New Experimental Method to Study Acid/Base Transporters and Their Regulation in Lacrimal Gland Ductal Epithelia
The main function of the lacrimal gland is to produce the most aqueous component of the tear film covering the surfaces of the cornea and the conjunctiva. Studies have been conducted that characterize the mixed fluid and protein secretion of isolated acini, but no methods have been developed to char...
Gespeichert in:
| Veröffentlicht in: | Investigative ophthalmology & visual science 2007-08, Vol.48 (8), p.3746-3755 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3755 |
|---|---|
| container_issue | 8 |
| container_start_page | 3746 |
| container_title | Investigative ophthalmology & visual science |
| container_volume | 48 |
| creator | Toth-Molnar, Edit Venglovecz, Viktoria Ozsvari, Bela Rakonczay, Zoltan, Jr Varro, Andras Papp, Julius G Toth, Andras Lonovics, Janos Takacs, Tamas Ignath, Imre Ivanyi, Bela Hegyi, Peter |
| description | The main function of the lacrimal gland is to produce the most aqueous component of the tear film covering the surfaces of the cornea and the conjunctiva. Studies have been conducted that characterize the mixed fluid and protein secretion of isolated acini, but no methods have been developed to characterize lacrimal gland ductal cell (LGDC) secretion. Secretory mechanisms of ductal epithelia may play physiological roles in the maintenance of the standard environments for the cornea and the conjunctiva.
In this study, the authors developed a rapid method to isolate large quantities of intact lacrimal ducts. The preparation of isolated intact lacrimal gland ducts for the first time enabled the performance of real-time functional experiments on cleaned ducts. Electron microscopy and fluorescence measurements were used to evaluate the viability of lacrimal ducts.
Fluorescence measurements showed that LGDCs express functionally active Na(+)/H(+) exchanger (NHE) and Cl(-)/HCO(3)(-) exchanger (AE). Parasympathomimetic stimulation by carbachol stimulated NHE and AE through the elevation of intracellular calcium concentration. This mechanism can play a role in the regulation of ion and water secretion by LGDCs.
The authors have described a lacrimal gland duct isolation technique in which the intact ducts remain viable and the role of duct cells in tear film secretion can be characterized. These data combined with the novel isolation facilitated understanding of the regulation mechanisms of ductal cell secretion at cellular and molecular levels under normal and pathologic conditions. |
| doi_str_mv | 10.1167/iovs.06-1291 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70734595</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70734595</sourcerecordid><originalsourceid>FETCH-LOGICAL-c350t-b0a07bb21811da167abaa6d39a41f1044b8e398288e103bc7e89d435abbc7ab13</originalsourceid><addsrcrecordid>eNpNkEFv1DAQRi0Eokvhxhn5AifSemIndo6lLAVpAQmWs2U7s41RNklth9B_j6OuVE6ew9PT50fIa2AXALW89OOfeMHqAsoGnpANVFVZVFLxp2TDQNQFE0yckRcx_masBCjZc3IGsq5KKeSGjN9wodu_EwZ_xCGZnn7F1I0tTSP9meb2nl45315-MBHpPpghTmNIGCI1Q0v3HfpAf-Dt3Jvkx4H6ge6My6rsuelX5OPsVul28qnD3puX5NnB9BFfnd5z8uvTdn_9udh9v_lyfbUrHK9YKiwzTFpbggJoTf6mscbULW-MgAMwIaxC3qhSKQTGrZOomlbwyth8Gwv8nLx78E5hvJsxJn300WGfR-E4Ry2Z5KJqqgy-fwBdGGMMeNDTuj_ca2B6DazXwJrVeg2c8Tcn72yP2D7Cp6IZeHsCTHSmP-RmzsdHTjV1CeV_Azt_2y0-oI65Wp-1oJdlEUorzaWo-T87b5HX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70734595</pqid></control><display><type>article</type><title>New Experimental Method to Study Acid/Base Transporters and Their Regulation in Lacrimal Gland Ductal Epithelia</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Toth-Molnar, Edit ; Venglovecz, Viktoria ; Ozsvari, Bela ; Rakonczay, Zoltan, Jr ; Varro, Andras ; Papp, Julius G ; Toth, Andras ; Lonovics, Janos ; Takacs, Tamas ; Ignath, Imre ; Ivanyi, Bela ; Hegyi, Peter</creator><creatorcontrib>Toth-Molnar, Edit ; Venglovecz, Viktoria ; Ozsvari, Bela ; Rakonczay, Zoltan, Jr ; Varro, Andras ; Papp, Julius G ; Toth, Andras ; Lonovics, Janos ; Takacs, Tamas ; Ignath, Imre ; Ivanyi, Bela ; Hegyi, Peter</creatorcontrib><description>The main function of the lacrimal gland is to produce the most aqueous component of the tear film covering the surfaces of the cornea and the conjunctiva. Studies have been conducted that characterize the mixed fluid and protein secretion of isolated acini, but no methods have been developed to characterize lacrimal gland ductal cell (LGDC) secretion. Secretory mechanisms of ductal epithelia may play physiological roles in the maintenance of the standard environments for the cornea and the conjunctiva.
