Chemical coding and central projections of gastric vagal afferent neurons
Vagal afferents that innervate gastric muscle or mucosa transmit distinct sensory information from their endings to the nucleus of the tractus solitarius (NTS). While these afferent subtypes are functionally distinct, no neurochemical correlate has been described and it is unknown whether they termi...
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| Veröffentlicht in: | Neurogastroenterology and motility 2008-06, Vol.20 (6), p.708-718 |
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| creator | Young, R. L. Cooper, N. J. Blackshaw, L. A. |
| description | Vagal afferents that innervate gastric muscle or mucosa transmit distinct sensory information from their endings to the nucleus of the tractus solitarius (NTS). While these afferent subtypes are functionally distinct, no neurochemical correlate has been described and it is unknown whether they terminate in different central locations. This study aimed to identify gastric vagal afferent subtypes in the nodose ganglion (NG) of ferrets, their terminal areas in NTS and neurochemistry for isolectin‐B4 (IB4) and calcitonin gene‐related peptide (CGRP). Vagal afferents were traced from gastric muscle or mucosa and IB4 and CGRP labelling assessed in NG and NTS. 7 ± 1% and 6 ± 1% of NG neurons were traced from gastric muscle or mucosa respectively; these were more likely to label for CGRP or for both CGRP and IB4 than other NG neurons (P |
| doi_str_mv | 10.1111/j.1365-2982.2007.01071.x |
| format | Article |
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L. ; Cooper, N. J. ; Blackshaw, L. A.</creator><creatorcontrib>Young, R. L. ; Cooper, N. J. ; Blackshaw, L. A.</creatorcontrib><description>Vagal afferents that innervate gastric muscle or mucosa transmit distinct sensory information from their endings to the nucleus of the tractus solitarius (NTS). While these afferent subtypes are functionally distinct, no neurochemical correlate has been described and it is unknown whether they terminate in different central locations. This study aimed to identify gastric vagal afferent subtypes in the nodose ganglion (NG) of ferrets, their terminal areas in NTS and neurochemistry for isolectin‐B4 (IB4) and calcitonin gene‐related peptide (CGRP). Vagal afferents were traced from gastric muscle or mucosa and IB4 and CGRP labelling assessed in NG and NTS. 7 ± 1% and 6 ± 1% of NG neurons were traced from gastric muscle or mucosa respectively; these were more likely to label for CGRP or for both CGRP and IB4 than other NG neurons (P < 0.01). Muscular afferents were also less likely than others to label with IB4 (P < 0.001). Less than 1% of NG neurons were traced from both muscle and mucosa. Central terminals of both afferent subtypes occurred in the subnucleus gelatinosus of the NTS, but did not overlap completely. This region also labelled for CGRP and IB4. We conclude that while vagal afferents from gastric muscle and mucosa differ little in their chemical coding for CGRP and IB4, they can be traced selectively from their peripheral endings to NG and to overlapping and distinct regions of NTS. Thus, there is an anatomical substrate for convergent NTS integration for both types of afferent input.</description><identifier>ISSN: 1350-1925</identifier><identifier>EISSN: 1365-2982</identifier><identifier>DOI: 10.1111/j.1365-2982.2007.01071.x</identifier><identifier>PMID: 18266614</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Calcitonin Gene-Related Peptide - analysis ; calcitonin gene‐related peptide ; Ferrets ; immunohistochemistry ; isolectin‐B4 ; Male ; Mustela ; nerve tracing ; Neural Pathways - chemistry ; Neural Pathways - physiology ; Neurons, Afferent - chemistry ; Neurons, Afferent - physiology ; Nodose Ganglion - chemistry ; Nodose Ganglion - physiology ; nucleus tractus solitarii ; Staining and Labeling - methods ; Stomach - chemistry ; Stomach - innervation ; Stomach - physiology ; vagal afferent ; Vagus Nerve - chemistry ; Vagus Nerve - physiology</subject><ispartof>Neurogastroenterology and motility, 2008-06, Vol.20 (6), p.708-718</ispartof><rights>2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4641-cf2a8d43fd60175e8f1077bcda8572f4fbc3513c1d0d2a220173e42490fcd42e3</citedby><cites>FETCH-LOGICAL-c4641-cf2a8d43fd60175e8f1077bcda8572f4fbc3513c1d0d2a220173e42490fcd42e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2982.2007.01071.