Regulators of G-protein signaling 4 in adrenal gland: localization, regulation, and role in aldosterone secretion
Regulators of G-protein signaling (RGS proteins) interact with Gα subunits of heterotrimeric G-proteins, accelerating the rate of GTP hydrolysis and finalizing the intracellular signaling triggered by the G-protein-coupled receptor (GPCR)–ligand interaction. Angiotensin II (Ang II) interacts with it...
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Veröffentlicht in: | Journal of endocrinology 2007-08, Vol.194 (2), p.429-440 |
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description | Regulators of G-protein signaling (RGS proteins) interact with Gα subunits of heterotrimeric G-proteins, accelerating the rate of GTP hydrolysis and finalizing the intracellular signaling triggered by the G-protein-coupled receptor (GPCR)–ligand interaction. Angiotensin II (Ang II) interacts with its GPCR in adrenal zona glomerulosa cells and triggers a cascade of intracellular signals that regulates steroidogenesis and proliferation. On screening for adrenal zona glomerulosa-specific genes, we found that RGS4 was exclusively localized in the zona glomerulosa of the rat adrenal cortex. We studied RGS4 expression and regulation in the rat adrenal gland, including the signaling pathways involved, as well as the role of RGS4 in steroidogenesis in human adrenocortical H295R cells. We reported that RGS4 mRNA expression in the rat adrenal gland was restricted to the adrenal zonal glomerulosa and upregulated by low-salt diet and Ang II infusion in rat adrenal glands in vivo. In H295R cells, Ang II caused a rapid and transient increase in RGS4 mRNA levels mediated by the calcium/calmodulin/calmodulin-dependent protein kinase and protein kinase C pathways. RGS4 overexpression by retroviral infection in H295R cells decreased Ang II-stimulated aldosterone secretion. In reporter assays, RGS4 decreased Ang II-mediated aldosterone synthase upregulation. In , RGS4 is an adrenal gland zona glomerulosa-specific gene that is upregulated by aldosterone secretagogues, in vivo and in vitro, and functions as a negative feedback of Ang II-triggered intracellular signaling. Alterations in RGS4 expression levels or functions may be involved in deregulations of Ang II signaling and abnormal aldosterone secretion. |
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Angiotensin II (Ang II) interacts with its GPCR in adrenal zona glomerulosa cells and triggers a cascade of intracellular signals that regulates steroidogenesis and proliferation. On screening for adrenal zona glomerulosa-specific genes, we found that RGS4 was exclusively localized in the zona glomerulosa of the rat adrenal cortex. We studied RGS4 expression and regulation in the rat adrenal gland, including the signaling pathways involved, as well as the role of RGS4 in steroidogenesis in human adrenocortical H295R cells. We reported that RGS4 mRNA expression in the rat adrenal gland was restricted to the adrenal zonal glomerulosa and upregulated by low-salt diet and Ang II infusion in rat adrenal glands in vivo. In H295R cells, Ang II caused a rapid and transient increase in RGS4 mRNA levels mediated by the calcium/calmodulin/calmodulin-dependent protein kinase and protein kinase C pathways. RGS4 overexpression by retroviral infection in H295R cells decreased Ang II-stimulated aldosterone secretion. In reporter assays, RGS4 decreased Ang II-mediated aldosterone synthase upregulation. In , RGS4 is an adrenal gland zona glomerulosa-specific gene that is upregulated by aldosterone secretagogues, in vivo and in vitro, and functions as a negative feedback of Ang II-triggered intracellular signaling. Alterations in RGS4 expression levels or functions may be involved in deregulations of Ang II signaling and abnormal aldosterone secretion.</description><identifier>ISSN: 0022-0795</identifier><identifier>EISSN: 1479-6805</identifier><identifier>DOI: 10.1677/JOE-07-0153</identifier><identifier>PMID: 17641290</identifier><language>eng</language><publisher>England: BioScientifica</publisher><subject>Adolescent ; Adult ; Aldosterone - metabolism ; Aldosterone - secretion ; Angiotensin II - metabolism ; Angiotensin II - pharmacology ; Animals ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 ; Calcium-Calmodulin-Dependent Protein Kinases - metabolism ; Cell Line ; Diet, Sodium-Restricted ; Female ; Genetic Vectors - administration & dosage ; GTP-Binding Proteins - metabolism ; Humans ; Male ; Middle Aged ; Protein Kinase C - metabolism ; Rats ; Rats, Sprague-Dawley ; Regular papers ; Retroviridae - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RGS Proteins - genetics ; RNA, Messenger - analysis ; Signal Transduction - physiology ; Transduction, Genetic ; Up-Regulation ; Zona Glomerulosa - chemistry ; Zona Glomerulosa - metabolism ; Zona Glomerulosa - secretion</subject><ispartof>Journal of endocrinology, 2007-08, Vol.194 (2), p.429-440</ispartof><rights>2007 Society for Endocrinology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b506t-7ff543338beded23cb9c5fce13b38901a146849e2b5cdc93e3ddea04d97435783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17641290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Romero, Damian G</creatorcontrib><creatorcontrib>Zhou, Ming Yi</creatorcontrib><creatorcontrib>Yanes, Licy L</creatorcontrib><creatorcontrib>Plonczynski, Maria W</creatorcontrib><creatorcontrib>Washington, Tanganika R</creatorcontrib><creatorcontrib>Gomez-Sanchez, Celso E</creatorcontrib><creatorcontrib>Gomez-Sanchez, Elise P</creatorcontrib><title>Regulators of G-protein signaling 4 in adrenal gland: localization, regulation, and role in aldosterone secretion</title><title>Journal of endocrinology</title><addtitle>J Endocrinol</addtitle><description>Regulators of G-protein signaling (RGS proteins) interact with Gα subunits of heterotrimeric G-proteins, accelerating the rate of GTP hydrolysis and finalizing the intracellular signaling triggered by the G-protein-coupled receptor (GPCR)–ligand interaction. Angiotensin II (Ang II) interacts with its GPCR in adrenal zona glomerulosa cells and triggers a cascade of intracellular signals that regulates steroidogenesis and proliferation. On screening for adrenal zona glomerulosa-specific genes, we found that RGS4 was exclusively localized in the zona glomerulosa of the rat adrenal cortex. We studied RGS4 expression and regulation in the rat adrenal gland, including the signaling pathways involved, as well as the role of RGS4 in steroidogenesis in human adrenocortical H295R cells. We reported that RGS4 mRNA expression in the rat adrenal gland was restricted to the adrenal zonal glomerulosa and upregulated by low-salt diet and Ang II infusion in rat adrenal glands in vivo. In H295R cells, Ang II caused a rapid and transient increase in RGS4 mRNA levels mediated by the calcium/calmodulin/calmodulin-dependent protein kinase and protein kinase C pathways. RGS4 overexpression by retroviral infection in H295R cells decreased Ang II-stimulated aldosterone secretion. In reporter assays, RGS4 decreased Ang II-mediated aldosterone synthase upregulation. In , RGS4 is an adrenal gland zona glomerulosa-specific gene that is upregulated by aldosterone secretagogues, in vivo and in vitro, and functions as a negative feedback of Ang II-triggered intracellular signaling. Alterations in RGS4 expression levels or functions may be involved in deregulations of Ang II signaling and abnormal aldosterone secretion.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aldosterone - metabolism</subject><subject>Aldosterone - secretion</subject><subject>Angiotensin II - metabolism</subject><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</subject><subject>Cell Line</subject><subject>Diet, Sodium-Restricted</subject><subject>Female</subject><subject>Genetic Vectors - administration & dosage</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Protein Kinase C - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regular papers</subject><subject>Retroviridae - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RGS Proteins - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>Signal Transduction - physiology</subject><subject>Transduction, Genetic</subject><subject>Up-Regulation</subject><subject>Zona Glomerulosa - chemistry</subject><subject>Zona Glomerulosa - metabolism</subject><subject>Zona Glomerulosa - secretion</subject><issn>0022-0795</issn><issn>1479-6805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPxCAUhYnR6PhYuTes3GgVCi3FnZn4jImJ0TWhcFsxnaLQidFfL2PHaFzoCm7Od064HIR2KTmipRDH17dnGREZoQVbQRPKhczKihSraEJInidJFhtoM8YnkhAq2DraoKLkNJdkgl7uoJ13evAhYt_gi-w5-AFcj6Nre925vsUcp1HbAGnGbad7e4I7b5L4rgfn-0McxozPe5Jx8B18mjrr4wDB94AjmAALZButNbqLsLM8t9DD-dn99DK7ub24mp7eZHVByiETTVNwxlhVgwWbM1NLUzQGKKtZJQnVlJcVl5DXhbFGMmDWgibcSsFZISq2hfbH3LTRyxzioGYuGujSAuDnUQkimKjK8l-QSilJVfIEHoygCT7GAI16Dm6mw5uiRC2qUKkKRYRaVJHovWXsvJ6B_WaXf5-AfAQeXfv46gKo2vloHPSDa5zRP1O_Ck4mOpp-sX-95AMYQaWG</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Romero, Damian G</creator><creator>Zhou, Ming Yi</creator><creator>Yanes, Licy L</creator><creator>Plonczynski, Maria W</creator><creator>Washington, Tanganika R</creator><creator>Gomez-Sanchez, Celso E</creator><creator>Gomez-Sanchez, Elise P</creator><general>BioScientifica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20070801</creationdate><title>Regulators of G-protein signaling 4 in adrenal gland: localization, regulation, and role in aldosterone secretion</title><author>Romero, Damian G ; Zhou, Ming Yi ; Yanes, Licy L ; Plonczynski, Maria W ; Washington, Tanganika R ; Gomez-Sanchez, Celso E ; Gomez-Sanchez, Elise