GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines
Two Brassica napus--Crambe abyssinica monosomic addition lines (2n=39, AACC plus a single chromosome from C. abyssinca) were obtained from the F2 progeny of the asymmetric somatic hybrid. The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridi...
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| Veröffentlicht in: | Plant cell reports 2006-02, Vol.25 (1), p.35-40 |
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| creator | Wang, Y Sonntag, K Rudloff, E Wehling, P Snowdon, R.J |
| description | Two Brassica napus--Crambe abyssinica monosomic addition lines (2n=39, AACC plus a single chromosome from C. abyssinca) were obtained from the F2 progeny of the asymmetric somatic hybrid. The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridization (GISH). Twenty-seven microspore-derived plants from the addition lines were obtained. Fourteen seedlings were determined to be diploid plants (2n=38) arising from spontaneous chromosome doubling, while 13 seedlings were confirmed as haploid plants. Doubled haploid plants produced after treatment with colchicine and two disomic chromosome addition lines (2n=40, AACC plus a single pair of homologous chromosomes from C. abyssinca) could again be identified by GISH analysis. The lines are potentially useful for molecular genetic analysis of novel C. abyssinica genes or alleles contributing to traits relevant for oilseed rape (B. napus) breeding. |
| doi_str_mv | 10.1007/s00299-005-0031-3 |
| format | Article |
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The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridization (GISH). Twenty-seven microspore-derived plants from the addition lines were obtained. Fourteen seedlings were determined to be diploid plants (2n=38) arising from spontaneous chromosome doubling, while 13 seedlings were confirmed as haploid plants. Doubled haploid plants produced after treatment with colchicine and two disomic chromosome addition lines (2n=40, AACC plus a single pair of homologous chromosomes from C. abyssinca) could again be identified by GISH analysis. The lines are potentially useful for molecular genetic analysis of novel C. abyssinica genes or alleles contributing to traits relevant for oilseed rape (B. napus) breeding.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-005-0031-3</identifier><identifier>PMID: 16151813</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Biological and medical sciences ; Biotechnology ; Brassica napus ; Brassica napus - anatomy & histology ; Brassica napus - genetics ; chromosomes ; Chromosomes, Plant - genetics ; Crambe abyssinica ; Crambe Plant - genetics ; Culture Techniques ; Cytological Techniques ; diploidy ; disomics ; erucic acid ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Genome, Plant - genetics ; genomic in situ hybridization ; haploidy ; herbaceous plants ; In Situ Hybridization ; lipid content ; Methods. Procedures. Technologies ; micropropagation ; microspores ; Miscellaneous ; monosomics ; Monosomy - genetics ; oil crops ; Plant cells and fungal cells ; plant genetics ; Ploidies ; somatic embryos ; Spores - cytology ; Spores - growth & development ; vegetable crops</subject><ispartof>Plant cell reports, 2006-02, Vol.25 (1), p.35-40</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-c249a8b1b7de26b630a53d0bd22208a5799eadc1b0970b13db01d7730c961d553</citedby><cites>FETCH-LOGICAL-c384t-c249a8b1b7de26b630a53d0bd22208a5799eadc1b0970b13db01d7730c961d553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17471144$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16151813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Y</creatorcontrib><creatorcontrib>Sonntag, K</creatorcontrib><creatorcontrib>Rudloff, E</creatorcontrib><creatorcontrib>Wehling, P</creatorcontrib><creatorcontrib>Snowdon, R.J</creatorcontrib><title>GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Two Brassica napus--Crambe abyssinica monosomic addition lines (2n=39, AACC plus a single chromosome from C. abyssinca) were obtained from the F2 progeny of the asymmetric somatic hybrid. The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridization (GISH). Twenty-seven microspore-derived plants from the addition lines were obtained. Fourteen seedlings were determined to be diploid plants (2n=38) arising from spontaneous chromosome doubling, while 13 seedlings were confirmed as haploid plants. Doubled haploid plants produced after treatment with colchicine and two disomic chromosome addition lines (2n=40, AACC plus a single pair of homologous chromosomes from C. abyssinca) could again be identified by GISH analysis. The lines are potentially useful for molecular genetic analysis of novel C. abyssinica genes or alleles contributing to traits relevant for oilseed rape (B. napus) breeding.