Interleukin-10 promoter polymorphisms in patients with systemic lupus erythematosus from the Canary Islands
Summary The purpose of this study was to examine whether several allelic variants in the polymorphic interleukin (IL)‐10 promoter region were related with an increased risk of developing systemic lupus erythematosus (SLE) in Spanish patients from Canary Islands. Microsatellites (MS) at positions –40...
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| Veröffentlicht in: | International journal of immunogenetics 2008-06, Vol.35 (3), p.235-242 |
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| creator | Rosado, S. Rua-Figueroa, I. Vargas, J. A. Garcia-Laorden, M. I. Losada-Fernandez, I. Martin-Donaire, T. Perez-Chacon, G. Rodriguez-Gallego, C. Naranjo-Hernandez, A. Ojeda-Bruno, S. Citores, M. J. Perez-Aciego, P. |
| description | Summary
The purpose of this study was to examine whether several allelic variants in the polymorphic interleukin (IL)‐10 promoter region were related with an increased risk of developing systemic lupus erythematosus (SLE) in Spanish patients from Canary Islands. Microsatellites (MS) at positions –4000 and –1200 (IL10R and IL10G, respectively) and single nucleotide polymorphisms (SNPs) (MS) at positions –1082G/A, –819C/T and –592C/A of the IL‐10 promoter were analysed in patients with SLE and healthy controls from Canary Islands (Spain). We found that SNPs but not MS were associated with SLE. The GCC haplotype frequency was significantly higher in SLE patients (0.43) than in healthy donors (0.33) [P = 0.02; OR = 1.50 (95% CI = 1.06–2.14)], whereas the ACC haplotype was less represented in patients (0.28 vs. 0.37) [P = 0.02; OR = 0.64 (95% CI = 0.44–0.92)]. To assess the functional role of genotypes, serum IL‐10 levels from patients and controls were quantified by ELISA. Also, the lipopolysaccharide‐induced IL‐10 secretion by monocytes from healthy controls was evaluated in vitro. Serum IL‐10 levels were higher in patients [median (interquartile range) = 2.8 pg/mL (1.8–4.2)] than in controls [0.9 pg/mL (0–3.5)] (P = 0.02), but no association was observed between serum IL‐10 levels or lipopolysaccharide‐induced IL‐10 secretion and the IL‐10 promoter haplotypes. These data suggest that the IL‐10 promoter haplotype that produces higher levels of cytokine is associated with SLE in patients from Canary Islands. |
| doi_str_mv | 10.1111/j.1744-313X.2008.00762.x |
| format | Article |
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The purpose of this study was to examine whether several allelic variants in the polymorphic interleukin (IL)‐10 promoter region were related with an increased risk of developing systemic lupus erythematosus (SLE) in Spanish patients from Canary Islands. Microsatellites (MS) at positions –4000 and –1200 (IL10R and IL10G, respectively) and single nucleotide polymorphisms (SNPs) (MS) at positions –1082G/A, –819C/T and –592C/A of the IL‐10 promoter were analysed in patients with SLE and healthy controls from Canary Islands (Spain). We found that SNPs but not MS were associated with SLE. The GCC haplotype frequency was significantly higher in SLE patients (0.43) than in healthy donors (0.33) [P = 0.02; OR = 1.50 (95% CI = 1.06–2.14)], whereas the ACC haplotype was less represented in patients (0.28 vs. 0.37) [P = 0.02; OR = 0.64 (95% CI = 0.44–0.92)]. To assess the functional role of genotypes, serum IL‐10 levels from patients and controls were quantified by ELISA. Also, the lipopolysaccharide‐induced IL‐10 secretion by monocytes from healthy controls was evaluated in vitro. Serum IL‐10 levels were higher in patients [median (interquartile range) = 2.8 pg/mL (1.8–4.2)] than in controls [0.9 pg/mL (0–3.5)] (P = 0.02), but no association was observed between serum IL‐10 levels or lipopolysaccharide‐induced IL‐10 secretion and the IL‐10 promoter haplotypes. These data suggest that the IL‐10 promoter haplotype that produces higher levels of cytokine is associated with SLE in patients from Canary Islands.