In this study, the authors developed a rapid method to isolate large quantities of intact lacrimal ducts. The preparation of isolated intact lacrimal gland ducts for the first time enabled the performance of real-time functional experiments on cleaned ducts. Electron microscopy and fluorescence measurements were used to evaluate the viability of lacrimal ducts.
Fluorescence measurements showed that LGDCs express functionally active Na(+)/H(+) exchanger (NHE) and Cl(-)/HCO(3)(-) exchanger (AE). Parasympathomimetic stimulation by carbachol stimulated NHE and AE through the elevation of intracellular calcium concentration. This mechanism can play a role in the regulation of ion and water secretion by LGDCs.
The authors have described a lacrimal gland duct isolation technique in which the intact ducts remain viable and the role of duct cells in tear film secretion can be characterized. These data combined with the novel isolation facilitated understanding of the regulation mechanisms of ductal cell secretion at cellular and molecular levels under normal and pathologic conditions.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.06-1291</identifier><identifier>PMID: 17652747</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Acid-Base Equilibrium - physiology ; Acids - metabolism ; Alkalies - metabolism ; Animals ; Biological and medical sciences ; Calcium Signaling - drug effects ; Calcium Signaling - physiology ; Carbachol - pharmacology ; Chloride-Bicarbonate Antiporters - metabolism ; Epithelium - drug effects ; Epithelium - metabolism ; Epithelium - ultrastructure ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Lacrimal Apparatus - drug effects ; Lacrimal Apparatus - metabolism ; Lacrimal Apparatus - ultrastructure ; Male ; Microscopy, Electron, Transmission ; Organ Culture Techniques - methods ; Parasympathomimetics - pharmacology ; Rabbits ; Sodium-Bicarbonate Symporters - metabolism ; Sodium-Hydrogen Exchangers - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Investigative ophthalmology & visual science, 2007-08, Vol.48 (8), p.3746-3755</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-b0a07bb21811da167abaa6d39a41f1044b8e398288e103bc7e89d435abbc7ab13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18962121$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17652747$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toth-Molnar, Edit</creatorcontrib><creatorcontrib>Venglovecz, Viktoria</creatorcontrib><creatorcontrib>Ozsvari, Bela</creatorcontrib><creatorcontrib>Rakonczay, Zoltan, Jr</creatorcontrib><creatorcontrib>Varro, Andras</creatorcontrib><creatorcontrib>Papp, Julius G</creatorcontrib><creatorcontrib>Toth, Andras</creatorcontrib><creatorcontrib>Lonovics, Janos</creatorcontrib><creatorcontrib>Takacs, Tamas</creatorcontrib><creatorcontrib>Ignath, Imre</creatorcontrib><creatorcontrib>Ivanyi, Bela</creatorcontrib><creatorcontrib>Hegyi, Peter</creatorcontrib><title>New Experimental Method to Study Acid/Base Transporters and Their Regulation in Lacrimal Gland Ductal Epithelia</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>The main function of the lacrimal gland is to produce the most aqueous component of the tear film covering the surfaces of the cornea and the conjunctiva. Studies have been conducted that characterize the mixed fluid and protein secretion of isolated acini, but no methods have been developed to characterize lacrimal gland ductal cell (LGDC) secretion. Secretory mechanisms of ductal epithelia may play physiological roles in the maintenance of the standard environments for the cornea and the conjunctiva.
In this study, the authors developed a rapid method to isolate large quantities of intact lacrimal ducts. The preparation of isolated intact lacrimal gland ducts for the first time enabled the performance of real-time functional experiments on cleaned ducts. Electron microscopy and fluorescence measurements were used to evaluate the viability of lacrimal ducts.