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2982.2007.01071.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18266614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Young, R. L.</creatorcontrib><creatorcontrib>Cooper, N. J.</creatorcontrib><creatorcontrib>Blackshaw, L. A.</creatorcontrib><title>Chemical coding and central projections of gastric vagal afferent neurons</title><title>Neurogastroenterology and motility</title><addtitle>Neurogastroenterol Motil</addtitle><description>Vagal afferents that innervate gastric muscle or mucosa transmit distinct sensory information from their endings to the nucleus of the tractus solitarius (NTS). While these afferent subtypes are functionally distinct, no neurochemical correlate has been described and it is unknown whether they terminate in different central locations. This study aimed to identify gastric vagal afferent subtypes in the nodose ganglion (NG) of ferrets, their terminal areas in NTS and neurochemistry for isolectin‐B4 (IB4) and calcitonin gene‐related peptide (CGRP). Vagal afferents were traced from gastric muscle or mucosa and IB4 and CGRP labelling assessed in NG and NTS. 7 ± 1% and 6 ± 1% of NG neurons were traced from gastric muscle or mucosa respectively; these were more likely to label for CGRP or for both CGRP and IB4 than other NG neurons (P < 0.01). Muscular afferents were also less likely than others to label with IB4 (P < 0.001). Less than 1% of NG neurons were traced from both muscle and mucosa. Central terminals of both afferent subtypes occurred in the subnucleus gelatinosus of the NTS, but did not overlap completely. This region also labelled for CGRP and IB4. We conclude that while vagal afferents from gastric muscle and mucosa differ little in their chemical coding for CGRP and IB4, they can be traced selectively from their peripheral endings to NG and to overlapping and distinct regions of NTS. Thus, there is an anatomical substrate for convergent NTS integration for both types of afferent input.</description><subject>Animals</subject><subject>Calcitonin Gene-Related Peptide - analysis</subject><subject>calcitonin gene‐related peptide</subject><subject>Ferrets</subject><subject>immunohistochemistry</subject><subject>isolectin‐B4</subject><subject>Male</subject><subject>Mustela</subject><subject>nerve tracing</subject><subject>Neural Pathways - chemistry</subject><subject>Neural Pathways - physiology</subject><subject>Neurons, Afferent - chemistry</subject><subject>Neurons, Afferent - physiology</subject><subject>Nodose Ganglion - chemistry</subject><subject>Nodose Ganglion - physiology</subject><subject>nucleus tractus solitarii</subject><subject>Staining and Labeling - methods</subject><subject>Stomach - chemistry</subject><subject>Stomach - innervation</subject><subject>Stomach - physiology</subject><subject>vagal afferent</subject><subject>Vagus Nerve - chemistry</subject><subject>Vagus Nerve - physiology</subject><issn>1350-1925</issn><issn>1365-2982</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMlOwzAQQC0EoqXwCygnbgneYjsHDqhiqVToBc6W66UkylLsFNq_x6EVHGEuM_K8GY8eAAmCGYpxXWWIsDzFhcAZhpBnEEGOsu0RGP80joc6hykqcD4CZyFUEEKGKTsFIyQwYwzRMZhN32xTalUnujNlu0pUaxJt297Hp7XvKqv7smtD0rlkpULvS518qFVsKuesj2DS2o2PxDk4caoO9uKQJ-D1_u5l-pjOFw-z6e081ZRRlGqHlTCUOMMg4rkVLp7Ol9ookXPsqFtqkiOikYEGK4wjRCzFtIBOG4otmYCr_d543fvGhl42ZdC2rlVru02QHHJS5Jz-CWJIWSFEEUGxB7XvQvDWybUvG-V3EkE5-JaVHLTKQascfMtv33IbRy8Pf2yWjTW_gwfBEbjZA59lbXf_XiyfnxZDRb4AF0mORA</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Young, R. L.</creator><creator>Cooper, N. J.</creator><creator>Blackshaw, L. A.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>Chemical coding and central projections of gastric vagal afferent neurons</title><author>Young, R. L. ; Cooper, N. J. ; Blackshaw, L. