Capillary electrophoresis versus differential scanning calorimetry for the analysis of free enzyme versus enzyme-ligand complexes: In the search of the ligand-free status of cholinesterases

Cholinesterases (ChEs) are highly efficient biocatalysts whose active site is buried in a deep, narrow gorge. The talent of CE to discover inhibitors in the gorge of highly purified preparations has fairly altered the meaning of a ChE ligand‐free status. To attempt at a description of this one, we i...

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Veröffentlicht in:	Electrophoresis 2006-02, Vol.27 (2), p.442-451
Hauptverfasser:	Rochu, Daniel, Cléry-Barraud, Cécile, Renault, Frédérique, Chevalier, Aurélie, Bon, Cassian, Masson, Patrick
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholinesterase - chemistry Animals Binding Sites Bungarus - metabolism Calorimetry, Differential Scanning Capillary electrophoresis Cholinesterase Cholinesterase Inhibitors - chemistry Differential scanning calorimetry Electrophoresis, Capillary Ligand-free Ligands Models, Molecular Thermodynamics
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