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Brassica napus</subject><subject>Brassica napus - anatomy & histology</subject><subject>Brassica napus - genetics</subject><subject>chromosomes</subject><subject>Chromosomes, Plant - genetics</subject><subject>Crambe abyssinica</subject><subject>Crambe Plant - genetics</subject><subject>Culture Techniques</subject><subject>Cytological Techniques</subject><subject>diploidy</subject><subject>disomics</subject><subject>erucic acid</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genome, Plant - genetics</subject><subject>genomic in situ hybridization</subject><subject>haploidy</subject><subject>herbaceous plants</subject><subject>In Situ Hybridization</subject><subject>lipid content</subject><subject>Methods. Procedures. Technologies</subject><subject>micropropagation</subject><subject>microspores</subject><subject>Miscellaneous</subject><subject>monosomics</subject><subject>Monosomy - genetics</subject><subject>oil crops</subject><subject>Plant cells and fungal cells</subject><subject>plant genetics</subject><subject>Ploidies</subject><subject>somatic embryos</subject><subject>Spores - cytology</subject><subject>Spores - growth & development</subject><subject>vegetable crops</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQxi0EokvhAbiAL3ALzNhOvDnSFbSVKnEolbhZ4z8BoyRe7M1h34JHxtGuVHHiYI30-Tff2P7M2GuEDwigPxYA0fcNQFuXxEY-YRtUUjQC5PenbANaYKM1qgv2opRfAHVTd8_ZBXbY4hblhv25vr2_4TTTeCyx8DRwH0uaouNXmUqJjvhM-6U0u0yTDZzssarzqrufOU2pwlX1Ph5imvkY51D4Pie_uOC5PfJqlVPZpxy4W8bDUutQ-_iU5nQa9G_zS_ZsoLGEV-d6yR6-fP62u2nuvl7f7j7dNU5u1aFxQvW0tWi1D6KznQRqpQfrhRCwpVb3fSDv0EKvwaL0FtBrLcH1Hfq2lZfs_cm3Hvb3EsrBTLG4MI40h7QUo-vjtUrI_4LYC90p3VUQT-B64ZLDYPY5TpSPBsGseZlTXqbmZda8zGr-5my-2Cn4x45zQBV4dwaoOBqHTLOL5ZHTSiMqVbm3J26gZOhHrszDvQCUgNCpvn6Iv1U-qR4</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Wang, Y</creator><creator>Sonntag, K</creator><creator>Rudloff, E</creator><creator>Wehling, P</creator><creator>Snowdon, R.J</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060201</creationdate><title>GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines</title><author>Wang, Y ; Sonntag, K ; Rudloff, E ; Wehling, P ; Snowdon, R.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-c249a8b1b7de26b630a53d0bd22208a5799eadc1b0970b13db01d7730c961d553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Brassica napus</topic><topic>Brassica napus - anatomy & histology</topic><topic>Brassica napus - genetics</topic><topic>chromosomes</topic><topic>Chromosomes, Plant - genetics</topic><topic>Crambe abyssinica</topic><topic>Crambe Plant - genetics</topic><topic>Culture Techniques</topic><topic>Cytological Techniques</topic><topic>diploidy</topic><topic>disomics</topic><topic>erucic acid</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genome, Plant - genetics</topic><topic>genomic in situ hybridization</topic><topic>haploidy</topic><topic>herbaceous plants</topic><topic>In Situ Hybridization</topic><topic>lipid content</topic><topic>Methods. Procedures. Technologies</topic><topic>micropropagation</topic><topic>microspores</topic><topic>Miscellaneous</topic><topic>monosomics</topic><topic>Monosomy - genetics</topic><topic>oil crops</topic><topic>Plant cells and fungal cells</topic><topic>plant genetics</topic><topic>Ploidies</topic><topic>somatic embryos</topic><topic>Spores - cytology</topic><topic>Spores - growth & development</topic><topic>vegetable crops</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Y</creatorcontrib><creatorcontrib>Sonntag, K</creatorcontrib><creatorcontrib>Rudloff, E</creatorcontrib><creatorcontrib>Wehling, P</creatorcontrib><creatorcontrib>Snowdon, R.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Y</au><au>Sonntag, K</au><au>Rudloff, E</au><au>Wehling, P</au><au>Snowdon, R.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>25</volume><issue>1</issue><spage>35</spage><epage>40</epage><pages>35-40</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Two Brassica napus--Crambe abyssinica monosomic addition lines (2n=39, AACC plus a single chromosome from C. abyssinca) were obtained from the F2 progeny of the asymmetric somatic hybrid. The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridization (GISH). Twenty-seven microspore-derived plants from the addition lines were obtained. Fourteen seedlings were determined to be diploid plants (2n=38) arising from spontaneous chromosome doubling, while 13 seedlings were confirmed as haploid plants. Doubled haploid plants produced after treatment with colchicine and two disomic chromosome addition lines (2n=40, AACC plus a single pair of homologous chromosomes from C. abyssinca) could again be identified by GISH analysis. The lines are potentially useful for molecular genetic analysis of novel C. abyssinica genes or alleles contributing to traits relevant for oilseed rape (B. napus) breeding.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>16151813</pmid><doi>10.1007/s00299-005-0031-3</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Biotechnology Brassica napus Brassica napus - anatomy & histology Brassica napus - genetics chromosomes Chromosomes, Plant - genetics Crambe abyssinica Crambe Plant - genetics Culture Techniques Cytological Techniques diploidy disomics erucic acid Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Genome, Plant - genetics genomic in situ hybridization haploidy herbaceous plants In Situ Hybridization lipid content Methods. Procedures. Technologies micropropagation microspores Miscellaneous monosomics Monosomy - genetics oil crops Plant cells and fungal cells plant genetics Ploidies somatic embryos Spores - cytology Spores - growth & development vegetable crops |
| title | GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines |
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