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4641-cf2a8d43fd60175e8f1077bcda8572f4fbc3513c1d0d2a220173e42490fcd42e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Calcitonin Gene-Related Peptide - analysis</topic><topic>calcitonin gene‐related peptide</topic><topic>Ferrets</topic><topic>immunohistochemistry</topic><topic>isolectin‐B4</topic><topic>Male</topic><topic>Mustela</topic><topic>nerve tracing</topic><topic>Neural Pathways - chemistry</topic><topic>Neural Pathways - physiology</topic><topic>Neurons, Afferent - chemistry</topic><topic>Neurons, Afferent - physiology</topic><topic>Nodose Ganglion - chemistry</topic><topic>Nodose Ganglion - physiology</topic><topic>nucleus tractus solitarii</topic><topic>Staining and Labeling - methods</topic><topic>Stomach - chemistry</topic><topic>Stomach - innervation</topic><topic>Stomach - physiology</topic><topic>vagal afferent</topic><topic>Vagus Nerve - chemistry</topic><topic>Vagus Nerve - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Young, R. L.</creatorcontrib><creatorcontrib>Cooper, N. J.</creatorcontrib><creatorcontrib>Blackshaw, L. A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neurogastroenterology and motility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Young, R. L.</au><au>Cooper, N. J.</au><au>Blackshaw, L. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical coding and central projections of gastric vagal afferent neurons</atitle><jtitle>Neurogastroenterology and motility</jtitle><addtitle>Neurogastroenterol Motil</addtitle><date>2008-06</date><risdate>2008</risdate><volume>20</volume><issue>6</issue><spage>708</spage><epage>718</epage><pages>708-718</pages><issn>1350-1925</issn><eissn>1365-2982</eissn><abstract>Vagal afferents that innervate gastric muscle or mucosa transmit distinct sensory information from their endings to the nucleus of the tractus solitarius (NTS). While these afferent subtypes are functionally distinct, no neurochemical correlate has been described and it is unknown whether they terminate in different central locations. This study aimed to identify gastric vagal afferent subtypes in the nodose ganglion (NG) of ferrets, their terminal areas in NTS and neurochemistry for isolectin‐B4 (IB4) and calcitonin gene‐related peptide (CGRP). Vagal afferents were traced from gastric muscle or mucosa and IB4 and CGRP labelling assessed in NG and NTS. 7 ± 1% and 6 ± 1% of NG neurons were traced from gastric muscle or mucosa respectively; these were more likely to label for CGRP or for both CGRP and IB4 than other NG neurons (P < 0.01). Muscular afferents were also less likely than others to label with IB4 (P < 0.001). Less than 1% of NG neurons were traced from both muscle and mucosa. Central terminals of both afferent subtypes occurred in the subnucleus gelatinosus of the NTS, but did not overlap completely. This region also labelled for CGRP and IB4. We conclude that while vagal afferents from gastric muscle and mucosa differ little in their chemical coding for CGRP and IB4, they can be traced selectively from their peripheral endings to NG and to overlapping and distinct regions of NTS. Thus, there is an anatomical substrate for convergent NTS integration for both types of afferent input.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18266614</pmid><doi>10.1111/j.1365-2982.2007.01071.x</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Calcitonin Gene-Related Peptide - analysis calcitonin gene‐related peptide Ferrets immunohistochemistry isolectin‐B4 Male Mustela nerve tracing Neural Pathways - chemistry Neural Pathways - physiology Neurons, Afferent - chemistry Neurons, Afferent - physiology Nodose Ganglion - chemistry Nodose Ganglion - physiology nucleus tractus solitarii Staining and Labeling - methods Stomach - chemistry Stomach - innervation Stomach - physiology vagal afferent Vagus Nerve - chemistry Vagus Nerve - physiology |
| title | Chemical coding and central projections of gastric vagal afferent neurons |
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