</description><identifier>ISSN: 1744-3121</identifier><identifier>EISSN: 1744-313X</identifier><identifier>DOI: 10.1111/j.1744-313X.2008.00762.x</identifier><identifier>PMID: 18397303</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Alleles ; Cells, Cultured ; Female ; Genetic Predisposition to Disease ; Genotype ; Haplotypes - genetics ; Humans ; Interleukin-10 - blood ; Interleukin-10 - genetics ; Interleukin-10 - metabolism ; Lipopolysaccharides - pharmacology ; Lupus Erythematosus, Systemic - genetics ; Lupus Erythematosus, Systemic - immunology ; Lupus Erythematosus, Systemic - metabolism ; Male ; Microsatellite Repeats ; Monocytes - metabolism ; Polymorphism, Single Nucleotide ; Promoter Regions, Genetic - genetics ; Spain</subject><ispartof>International journal of immunogenetics, 2008-06, Vol.35 (3), p.235-242</ispartof><rights>2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4352-57e6dcd868b94fe90f34f1afacc7233612b03e1c0f0075082c387e103a14b25a3</citedby><cites>FETCH-LOGICAL-c4352-57e6dcd868b94fe90f34f1afacc7233612b03e1c0f0075082c387e103a14b25a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1744-313X.2008.00762.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1744-313X.2008.00762.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18397303$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosado, S.</creatorcontrib><creatorcontrib>Rua-Figueroa, I.</creatorcontrib><creatorcontrib>Vargas, J. A.</creatorcontrib><creatorcontrib>Garcia-Laorden, M. I.</creatorcontrib><creatorcontrib>Losada-Fernandez, I.</creatorcontrib><creatorcontrib>Martin-Donaire, T.</creatorcontrib><creatorcontrib>Perez-Chacon, G.</creatorcontrib><creatorcontrib>Rodriguez-Gallego, C.</creatorcontrib><creatorcontrib>Naranjo-Hernandez, A.</creatorcontrib><creatorcontrib>Ojeda-Bruno, S.</creatorcontrib><creatorcontrib>Citores, M. J.</creatorcontrib><creatorcontrib>Perez-Aciego, P.</creatorcontrib><title>Interleukin-10 promoter polymorphisms in patients with systemic lupus erythematosus from the Canary Islands</title><title>International journal of immunogenetics</title><addtitle>Int J Immunogenet</addtitle><description>Summary
The purpose of this study was to examine whether several allelic variants in the polymorphic interleukin (IL)‐10 promoter region were related with an increased risk of developing systemic lupus erythematosus (SLE) in Spanish patients from Canary Islands. Microsatellites (MS) at positions –4000 and –1200 (IL10R and IL10G, respectively) and single nucleotide polymorphisms (SNPs) (MS) at positions –1082G/A, –819C/T and –592C/A of the IL‐10 promoter were analysed in patients with SLE and healthy controls from Canary Islands (Spain). We found that SNPs but not MS were associated with SLE. The GCC haplotype frequency was significantly higher in SLE patients (0.43) than in healthy donors (0.33) [P = 0.02; OR = 1.50 (95% CI = 1.06–2.14)], whereas the ACC haplotype was less represented in patients (0.28 vs. 0.37) [P = 0.02; OR = 0.64 (95% CI = 0.44–0.92)]. To assess the functional role of genotypes, serum IL‐10 levels from patients and controls were quantified by ELISA. Also, the lipopolysaccharide‐induced IL‐10 secretion by monocytes from healthy controls was evaluated in vitro. Serum IL‐10 levels were higher in patients [median (interquartile range) = 2.8 pg/mL (1.8–4.2)] than in controls [0.9 pg/mL (0–3.5)] (P = 0.02), but no association was observed between serum IL‐10 levels or lipopolysaccharide‐induced IL‐10 secretion and the IL‐10 promoter haplotypes. These data suggest that the IL‐10 promoter haplotype that produces higher levels of cytokine is associated with SLE in patients from Canary Islands.