Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri

Abstract The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional re...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:FEMS microbiology letters 2006-01, Vol.254 (2), p.285-292
Hauptverfasser: Petrella, Stephanie, Renard, Murielle, Ziental-Gelus, Nathalie, Clermont, Dominique, Jarlier, Vincent, Sougakoff, Wladimir
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 292
container_issue 2
container_start_page 285
container_title FEMS microbiology letters
container_volume 254
creator Petrella, Stephanie
Renard, Murielle
Ziental-Gelus, Nathalie
Clermont, Dominique
Jarlier, Vincent
Sougakoff, Wladimir
description Abstract The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.
doi_str_mv 10.1111/j.1574-6968.2005.00028.x
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_70719652</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6968.2005.00028.x</oup_id><sourcerecordid>70719652</sourcerecordid><originalsourceid>FETCH-LOGICAL-o3348-6ecebda220ccb0cfc78b7decd26afa52f03eb07c0f1456080abb7acc43e0f87d3</originalsourceid><addsrcrecordid>eNp1kdtKxDAURYMoznj5BQkIvrWeJs1lwJeheMORedHnkKYp07GdjE2Ll8_yQ_wmM86ooHhecuCsvQl7I4QTiJMwp_M4YSKN-IjLmACwGACIjJ-30PD7sI2GQIWMEhiJAdrzfh6glADfRYOEpykTTA7RXTbTrTadbatX3VVugV2Ju5nFZta6xnnX6BqbWnuPx_j9LaoDqxvtLc5uprgMDM6qrnX5pwd-cD44HaCdUtfeHm7efXR_cX6XXUWT6eV1Np5EjtJURtwamxeaEDAmB1MaIXNRWFMQrkvNSAnU5iAMlEnKOEjQeS60MSm1UEpR0H10svZdtu6xt75TTeWNrWu9sK73SoBIRpyRAB7_Aueubxfhb4pQ4CyVI8YCdbSh-ryxhVq2VaPbF_WVVgDO1sBTVduXnzuoVStqrlbhq1X4atWK-mxFPauL20lYgpyu5a5f_iOO_ojpB_EbkDA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2306548955</pqid></control><display><type>article</type><title>Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Petrella, Stephanie ; Renard, Murielle ; Ziental-Gelus, Nathalie ; Clermont, Dominique ; Jarlier, Vincent ; Sougakoff, Wladimir</creator><creatorcontrib>Petrella, Stephanie ; Renard, Murielle ; Ziental-Gelus, Nathalie ; Clermont, Dominique ; Jarlier, Vincent ; Sougakoff, Wladimir</creatorcontrib><description>Abstract The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2005.00028.x</identifier><identifier>PMID: 16445758</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Substitution ; Anti-Bacterial Agents - pharmacology ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; beta-Lactams - pharmacology ; Chromosomes, Bacterial ; Citrobacter ; Citrobacter amalonaticus ; Citrobacter koseri ; Citrobacter koseri - classification ; Citrobacter koseri - drug effects ; Citrobacter koseri - enzymology ; Citrobacter koseri - genetics ; Clinical isolates ; Humans ; lactamase ; Microbial Sensitivity Tests ; Microbiology ; Missense mutation ; Molecular Sequence Data ; Mutation ; Polymerase chain reaction ; Sequence Analysis, DNA ; Species Specificity ; Transcription</subject><ispartof>FEMS microbiology letters, 2006-01, Vol.254 (2), p.285-292</ispartof><rights>2005 Federation of European Microbiological Societies 2005</rights><rights>2005 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2005.00028.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2005.00028.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16445758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petrella, Stephanie</creatorcontrib><creatorcontrib>Renard, Murielle</creatorcontrib><creatorcontrib>Ziental-Gelus, Nathalie</creatorcontrib><creatorcontrib>Clermont, Dominique</creatorcontrib><creatorcontrib>Jarlier, Vincent</creatorcontrib><creatorcontrib>Sougakoff, Wladimir</creatorcontrib><title>Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.</description><subject>Amino Acid Substitution</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>beta-Lactams - pharmacology</subject><subject>Chromosomes, Bacterial</subject><subject>Citrobacter</subject><subject>Citrobacter amalonaticus</subject><subject>Citrobacter koseri</subject><subject>Citrobacter koseri - classification</subject><subject>Citrobacter koseri - drug effects</subject><subject>Citrobacter koseri - enzymology</subject><subject>Citrobacter koseri - genetics</subject><subject>Clinical isolates</subject><subject>Humans</subject><subject>lactamase</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Missense mutation</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Polymerase chain reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><subject>Transcription</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kdtKxDAURYMoznj5BQkIvrWeJs1lwJeheMORedHnkKYp07GdjE2Ll8_yQ_wmM86ooHhecuCsvQl7I4QTiJMwp_M4YSKN-IjLmACwGACIjJ-30PD7sI2GQIWMEhiJAdrzfh6glADfRYOEpykTTA7RXTbTrTadbatX3VVugV2Ju5nFZta6xnnX6BqbWnuPx_j9LaoDqxvtLc5uprgMDM6qrnX5pwd-cD44HaCdUtfeHm7efXR_cX6XXUWT6eV1Np5EjtJURtwamxeaEDAmB1MaIXNRWFMQrkvNSAnU5iAMlEnKOEjQeS60MSm1UEpR0H10svZdtu6xt75TTeWNrWu9sK73SoBIRpyRAB7_Aueubxfhb4pQ4CyVI8YCdbSh-ryxhVq2VaPbF_WVVgDO1sBTVduXnzuoVStqrlbhq1X4atWK-mxFPauL20lYgpyu5a5f_iOO_ojpB_EbkDA</recordid><startdate>20060101</startdate><enddate>20060101</enddate><creator>Petrella, Stephanie</creator><creator>Renard, Murielle</creator><creator>Ziental-Gelus, Nathalie</creator><creator>Clermont, Dominique</creator><creator>Jarlier, Vincent</creator><creator>Sougakoff, Wladimir</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060101</creationdate><title>Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri</title><author>Petrella, Stephanie ; Renard, Murielle ; Ziental-Gelus, Nathalie ; Clermont, Dominique ; Jarlier, Vincent ; Sougakoff, Wladimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-o3348-6ecebda220ccb0cfc78b7decd26afa52f03eb07c0f1456080abb7acc43e0f87d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Substitution</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>beta-Lactams - pharmacology</topic><topic>Chromosomes, Bacterial</topic><topic>Citrobacter</topic><topic>Citrobacter amalonaticus</topic><topic>Citrobacter koseri</topic><topic>Citrobacter koseri - classification</topic><topic>Citrobacter koseri - drug effects</topic><topic>Citrobacter koseri - enzymology</topic><topic>Citrobacter koseri - genetics</topic><topic>Clinical isolates</topic><topic>Humans</topic><topic>lactamase</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Missense mutation</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Polymerase chain reaction</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petrella, Stephanie</creatorcontrib><creatorcontrib>Renard, Murielle</creatorcontrib><creatorcontrib>Ziental-Gelus, Nathalie</creatorcontrib><creatorcontrib>Clermont, Dominique</creatorcontrib><creatorcontrib>Jarlier, Vincent</creatorcontrib><creatorcontrib>Sougakoff, Wladimir</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petrella, Stephanie</au><au>Renard, Murielle</au><au>Ziental-Gelus, Nathalie</au><au>Clermont, Dominique</au><au>Jarlier, Vincent</au><au>Sougakoff, Wladimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2006-01-01</date><risdate>2006</risdate><volume>254</volume><issue>2</issue><spage>285</spage><epage>292</epage><pages>285-292</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16445758</pmid><doi>10.1111/j.1574-6968.2005.00028.x</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0378-1097
ispartof FEMS microbiology letters, 2006-01, Vol.254 (2), p.285-292
issn 0378-1097
1574-6968
language eng
recordid cdi_proquest_miscellaneous_70719652
source MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current)
subjects Amino Acid Substitution
Anti-Bacterial Agents - pharmacology
beta-Lactamases - genetics
beta-Lactamases - metabolism
beta-Lactams - pharmacology
Chromosomes, Bacterial
Citrobacter
Citrobacter amalonaticus
Citrobacter koseri
Citrobacter koseri - classification
Citrobacter koseri - drug effects
Citrobacter koseri - enzymology
Citrobacter koseri - genetics
Clinical isolates
Humans
lactamase
Microbial Sensitivity Tests
Microbiology
Missense mutation
Molecular Sequence Data
Mutation
Polymerase chain reaction
Sequence Analysis, DNA
Species Specificity
Transcription
title Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T00%3A11%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20the%20chromosomal%20class%20A%20%CE%B2-lactamase%20CKO%20from%20Citrobacter%20koseri&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Petrella,%20Stephanie&rft.date=2006-01-01&rft.volume=254&rft.issue=2&rft.spage=285&rft.epage=292&rft.pages=285-292&rft.issn=0378-1097&rft.eissn=1574-6968&rft_id=info:doi/10.1111/j.1574-6968.2005.00028.x&rft_dat=%3Cproquest_pubme%3E70719652%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2306548955&rft_id=info:pmid/16445758&rft_oup_id=10.1111/j.1574-6968.2005.00028.x&rfr_iscdi=true