High frequency ultrasound imaging of a single-species biofilm
Abstract Objective This study evaluated the feasibility of a high frequency ultrasound scan to examine the 3D morphology of Streptococcus mutans biofilms grown in vitro. Methods Six 2-day S. mutans biofilms and six 7-day biofilms were grown on tissue culture membranes and on bovine dentine discs. A...
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| Veröffentlicht in: | Journal of dentistry 2007-08, Vol.35 (8), p.673-678 |
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| container_title | Journal of dentistry |
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| creator | Shemesh, H Goertz, D.E van der Sluis, L.W.M de Jong, N Wu, M.K Wesselink, P.R |
| description | Abstract Objective This study evaluated the feasibility of a high frequency ultrasound scan to examine the 3D morphology of Streptococcus mutans biofilms grown in vitro. Methods Six 2-day S. mutans biofilms and six 7-day biofilms were grown on tissue culture membranes and on bovine dentine discs. A sterile growth medium on the membrane and disc were used as controls. Surfaces were rinsed and then immersed in sterile saline. High-frequency ultrasound imaging system was used to scan these surfaces at 55 MHz, and a computer program calculated the average thickness of the biofilm layer from the 3D images. Results 3D pictures of the biofilm layers were obtained. Different cross-sections and plains are easily demonstrated. The average thickness of the 7-day biofilm was significantly bigger than the 2-day on both the membranes and dentinal discs. No structures were observed on the sterile membrane or disc. Conclusion Three-dimensional structural imaging in situ is possible without damaging the biofilm layer in a quick and easy manner and can therefore be used to evaluate biofilms longitudinally as a function of time. |
| doi_str_mv | 10.1016/j.jdent.2007.05.007 |
| format | Article |
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Methods Six 2-day S. mutans biofilms and six 7-day biofilms were grown on tissue culture membranes and on bovine dentine discs. A sterile growth medium on the membrane and disc were used as controls. Surfaces were rinsed and then immersed in sterile saline. High-frequency ultrasound imaging system was used to scan these surfaces at 55 MHz, and a computer program calculated the average thickness of the biofilm layer from the 3D images. Results 3D pictures of the biofilm layers were obtained. Different cross-sections and plains are easily demonstrated. The average thickness of the 7-day biofilm was significantly bigger than the 2-day on both the membranes and dentinal discs. No structures were observed on the sterile membrane or disc. Conclusion Three-dimensional structural imaging in situ is possible without damaging the biofilm layer in a quick and easy manner and can therefore be used to evaluate biofilms longitudinally as a function of time.</description><identifier>ISSN: 0300-5712</identifier><identifier>EISSN: 1879-176X</identifier><identifier>DOI: 10.1016/j.jdent.2007.05.007</identifier><identifier>PMID: 17604896</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Biofilm ; Biofilms ; Biofilms - growth & development ; Cattle ; Dentin - diagnostic imaging ; Dentin - microbiology ; Dentistry ; Feasibility Studies ; Imaging ; Methods ; Microbiology ; NMR ; Nuclear magnetic resonance ; Signal processing ; Streptococcus mutans - physiology ; Studies ; Sucrose ; Time Factors ; Ultrasonography - methods ; Ultrasound</subject><ispartof>Journal of dentistry, 2007-08, Vol.35 (8), p.673-678</ispartof><rights>Elsevier Ltd</rights><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-bc920bc017e6c27dc718acad5abf7efee88f7384c5d0f97bebfc3a01dc58b0133</citedby><cites>FETCH-LOGICAL-c440t-bc920bc017e6c27dc718acad5abf7efee88f7384c5d0f97bebfc3a01dc58b0133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jdent.2007.05.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17604896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shemesh, H</creatorcontrib><creatorcontrib>Goertz, D.E</creatorcontrib><creatorcontrib>van der Sluis, L.W.M</creatorcontrib><creatorcontrib>de Jong, N</creatorcontrib><creatorcontrib>Wu, M.K</creatorcontrib><creatorcontrib>Wesselink, P.R</creatorcontrib><title>High frequency ultrasound imaging of a single-species biofilm</title><title>Journal of dentistry</title><addtitle>J Dent</addtitle><description>Abstract Objective This study evaluated the feasibility of a high frequency ultrasound scan to examine the 3D morphology of Streptococcus mutans biofilms grown in vitro. Methods Six 2-day S. mutans biofilms and six 7-day biofilms were grown on tissue culture membranes and on bovine dentine discs. A sterile growth medium on the membrane and disc were used as controls. Surfaces were rinsed and then immersed in sterile saline. High-frequency ultrasound imaging system was used to scan these surfaces at 55 MHz, and a computer program calculated the average thickness of the biofilm layer from the 3D images. Results 3D pictures of the biofilm layers were obtained. Different cross-sections and plains are easily demonstrated. The average thickness of the 7-day biofilm was significantly bigger than the 2-day on both the membranes and dentinal discs. No structures were observed on the sterile membrane or disc. Conclusion Three-dimensional structural imaging in situ is possible without damaging the biofilm layer in a quick and easy manner and can therefore be used to evaluate biofilms longitudinally as a function of time.