</description><subject>Alleles</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotype</subject><subject>Haplotypes - genetics</subject><subject>Humans</subject><subject>Interleukin-10 - blood</subject><subject>Interleukin-10 - genetics</subject><subject>Interleukin-10 - metabolism</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lupus Erythematosus, Systemic - genetics</subject><subject>Lupus Erythematosus, Systemic - immunology</subject><subject>Lupus Erythematosus, Systemic - metabolism</subject><subject>Male</subject><subject>Microsatellite Repeats</subject><subject>Monocytes - metabolism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Spain</subject><issn>1744-3121</issn><issn>1744-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUclu2zAUJIIGWdz-QsBTb1IeF4k0kEthNKmCbAVa1DeCpimYtraSEmL9fenYcI4pL3yPnBly3iCECaQkrut1SgTnCSNsnlIAmQKInKbbE3RxvPh0rCk5R5chrAFYzjmcoXMi2VQwYBdoUzS99ZUdNq5JCODOt3UbT3DXVmPd-m7lQh2wa3Cne2ebPuBX169wGENva2dwNXRDwNaP_crWum9D7MoogmOPZ7rRfsRFqHSzDJ_RaamrYL8c9gn6ffv91-xH8vB8V8y-PSSGs4wmmbD50ixlLhdTXtoplIyXRJfaGEEZywldALPEQBldZyCpYVJYAkwTvqCZZhP0da8bzfwdbOhV7YKxVfyEbYegBAgqJGcfAuNocyAROUFyDzS-DcHbUnXe1dGaIqB2iai12g1b7Qa_o0n1lojaRurV4Y1hUdvlO_EQQQTc7AGvrrLjfwur4r6IRaQne7qLgWyPdO03KhdMZOrP052a3j_-fJnPbtWc_QNAxqr6</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Rosado, S.</creator><creator>Rua-Figueroa, I.</creator><creator>Vargas, J. A.</creator><creator>Garcia-Laorden, M. I.</creator><creator>Losada-Fernandez, I.</creator><creator>Martin-Donaire, T.</creator><creator>Perez-Chacon, G.</creator><creator>Rodriguez-Gallego, C.</creator><creator>Naranjo-Hernandez, A.</creator><creator>Ojeda-Bruno, S.</creator><creator>Citores, M. J.</creator><creator>Perez-Aciego, P.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>Interleukin-10 promoter polymorphisms in patients with systemic lupus erythematosus from the Canary Islands</title><author>Rosado, S. ; Rua-Figueroa, I. ; Vargas, J. A. ; Garcia-Laorden, M. I. ; Losada-Fernandez, I. ; Martin-Donaire, T. ; Perez-Chacon, G. ; Rodriguez-Gallego, C. ; Naranjo-Hernandez, A. ; Ojeda-Bruno, S. ; Citores, M. J. ; Perez-Aciego, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4352-57e6dcd868b94fe90f34f1afacc7233612b03e1c0f0075082c387e103a14b25a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alleles</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Genetic Predisposition to Disease</topic><topic>Genotype</topic><topic>Haplotypes - genetics</topic><topic>Humans</topic><topic>Interleukin-10 - blood</topic><topic>Interleukin-10 - genetics</topic><topic>Interleukin-10 - metabolism</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lupus Erythematosus, Systemic - genetics</topic><topic>Lupus Erythematosus, Systemic - immunology</topic><topic>Lupus Erythematosus, Systemic - metabolism</topic><topic>Male</topic><topic>Microsatellite Repeats</topic><topic>Monocytes - metabolism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Spain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosado, S.</creatorcontrib><creatorcontrib>Rua-Figueroa, I.</creatorcontrib><creatorcontrib>Vargas, J. A.</creatorcontrib><creatorcontrib>Garcia-Laorden, M. I.