Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electrospray ionization mass spectrometry

A novel monolithic enzymatic microreactor was prepared in the fused-silica capillary by in situ polymerization of acrylamide (AA), N-acryloxysuccinimide (NAS) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, which could offer very low ba...

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Veröffentlicht in:	Journal of Chromatography A 2006-02, Vol.1106 (1), p.165-174
Hauptverfasser:	Duan, Jicheng, Sun, Liangliang, Liang, Zhen, Zhang, Jie, Wang, Hui, Zhang, Lihua, Zhang, Weibing, Zhang, Yukui
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Bioreactors Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Electrophoresis, Capillary Enzymatic reactor Enzymes - chemistry Exact sciences and technology Microscopy, Electron, Scanning Monolithic column Nano-liquid chromatography-tandem mass spectrometry Nanotechnology Other chromatographic methods Protein identification Proteins - chemistry Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:	Volltext
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