</description><subject>Animals</subject><subject>Biofilm</subject><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Cattle</subject><subject>Dentin - diagnostic imaging</subject><subject>Dentin - microbiology</subject><subject>Dentistry</subject><subject>Feasibility Studies</subject><subject>Imaging</subject><subject>Methods</subject><subject>Microbiology</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Signal processing</subject><subject>Streptococcus mutans - physiology</subject><subject>Studies</subject><subject>Sucrose</subject><subject>Time Factors</subject><subject>Ultrasonography - methods</subject><subject>Ultrasound</subject><issn>0300-5712</issn><issn>1879-176X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpabZJf0EgGAq52R35S_IhgRLSpBDIoQ30JuTRaCPHa28ku7D_PnJ2IZBLTyPQM9K8zzB2yiHjwOvvXdYZGqYsBxAZVFksH9iKS9GkXNR_P7IVFABpJXh-xL6E0AFACXnzmR3FeyhlU6_Yxa1bPybW0_NMA-6SuZ-8DuM8mMRt9NoN62S0iU5CPPWUhi2ho5C0brSu35ywT1b3gb4e6jF7-Hn95-o2vbu_-XX14y7FsoQpbbHJoUXggmrMhUHBpUZtKt1aQZZISisKWWJlwDaipdZioYEbrGQLvCiO2fn-3a0f46BhUhsXkPpeDzTOQQkQOY-hIvjtHdiNsx_ibIovNqTknEeq2FPoxxA8WbX1Ma3fRUgtblWnXt2qxa2CSsUSu84Ob8_thsxbz0FmBC72AEUV_xx5FaKsAck4TzgpM7r_fHD5rh97NzjU_RPtKLwlUSFXoH4vgZbtgoibbeq6eAHmbKBu</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Shemesh, H</creator><creator>Goertz, D.E</creator><creator>van der Sluis, L.W.M</creator><creator>de Jong, N</creator><creator>Wu, M.K</creator><creator>Wesselink, P.R</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20070801</creationdate><title>High frequency ultrasound imaging of a single-species biofilm</title><author>Shemesh, H ; Goertz, D.E ; van der Sluis, L.W.M ; de Jong, N ; Wu, M.K ; Wesselink, P.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-bc920bc017e6c27dc718acad5abf7efee88f7384c5d0f97bebfc3a01dc58b0133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biofilm</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Cattle</topic><topic>Dentin - diagnostic imaging</topic><topic>Dentin - microbiology</topic><topic>Dentistry</topic><topic>Feasibility Studies</topic><topic>Imaging</topic><topic>Methods</topic><topic>Microbiology</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Signal processing</topic><topic>Streptococcus mutans - physiology</topic><topic>Studies</topic><topic>Sucrose</topic><topic>Time Factors</topic><topic>Ultrasonography - methods</topic><topic>Ultrasound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shemesh, H</creatorcontrib><creatorcontrib>Goertz, D.E</creatorcontrib><creatorcontrib>van der Sluis, L.W.M</creatorcontrib><creatorcontrib>de Jong, N</creatorcontrib><creatorcontrib>Wu, M.K</creatorcontrib><creatorcontrib>Wesselink, P.R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shemesh, H</au><au>Goertz, D.E</au><au>van der Sluis, L.W.M</au><au>de Jong, N</au><au>Wu, M.K</au><au>Wesselink, P.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High frequency ultrasound imaging of a single-species biofilm</atitle><jtitle>Journal of dentistry</jtitle><addtitle>J Dent</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>35</volume><issue>8</issue><spage>673</spage><epage>678</epage><pages>673-678</pages><issn>0300-5712</issn><eissn>1879-176X</eissn><abstract>Abstract Objective This study evaluated the feasibility of a high frequency ultrasound scan to examine the 3D morphology of Streptococcus mutans biofilms grown in vitro. Methods Six 2-day S. mutans biofilms and six 7-day biofilms were grown on tissue culture membranes and on bovine dentine discs. A sterile growth medium on the membrane and disc were used as controls. Surfaces were rinsed and then immersed in sterile saline. High-frequency ultrasound imaging system was used to scan these surfaces at 55 MHz, and a computer program calculated the average thickness of the biofilm layer from the 3D images. Results 3D pictures of the biofilm layers were obtained. Different cross-sections and plains are easily demonstrated. The average thickness of the 7-day biofilm was significantly bigger than the 2-day on both the membranes and dentinal discs. No structures were observed on the sterile membrane or disc. Conclusion Three-dimensional structural imaging in situ is possible without damaging the biofilm layer in a quick and easy manner and can therefore be used to evaluate biofilms longitudinally as a function of time.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17604896</pmid><doi>10.1016/j.jdent.2007.05.007</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Biofilm Biofilms Biofilms - growth & development Cattle Dentin - diagnostic imaging Dentin - microbiology Dentistry Feasibility Studies Imaging Methods Microbiology NMR Nuclear magnetic resonance Signal processing Streptococcus mutans - physiology Studies Sucrose Time Factors Ultrasonography - methods Ultrasound |
| title | High frequency ultrasound imaging of a single-species biofilm |
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