Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri
Abstract The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional re...
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Veröffentlicht in: | FEMS microbiology letters 2006-01, Vol.254 (2), p.285-292 |
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creator | Petrella, Stephanie Renard, Murielle Ziental-Gelus, Nathalie Clermont, Dominique Jarlier, Vincent Sougakoff, Wladimir |
description | Abstract
The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp. |
doi_str_mv | 10.1111/j.1574-6968.2005.00028.x |
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The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2005.00028.x</identifier><identifier>PMID: 16445758</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Substitution ; Anti-Bacterial Agents - pharmacology ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; beta-Lactams - pharmacology ; Chromosomes, Bacterial ; Citrobacter ; Citrobacter amalonaticus ; Citrobacter koseri ; Citrobacter koseri - classification ; Citrobacter koseri - drug effects ; Citrobacter koseri - enzymology ; Citrobacter koseri - genetics ; Clinical isolates ; Humans ; lactamase ; Microbial Sensitivity Tests ; Microbiology ; Missense mutation ; Molecular Sequence Data ; Mutation ; Polymerase chain reaction ; Sequence Analysis, DNA ; Species Specificity ; Transcription</subject><ispartof>FEMS microbiology letters, 2006-01, Vol.254 (2), p.285-292</ispartof><rights>2005 Federation of European Microbiological Societies 2005</rights><rights>2005 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2005.00028.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2005.00028.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16445758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petrella, Stephanie</creatorcontrib><creatorcontrib>Renard, Murielle</creatorcontrib><creatorcontrib>Ziental-Gelus, Nathalie</creatorcontrib><creatorcontrib>Clermont, Dominique</creatorcontrib><creatorcontrib>Jarlier, Vincent</creatorcontrib><creatorcontrib>Sougakoff, Wladimir</creatorcontrib><title>Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.</description><subject>Amino Acid Substitution</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>beta-Lactams - pharmacology</subject><subject>Chromosomes, Bacterial</subject><subject>Citrobacter</subject><subject>Citrobacter amalonaticus</subject><subject>Citrobacter koseri</subject><subject>Citrobacter koseri - classification</subject><subject>Citrobacter koseri - drug effects</subject><subject>Citrobacter koseri - enzymology</subject><subject>Citrobacter koseri - genetics</subject><subject>Clinical isolates</subject><subject>Humans</subject><subject>lactamase</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Missense mutation</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Polymerase chain reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><subject>Transcription</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kdtKxDAURYMoznj5BQkIvrWeJs1lwJeheMORedHnkKYp07GdjE2Ll8_yQ_wmM86ooHhecuCsvQl7I4QTiJMwp_M4YSKN-IjLmACwGACIjJ-30PD7sI2GQIWMEhiJAdrzfh6glADfRYOEpykTTA7RXTbTrTadbatX3VVugV2Ju5nFZta6xnnX6BqbWnuPx_j9LaoDqxvtLc5uprgMDM6qrnX5pwd-cD44HaCdUtfeHm7efXR_cX6XXUWT6eV1Np5EjtJURtwamxeaEDAmB1MaIXNRWFMQrkvNSAnU5iAMlEnKOEjQeS60MSm1UEpR0H10svZdtu6xt75TTeWNrWu9sK73SoBIRpyRAB7_Aueubxfhb4pQ4CyVI8YCdbSh-ryxhVq2VaPbF_WVVgDO1sBTVduXnzuoVStqrlbhq1X4atWK-mxFPauL20lYgpyu5a5f_iOO_ojpB_EbkDA</recordid><startdate>20060101</startdate><enddate>20060101</enddate><creator>Petrella, Stephanie</creator><creator>Renard, Murielle</creator><creator>Ziental-Gelus, Nathalie</creator><creator>Clermont, Dominique</creator><creator>Jarlier, Vincent</creator><creator>Sougakoff, Wladimir</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060101</creationdate><title>Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri</title><author>Petrella, Stephanie ; Renard, Murielle ; Ziental-Gelus, Nathalie ; Clermont, Dominique ; Jarlier, Vincent ; Sougakoff, Wladimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-o3348-6ecebda220ccb0cfc78b7decd26afa52f03eb07c0f1456080abb7acc43e0f87d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Substitution</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>beta-Lactams - pharmacology</topic><topic>Chromosomes, Bacterial</topic><topic>Citrobacter</topic><topic>Citrobacter amalonaticus</topic><topic>Citrobacter koseri</topic><topic>Citrobacter koseri - classification</topic><topic>Citrobacter koseri - drug effects</topic><topic>Citrobacter koseri - enzymology</topic><topic>Citrobacter koseri - genetics</topic><topic>Clinical isolates</topic><topic>Humans</topic><topic>lactamase</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Missense mutation</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Polymerase chain reaction</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petrella, Stephanie</creatorcontrib><creatorcontrib>Renard, Murielle</creatorcontrib><creatorcontrib>Ziental-Gelus, Nathalie</creatorcontrib><creatorcontrib>Clermont, Dominique</creatorcontrib><creatorcontrib>Jarlier, Vincent</creatorcontrib><creatorcontrib>Sougakoff, Wladimir</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petrella, Stephanie</au><au>Renard, Murielle</au><au>Ziental-Gelus, Nathalie</au><au>Clermont, Dominique</au><au>Jarlier, Vincent</au><au>Sougakoff, Wladimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2006-01-01</date><risdate>2006</risdate><volume>254</volume><issue>2</issue><spage>285</spage><epage>292</epage><pages>285-292</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
The gene blaCKO encoding the chromosomal class A β-lactamase of Citrobacter koseri was cloned and sequenced. CKO was found to display only 41% identity with SED-1 from Citrobacter sedlakii and 36% with CdiA from Citrobacter amalonaticus (formerly Citrobacter diversus). No transcriptional regulator was found upstream from blaCKO. Silent and missense mutations were detected in four blaCKO genes amplified from different C. koseri clinical isolates, but the CKO variants displayed identical biochemical behaviours. A blaCKO-specific polymerase chain reaction confirmed that blaCKO is present only in C. koseri and therefore represents an interesting tool with which to differentiate C. koseri from the other Citrobacter spp.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16445758</pmid><doi>10.1111/j.1574-6968.2005.00028.x</doi><tpages>8</tpages></addata></record> |
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source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
subjects | Amino Acid Substitution Anti-Bacterial Agents - pharmacology beta-Lactamases - genetics beta-Lactamases - metabolism beta-Lactams - pharmacology Chromosomes, Bacterial Citrobacter Citrobacter amalonaticus Citrobacter koseri Citrobacter koseri - classification Citrobacter koseri - drug effects Citrobacter koseri - enzymology Citrobacter koseri - genetics Clinical isolates Humans lactamase Microbial Sensitivity Tests Microbiology Missense mutation Molecular Sequence Data Mutation Polymerase chain reaction Sequence Analysis, DNA Species Specificity Transcription |
title | Characterization of the chromosomal class A β-lactamase CKO from Citrobacter koseri |
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