Fluorescence measurements showed that LGDCs express functionally active Na(+)/H(+) exchanger (NHE) and Cl(-)/HCO(3)(-) exchanger (AE). Parasympathomimetic stimulation by carbachol stimulated NHE and AE through the elevation of intracellular calcium concentration. This mechanism can play a role in the regulation of ion and water secretion by LGDCs.
The authors have described a lacrimal gland duct isolation technique in which the intact ducts remain viable and the role of duct cells in tear film secretion can be characterized. These data combined with the novel isolation facilitated understanding of the regulation mechanisms of ductal cell secretion at cellular and molecular levels under normal and pathologic conditions.</description><subject>Acid-Base Equilibrium - physiology</subject><subject>Acids - metabolism</subject><subject>Alkalies - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium Signaling - drug effects</subject><subject>Calcium Signaling - physiology</subject><subject>Carbachol - pharmacology</subject><subject>Chloride-Bicarbonate Antiporters - metabolism</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - metabolism</subject><subject>Epithelium - ultrastructure</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lacrimal Apparatus - drug effects</subject><subject>Lacrimal Apparatus - metabolism</subject><subject>Lacrimal Apparatus - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Organ Culture Techniques - methods</subject><subject>Parasympathomimetics - pharmacology</subject><subject>Rabbits</subject><subject>Sodium-Bicarbonate Symporters - metabolism</subject><subject>Sodium-Hydrogen Exchangers - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEFv1DAQRi0Eokvhxhn5AifSemIndo6lLAVpAQmWs2U7s41RNklth9B_j6OuVE6ew9PT50fIa2AXALW89OOfeMHqAsoGnpANVFVZVFLxp2TDQNQFE0yckRcx_masBCjZc3IGsq5KKeSGjN9wodu_EwZ_xCGZnn7F1I0tTSP9meb2nl45315-MBHpPpghTmNIGCI1Q0v3HfpAf-Dt3Jvkx4H6ge6My6rsuelX5OPsVul28qnD3puX5NnB9BFfnd5z8uvTdn_9udh9v_lyfbUrHK9YKiwzTFpbggJoTf6mscbULW-MgAMwIaxC3qhSKQTGrZOomlbwyth8Gwv8nLx78E5hvJsxJn300WGfR-E4Ry2Z5KJqqgy-fwBdGGMMeNDTuj_ca2B6DazXwJrVeg2c8Tcn72yP2D7Cp6IZeHsCTHSmP-RmzsdHTjV1CeV_Azt_2y0-oI65Wp-1oJdlEUorzaWo-T87b5HX</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Toth-Molnar, Edit</creator><creator>Venglovecz, Viktoria</creator><creator>Ozsvari, Bela</creator><creator>Rakonczay, Zoltan, Jr</creator><creator>Varro, Andras</creator><creator>Papp, Julius G</creator><creator>Toth, Andras</creator><creator>Lonovics, Janos</creator><creator>Takacs, Tamas</creator><creator>Ignath, Imre</creator><creator>Ivanyi, Bela</creator><creator>Hegyi, Peter</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070801</creationdate><title>New Experimental Method to Study Acid/Base Transporters and Their Regulation in Lacrimal Gland Ductal Epithelia</title><author>Toth-Molnar, Edit ; Venglovecz, Viktoria ; Ozsvari, Bela ; Rakonczay, Zoltan, Jr ; Varro, Andras ; Papp, Julius G ; Toth, Andras ; Lonovics, Janos ; Takacs, Tamas ; Ignath, Imre ; Ivanyi, Bela ; Hegyi, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-b0a07bb21811da167abaa6d39a41f1044b8e398288e103bc7e89d435abbc7ab13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acid-Base Equilibrium - physiology</topic><topic>Acids - metabolism</topic><topic>Alkalies - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium Signaling - drug effects</topic><topic>Calcium Signaling - physiology</topic><topic>Carbachol - pharmacology</topic><topic>Chloride-Bicarbonate Antiporters - metabolism</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - metabolism</topic><topic>Epithelium - ultrastructure</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lacrimal Apparatus - drug effects</topic><topic>Lacrimal Apparatus - metabolism</topic><topic>Lacrimal Apparatus - ultrastructure</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>Organ Culture Techniques - methods</topic><topic>Parasympathomimetics - pharmacology</topic><topic>Rabbits</topic><topic>Sodium-Bicarbonate Symporters - metabolism</topic><topic>Sodium-Hydrogen Exchangers - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toth-Molnar, Edit</creatorcontrib><creatorcontrib>Venglovecz, Viktoria</creatorcontrib><creatorcontrib>Ozsvari, Bela</creatorcontrib><creatorcontrib>Rakonczay, Zoltan, Jr</creatorcontrib><creatorcontrib>Varro, Andras</creatorcontrib><creatorcontrib>Papp, Julius