P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b506t-7ff543338beded23cb9c5fce13b38901a146849e2b5cdc93e3ddea04d97435783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aldosterone - metabolism</topic><topic>Aldosterone - secretion</topic><topic>Angiotensin II - metabolism</topic><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Calcium-Calmodulin-Dependent Protein Kinase Type 2</topic><topic>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</topic><topic>Cell Line</topic><topic>Diet, Sodium-Restricted</topic><topic>Female</topic><topic>Genetic Vectors - administration & dosage</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Protein Kinase C - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regular papers</topic><topic>Retroviridae - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RGS Proteins - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Signal Transduction - physiology</topic><topic>Transduction, Genetic</topic><topic>Up-Regulation</topic><topic>Zona Glomerulosa - chemistry</topic><topic>Zona Glomerulosa - metabolism</topic><topic>Zona Glomerulosa - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Romero, Damian G</creatorcontrib><creatorcontrib>Zhou, Ming Yi</creatorcontrib><creatorcontrib>Yanes, Licy L</creatorcontrib><creatorcontrib>Plonczynski, Maria W</creatorcontrib><creatorcontrib>Washington, Tanganika R</creatorcontrib><creatorcontrib>Gomez-Sanchez, Celso E</creatorcontrib><creatorcontrib>Gomez-Sanchez, Elise P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Romero, Damian G</au><au>Zhou, Ming Yi</au><au>Yanes, Licy L</au><au>Plonczynski, Maria W</au><au>Washington, Tanganika R</au><au>Gomez-Sanchez, Celso E</au><au>Gomez-Sanchez, Elise P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulators of G-protein signaling 4 in adrenal gland: localization, regulation, and role in aldosterone secretion</atitle><jtitle>Journal of endocrinology</jtitle><addtitle>J Endocrinol</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>194</volume><issue>2</issue><spage>429</spage><epage>440</epage><pages>429-440</pages><issn>0022-0795</issn><eissn>1479-6805</eissn><abstract>Regulators of G-protein signaling (RGS proteins) interact with Gα subunits of heterotrimeric G-proteins, accelerating the rate of GTP hydrolysis and finalizing the intracellular signaling triggered by the G-protein-coupled receptor (GPCR)–ligand interaction. Angiotensin II (Ang II) interacts with its GPCR in adrenal zona glomerulosa cells and triggers a cascade of intracellular signals that regulates steroidogenesis and proliferation. On screening for adrenal zona glomerulosa-specific genes, we found that RGS4 was exclusively localized in the zona glomerulosa of the rat adrenal cortex. We studied RGS4 expression and regulation in the rat adrenal gland, including the signaling pathways involved, as well as the role of RGS4 in steroidogenesis in human adrenocortical H295R cells. We reported that RGS4 mRNA expression in the rat adrenal gland was restricted to the adrenal zonal glomerulosa and upregulated by low-salt diet and Ang II infusion in rat adrenal glands in vivo. In H295R cells, Ang II caused a rapid and transient increase in RGS4 mRNA levels mediated by the calcium/calmodulin/calmodulin-dependent protein kinase and protein kinase C pathways. RGS4 overexpression by retroviral infection in H295R cells decreased Ang II-stimulated aldosterone secretion. In reporter assays, RGS4 decreased Ang II-mediated aldosterone synthase upregulation. In , RGS4 is an adrenal gland zona glomerulosa-specific gene that is upregulated by aldosterone secretagogues, in vivo and in vitro, and functions as a negative feedback of Ang II-triggered intracellular signaling. Alterations in RGS4 expression levels or functions may be involved in deregulations of Ang II signaling and abnormal aldosterone secretion.</abstract><cop>England</cop><pub>BioScientifica</pub><pmid>17641290</pmid><doi>10.1677/JOE-07-0153</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adolescent Adult Aldosterone - metabolism Aldosterone - secretion Angiotensin II - metabolism Angiotensin II - pharmacology Animals Calcium-Calmodulin-Dependent Protein Kinase Type 2 Calcium-Calmodulin-Dependent Protein Kinases - metabolism Cell Line Diet, Sodium-Restricted Female Genetic Vectors - administration & dosage GTP-Binding Proteins - metabolism Humans Male Middle Aged Protein Kinase C - metabolism Rats Rats, Sprague-Dawley Regular papers Retroviridae - genetics Reverse Transcriptase Polymerase Chain Reaction RGS Proteins - genetics RNA, Messenger - analysis Signal Transduction - physiology Transduction, Genetic Up-Regulation Zona Glomerulosa - chemistry Zona Glomerulosa - metabolism Zona Glomerulosa - secretion |
title | Regulators of G-protein signaling 4 in adrenal gland: localization, regulation, and role in aldosterone secretion |
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