Expression of the retinoic acid-metabolizing enzymes RALDH2 and CYP26b1 during mouse postnatal testis development
Aim： To study the expression pattern of the retinoic acid metabolizing enzymes RALDH2 and CYP26bl during mouse postnatal testis development at both mRNA and protein levels. Methods： Real-time polymerase chain reaction and Western blot analysis were performed to determine the relative quantity of RAL...
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| description | Aim： To study the expression pattern of the retinoic acid metabolizing enzymes RALDH2 and CYP26bl during mouse postnatal testis development at both mRNA and protein levels. Methods： Real-time polymerase chain reaction and Western blot analysis were performed to determine the relative quantity of RALDH2 and CYP26bl at both mRNA and protein levels at postnatal day 1, 5, 10, 20, and in adult mice （70 days testes）. Testicular localization of RALDH2 and CYP26b 1 during mouse postnatal development was examined using immunohistochemistry assay. Results： Aldhla2 transcripts and its protein RALDH2 began to increase at postnatal day 10, and remained at a high level through postnatal day 20 to adulthood. Cyp2661 transcripts and CYP26bl protein did not change significantly during mouse postnatal testis development. RALDH2 was undetectable in the postnatal 1, 5 and 10 day testes using immunohis- tochemistry assay. At postnatal day 20 it was detected in pachytene spermatocytes. Robust expression of RALDH2 was restricted in round spermatids in the adult mouse testis. In the developing and adult testis, CYP26bl protein was confined to the peritubular myoepithelial cells. Conclusion： Our results indicate that following birth, the level of retinoic acid in the seminiferous tubules might begin to increase at postnatal day 10, and maintain a high level through postnatal day 20 to adulthood. |
| doi_str_mv | 10.1111/j.1745-7262.2008.00408.x |
| format | Article |
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Methods： Real-time polymerase chain reaction and Western blot analysis were performed to determine the relative quantity of RALDH2 and CYP26bl at both mRNA and protein levels at postnatal day 1, 5, 10, 20, and in adult mice （70 days testes）. Testicular localization of RALDH2 and CYP26b 1 during mouse postnatal development was examined using immunohistochemistry assay. Results： Aldhla2 transcripts and its protein RALDH2 began to increase at postnatal day 10, and remained at a high level through postnatal day 20 to adulthood. Cyp2661 transcripts and CYP26bl protein did not change significantly during mouse postnatal testis development. RALDH2 was undetectable in the postnatal 1, 5 and 10 day testes using immunohis- tochemistry assay. At postnatal day 20 it was detected in pachytene spermatocytes. Robust expression of RALDH2 was restricted in round spermatids in the adult mouse testis. In the developing and adult testis, CYP26bl protein was confined to the peritubular myoepithelial cells. Conclusion： Our results indicate that following birth, the level of retinoic acid in the seminiferous tubules might begin to increase at postnatal day 10, and maintain a high level through postnatal day 20 to adulthood.</description><identifier>ISSN: 1008-682X</identifier><identifier>EISSN: 1745-7262</identifier><identifier>DOI: 10.1111/j.1745-7262.2008.00408.x</identifier><identifier>PMID: 18478160</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aldehyde Oxidoreductases - genetics ; Aldehyde Oxidoreductases - metabolism ; Animals ; CYP26b1 ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Gene Expression Regulation, Developmental ; Male ; Mice ; Mice, Inbred BALB C ; Rabbits ; RALDH2 ; retinoic acid ; Retinoic Acid 4-Hydroxylase ; RNA, Messenger - metabolism ; Seminiferous Epithelium - cytology ; Seminiferous Epithelium - metabolism ; Sensitivity and Specificity ; Spermatids - cytology ; Spermatids - metabolism ; spermatogenesis ; testis ; Testis - cytology ; Testis - growth & development ; Testis - metabolism ; 出生后睾丸发育 ; 类维生素A酸 ; 精子发生</subject><ispartof>Asian journal of andrology, 2008-07, Vol.10 (4), p.569-576</ispartof><rights>2008, Asian Journal of Andrology, SIMM and SJTU. All rights reserved.</rights><rights>Copyright Nature Publishing Group Jul 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4718-734e42713115eaa9a96c4235b0eb612b4b9a201e1a1b1b17cd59b48067309e983</citedby><cites>FETCH-LOGICAL-c4718-734e42713115eaa9a96c4235b0eb612b4b9a201e1a1b1b17cd59b48067309e983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84127X/84127X.jpg</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18478160$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Jing‐Wen</creatorcontrib><creatorcontrib>Wang, Ru‐Yao</creatorcontrib><creatorcontrib>Guo, Qiang‐Su</creatorcontrib><creatorcontrib>Xu, Chen</creatorcontrib><title>Expression of the retinoic acid-metabolizing enzymes RALDH2 and CYP26b1 during mouse postnatal testis development</title><title>Asian journal of andrology</title><addtitle>Asian Journal of Andrology</addtitle><description>Aim： To study the expression pattern of the retinoic acid metabolizing enzymes RALDH2 and CYP26bl during