G</creatorcontrib><creatorcontrib>Toth, Andras</creatorcontrib><creatorcontrib>Lonovics, Janos</creatorcontrib><creatorcontrib>Takacs, Tamas</creatorcontrib><creatorcontrib>Ignath, Imre</creatorcontrib><creatorcontrib>Ivanyi, Bela</creatorcontrib><creatorcontrib>Hegyi, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toth-Molnar, Edit</au><au>Venglovecz, Viktoria</au><au>Ozsvari, Bela</au><au>Rakonczay, Zoltan, Jr</au><au>Varro, Andras</au><au>Papp, Julius G</au><au>Toth, Andras</au><au>Lonovics, Janos</au><au>Takacs, Tamas</au><au>Ignath, Imre</au><au>Ivanyi, Bela</au><au>Hegyi, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New Experimental Method to Study Acid/Base Transporters and Their Regulation in Lacrimal Gland Ductal Epithelia</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>48</volume><issue>8</issue><spage>3746</spage><epage>3755</epage><pages>3746-3755</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>The main function of the lacrimal gland is to produce the most aqueous component of the tear film covering the surfaces of the cornea and the conjunctiva. Studies have been conducted that characterize the mixed fluid and protein secretion of isolated acini, but no methods have been developed to characterize lacrimal gland ductal cell (LGDC) secretion. Secretory mechanisms of ductal epithelia may play physiological roles in the maintenance of the standard environments for the cornea and the conjunctiva.
In this study, the authors developed a rapid method to isolate large quantities of intact lacrimal ducts. The preparation of isolated intact lacrimal gland ducts for the first time enabled the performance of real-time functional experiments on cleaned ducts. Electron microscopy and fluorescence measurements were used to evaluate the viability of lacrimal ducts.
Fluorescence measurements showed that LGDCs express functionally active Na(+)/H(+) exchanger (NHE) and Cl(-)/HCO(3)(-) exchanger (AE). Parasympathomimetic stimulation by carbachol stimulated NHE and AE through the elevation of intracellular calcium concentration. This mechanism can play a role in the regulation of ion and water secretion by LGDCs.
The authors have described a lacrimal gland duct isolation technique in which the intact ducts remain viable and the role of duct cells in tear film secretion can be characterized. These data combined with the novel isolation facilitated understanding of the regulation mechanisms of ductal cell secretion at cellular and molecular levels under normal and pathologic conditions.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>17652747</pmid><doi>10.1167/iovs.06-1291</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-0404 |
| ispartof | Investigative ophthalmology & visual science, 2007-08, Vol.48 (8), p.3746-3755 |
| issn | 0146-0404 1552-5783 1552-5783 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70734595 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Acid-Base Equilibrium - physiology Acids - metabolism Alkalies - metabolism Animals Biological and medical sciences Calcium Signaling - drug effects Calcium Signaling - physiology Carbachol - pharmacology Chloride-Bicarbonate Antiporters - metabolism Epithelium - drug effects Epithelium - metabolism Epithelium - ultrastructure Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Lacrimal Apparatus - drug effects Lacrimal Apparatus - metabolism Lacrimal Apparatus - ultrastructure Male Microscopy, Electron, Transmission Organ Culture Techniques - methods Parasympathomimetics - pharmacology Rabbits Sodium-Bicarbonate Symporters - metabolism Sodium-Hydrogen Exchangers - metabolism Vertebrates: nervous system and sense organs |
| title | New Experimental Method to Study Acid/Base Transporters and Their Regulation in Lacrimal Gland Ductal Epithelia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T12%3A28%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=New%20Experimental%20Method%20to%20Study%20Acid/Base%20Transporters%20and%20Their%20Regulation%20in%20Lacrimal%20Gland%20Ductal%20Epithelia&rft.jtitle=Investigative%20ophthalmology%20&%20visual%20science&rft.au=Toth-Molnar,%20Edit&rft.date=2007-08-01&rft.volume=48&rft.issue=8&rft.spage=3746&rft.epage=3755&rft.pages=3746-3755&rft.issn=0146-0404&rft.eissn=1552-5783&rft.coden=IOVSDA&rft_id=info:doi/10.1167/iovs.06-1291&rft_dat=%3Cproquest_cross%3E70734595%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70734595&rft_id=info:pmid/17652747&rfr_iscdi=true |