGfiQ</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Bouzarelou, Demetra</creator><creator>Billini, Maria</creator><creator>Roumelioti, Katerina</creator><creator>Sophianopoulou, Vicky</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080601</creationdate><title>EglD, a putative endoglucanase, with an expansin like domain is localized in the conidial cell wall of Aspergillus nidulans</title><author>Bouzarelou, Demetra ; Billini, Maria ; Roumelioti, Katerina ; Sophianopoulou, Vicky</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-93c1c33cf4828d14eba240721b36d289f97074aa13cb52be0a7a7ffd83bd05a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amino Acid Sequence</topic><topic>Aspergillus nidulans</topic><topic>Aspergillus nidulans - cytology</topic><topic>Aspergillus nidulans - enzymology</topic><topic>Aspergillus nidulans - growth & development</topic><topic>Aspergillus nidulans - physiology</topic><topic>Cell Cycle</topic><topic>Cell Wall - enzymology</topic><topic>Cell wall remodeling</topic><topic>Cellulase - chemistry</topic><topic>Cellulase - genetics</topic><topic>Cellulase - metabolism</topic><topic>Cloning, Molecular</topic><topic>Conidial germination</topic><topic>Endoglucanase</topic><topic>Expansin-like protein</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Gene Silencing</topic><topic>Molecular Sequence Data</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Protein Structure, Tertiary</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>Sequence Alignment</topic><topic>Spores, Fungal - enzymology</topic><topic>Spores, Fungal - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouzarelou, Demetra</creatorcontrib><creatorcontrib>Billini, Maria</creatorcontrib><creatorcontrib>Roumelioti, Katerina</creatorcontrib><creatorcontrib>Sophianopoulou, Vicky</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouzarelou, Demetra</au><au>Billini, Maria</au><au>Roumelioti, Katerina</au><au>Sophianopoulou, Vicky</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EglD, a putative endoglucanase, with an expansin like domain is localized in the conidial cell wall of Aspergillus nidulans</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>45</volume><issue>6</issue><spage>839</spage><epage>850</epage><pages>839-850</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>Although the process of conidial germination in filamentous fungi has been extensively studied, many aspects remain to be elucidated since the asexual spore or conidium is vital in their life cycle. Breakage and reformation of cell wall polymer bonds along with the maintenance of cell wall plasticity during conidia germination depend upon a range of hydrolytic enzymes whose activity is analogous to that of expansins, a highly conserved group of plant cell wall proteins with characteristic wall loosening activity. In the current study, we identified and characterized the
eglD gene in
Aspergillus nidulans, an expansin-like gene the product of which shows strong similarities with bacterial and fungal endo-β1,4-glucanases. However, we failed to show such activity
in vitro. The
eglD gene is constitutively expressed in all developmental stages and compartments of
A. nidulans asexual life cycle. However, the EglD protein is exclusively present in conidial cell walls. The role of the EglD protein in morphogenesis, growth and germination rate of conidia was investigated. Our results show that EglD is a conidial cell wall localized expansin-like protein, which could be involved in cell wall remodeling during germination.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18406638</pmid><doi>10.1016/j.fgb.2008.03.001</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-1845 |
| ispartof | Fungal genetics and biology, 2008-06, Vol.45 (6), p.839-850 |
| issn | 1087-1845 1096-0937 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70740274 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Amino Acid Sequence Aspergillus nidulans Aspergillus nidulans - cytology Aspergillus nidulans - enzymology Aspergillus nidulans - growth & development Aspergillus nidulans - physiology Cell Cycle Cell Wall - enzymology Cell wall remodeling Cellulase - chemistry Cellulase - genetics Cellulase - metabolism Cloning, Molecular Conidial germination Endoglucanase Expansin-like protein Fungal Proteins - chemistry Fungal Proteins - genetics Fungal Proteins - metabolism Gene Expression Regulation, Fungal Gene Silencing Molecular Sequence Data Plant Proteins - chemistry Plant Proteins - genetics Protein Structure, Tertiary Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism RNA Processing, Post-Transcriptional Sequence Alignment Spores, Fungal - enzymology Spores, Fungal - growth & development |
| title | EglD, a putative endoglucanase, with an expansin like domain is localized in the conidial cell wall of Aspergillus nidulans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T13%3A49%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=EglD,%20a%20putative%20endoglucanase,%20with%20an%20expansin%20like%20domain%20is%20localized%20in%20the%20conidial%20cell%20wall%20of%20Aspergillus%20nidulans&rft.jtitle=Fungal%20genetics%20and%20biology&rft.au=Bouzarelou,%20Demetra&rft.date=2008-06-01&rft.volume=45&rft.issue=6&rft.spage=839&rft.epage=850&rft.pages=839-850&rft.issn=1087-1845&rft.eissn=1096-0937&rft_id=info:doi/10.1016/j.fgb.2008.03.001&rft_dat=%3Cproquest_cross%3E70740274%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20944862&rft_id=info:pmid/18406638&rft_els_id=S1087184508000364&rfr_iscdi=true |