</creatorcontrib><creatorcontrib>Losada-Fernandez, I.</creatorcontrib><creatorcontrib>Martin-Donaire, T.</creatorcontrib><creatorcontrib>Perez-Chacon, G.</creatorcontrib><creatorcontrib>Rodriguez-Gallego, C.</creatorcontrib><creatorcontrib>Naranjo-Hernandez, A.</creatorcontrib><creatorcontrib>Ojeda-Bruno, S.</creatorcontrib><creatorcontrib>Citores, M. J.</creatorcontrib><creatorcontrib>Perez-Aciego, P.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of immunogenetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosado, S.</au><au>Rua-Figueroa, I.</au><au>Vargas, J. A.</au><au>Garcia-Laorden, M. I.</au><au>Losada-Fernandez, I.</au><au>Martin-Donaire, T.</au><au>Perez-Chacon, G.</au><au>Rodriguez-Gallego, C.</au><au>Naranjo-Hernandez, A.</au><au>Ojeda-Bruno, S.</au><au>Citores, M. J.</au><au>Perez-Aciego, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interleukin-10 promoter polymorphisms in patients with systemic lupus erythematosus from the Canary Islands</atitle><jtitle>International journal of immunogenetics</jtitle><addtitle>Int J Immunogenet</addtitle><date>2008-06</date><risdate>2008</risdate><volume>35</volume><issue>3</issue><spage>235</spage><epage>242</epage><pages>235-242</pages><issn>1744-3121</issn><eissn>1744-313X</eissn><abstract>Summary
The purpose of this study was to examine whether several allelic variants in the polymorphic interleukin (IL)‐10 promoter region were related with an increased risk of developing systemic lupus erythematosus (SLE) in Spanish patients from Canary Islands. Microsatellites (MS) at positions –4000 and –1200 (IL10R and IL10G, respectively) and single nucleotide polymorphisms (SNPs) (MS) at positions –1082G/A, –819C/T and –592C/A of the IL‐10 promoter were analysed in patients with SLE and healthy controls from Canary Islands (Spain). We found that SNPs but not MS were associated with SLE. The GCC haplotype frequency was significantly higher in SLE patients (0.43) than in healthy donors (0.33) [P = 0.02; OR = 1.50 (95% CI = 1.06–2.14)], whereas the ACC haplotype was less represented in patients (0.28 vs. 0.37) [P = 0.02; OR = 0.64 (95% CI = 0.44–0.92)]. To assess the functional role of genotypes, serum IL‐10 levels from patients and controls were quantified by ELISA. Also, the lipopolysaccharide‐induced IL‐10 secretion by monocytes from healthy controls was evaluated in vitro. Serum IL‐10 levels were higher in patients [median (interquartile range) = 2.8 pg/mL (1.8–4.2)] than in controls [0.9 pg/mL (0–3.5)] (P = 0.02), but no association was observed between serum IL‐10 levels or lipopolysaccharide‐induced IL‐10 secretion and the IL‐10 promoter haplotypes. These data suggest that the IL‐10 promoter haplotype that produces higher levels of cytokine is associated with SLE in patients from Canary Islands.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18397303</pmid><doi>10.1111/j.1744-313X.2008.00762.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Alleles Cells, Cultured Female Genetic Predisposition to Disease Genotype Haplotypes - genetics Humans Interleukin-10 - blood Interleukin-10 - genetics Interleukin-10 - metabolism Lipopolysaccharides - pharmacology Lupus Erythematosus, Systemic - genetics Lupus Erythematosus, Systemic - immunology Lupus Erythematosus, Systemic - metabolism Male Microsatellite Repeats Monocytes - metabolism Polymorphism, Single Nucleotide Promoter Regions, Genetic - genetics Spain |
| title | Interleukin-10 promoter polymorphisms in patients with systemic lupus erythematosus from the Canary Islands |
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