Recombination reaction of rhodopsin in situ studied by photoconversion of “indicator yellow”

We measured the kinetics of recombination of 11- cis-retinal with opsin in intact frog rod outer segment (ROS). The rhodopsin in ROS was bleached and allowed to decay to “indicator yellow,” a photoproduct where all- trans-retinal is partly free, and partly bound to non-specific amino groups of disk...

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Veröffentlicht in:	Vision research (Oxford) 2006-05, Vol.46 (10), p.1665-1675
Hauptverfasser:	Kolesnikov, A.V., Shukolyukov, S.A., Cornwall, M.C., Govardovskii, V.I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Indicator yellow Photic Stimulation - methods Photoregeneration Rana temporaria Recombination Retinal Pigments - metabolism Retinaldehyde - metabolism Rhodopsin - biosynthesis Rhodopsin regeneration Rod Cell Outer Segment - metabolism Rod Opsins - metabolism Tissue Culture Techniques Visual cycle
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