mouse postnatal testis development at both mRNA and protein levels. Methods： Real-time polymerase chain reaction and Western blot analysis were performed to determine the relative quantity of RALDH2 and CYP26bl at both mRNA and protein levels at postnatal day 1, 5, 10, 20, and in adult mice （70 days testes）. Testicular localization of RALDH2 and CYP26b 1 during mouse postnatal development was examined using immunohistochemistry assay. Results： Aldhla2 transcripts and its protein RALDH2 began to increase at postnatal day 10, and remained at a high level through postnatal day 20 to adulthood. Cyp2661 transcripts and CYP26bl protein did not change significantly during mouse postnatal testis development. RALDH2 was undetectable in the postnatal 1, 5 and 10 day testes using immunohis- tochemistry assay. At postnatal day 20 it was detected in pachytene spermatocytes. Robust expression of RALDH2 was restricted in round spermatids in the adult mouse testis. In the developing and adult testis, CYP26bl protein was confined to the peritubular myoepithelial cells. Conclusion： Our results indicate that following birth, the level of retinoic acid in the seminiferous tubules might begin to increase at postnatal day 10, and maintain a high level through postnatal day 20 to adulthood.</description><subject>Aldehyde Oxidoreductases - genetics</subject><subject>Aldehyde Oxidoreductases - metabolism</subject><subject>Animals</subject><subject>CYP26b1</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Rabbits</subject><subject>RALDH2</subject><subject>retinoic acid</subject><subject>Retinoic Acid 4-Hydroxylase</subject><subject>RNA, Messenger - metabolism</subject><subject>Seminiferous Epithelium - cytology</subject><subject>Seminiferous Epithelium - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Spermatids - cytology</subject><subject>Spermatids - metabolism</subject><subject>spermatogenesis</subject><subject>testis</subject><subject>Testis - cytology</subject><subject>Testis - growth & development</subject><subject>Testis - metabolism</subject><subject>出生后睾丸发育</subject><subject>类维生素A酸</subject><subject>精子发生</subject><issn>1008-682X</issn><issn>1745-7262</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkcGO0zAQhiMEYpeFV0AWB24pHtuJ4wOHqruwoAoQAglOlp1Md10SO40TaPfpcWjFSpywJc9I882v8fxZRoAuIJ1X2wVIUeSSlWzBKK0WlIr07h9k538LD1OeSnlZsW9n2ZMYt5QyDko9zs6gErKCkp5nu6t9P2CMLngSNmS8RTLg6HxwNTG1a_IOR2ND6-6cvyHo7w4dRvJ5ub68ZsT4hqy-f2KlBdJMw0x0YYpI-hBHb0bTkhHj6CJp8Ce2oe_Qj0-zRxvTRnx2ihfZ1zdXX1bX-frj23er5TqvhYQql1ygYBI4QIHGKKPKWjBeWIq2BGaFVYZRQDBg05V1UygrKlpKThWqil9kL4-6_RB2UxpDdy7W2LbGYxpSSyp5ATCDL_4Bt2EafJpNM-AFlVTxBFVHqB5CjANudD-4zgwHDVTPnuitnlev59Xr2RP9xxO9T63PT_qT7bC5bzyZkIDXR-CXa_Hw38J6-X75IWX3H6hvg7_ZJRu0NfWPTVLTTKqyqBL0GxuspQg</recordid><startdate>200807</startdate><enddate>200807</enddate><creator>Wu, Jing‐Wen</creator><creator>Wang, Ru‐Yao</creator><creator>Guo, Qiang‐Su</creator><creator>Xu, Chen</creator><general>Blackwell Publishing Ltd</general><general>Medknow Publications & Media Pvt. 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Methods： Real-time polymerase chain reaction and Western blot analysis were performed to determine the relative quantity of RALDH2 and CYP26bl at both mRNA and protein levels at postnatal day 1, 5, 10, 20, and in adult mice （70 days testes）. Testicular localization of RALDH2 and CYP26b 1 during mouse postnatal development was examined using immunohistochemistry assay. Results： Aldhla2 transcripts and its protein RALDH2 began to increase at postnatal day 10, and remained at a high level through postnatal day 20 to adulthood. Cyp2661 transcripts and CYP26bl protein did not change significantly during mouse postnatal testis development. RALDH2 was undetectable in the postnatal 1, 5 and 10 day testes using immunohis- tochemistry assay. At postnatal day 20 it was detected in pachytene spermatocytes. Robust expression of RALDH2 was restricted in round spermatids in the adult mouse testis. In the developing and adult testis, CYP26bl protein was confined to the peritubular myoepithelial cells. Conclusion： Our results indicate that following birth, the level of retinoic acid in the seminiferous tubules might begin to increase at postnatal day 10, and maintain a high level through postnatal day 20 to adulthood.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18478160</pmid><doi>10.1111/j.1745-7262.2008.00408.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aldehyde Oxidoreductases - genetics Aldehyde Oxidoreductases - metabolism Animals CYP26b1 Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Gene Expression Regulation, Developmental Male Mice Mice, Inbred BALB C Rabbits RALDH2 retinoic acid Retinoic Acid 4-Hydroxylase RNA, Messenger - metabolism Seminiferous Epithelium - cytology Seminiferous Epithelium - metabolism Sensitivity and Specificity Spermatids - cytology Spermatids - metabolism spermatogenesis testis Testis - cytology Testis - growth & development Testis - metabolism 出生后睾丸发育 类维生素A酸 精子发生 |
| title | Expression of the retinoic acid-metabolizing enzymes RALDH2 and CYP26b1 during mouse postnatal testis development |
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