Rapid identification of purified enteropathogenic Escherichia coli by microchip electrophoresis
In this work, the potential of PDMS‐based microchip electrophoresis in the identifications and characterizations of microorganism was evaluated. Enteropathogenic E. coli (EPEC) was selected as the model microorganism. In this study, separation parameters such as applied voltage, concentrations of bu...
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| Veröffentlicht in: | Journal of separation science 2007-07, Vol.30 (10), p.1446-1452 |
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| container_title | Journal of separation science |
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| creator | Law, Wai Siang Teng Teng Tay, Elaine Feng, Huatao Yu, Lijun Zhao, Jian Hong Li, Sam Fong Yau |
| description | In this work, the potential of PDMS‐based microchip electrophoresis in the identifications and characterizations of microorganism was evaluated. Enteropathogenic E. coli (EPEC) was selected as the model microorganism. In this study, separation parameters such as applied voltage, concentrations of buffer and buffer modifier, injection voltage, and duration of injection had been investigated and optimized. Determination of EPEC bacteria could be completed within 2 min with good reproducibility. RSDs were less than 0.5 and 5% in migration time and peak area, respectively. Separation efficiency corresponding to plate number of more than 100 000 was achieved. In order to obtain reproducible separations, sample pretreatment was found to be essential. Microchip electrophoresis with LIF detection could potentially revolutionize certain aspects of microbiology involving diagnosis, profiling of pathogens, environmental analysis, and many other areas of study. |
| doi_str_mv | 10.1002/jssc.200600452 |
| format | Article |
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Enteropathogenic E. coli (EPEC) was selected as the model microorganism. In this study, separation parameters such as applied voltage, concentrations of buffer and buffer modifier, injection voltage, and duration of injection had been investigated and optimized. Determination of EPEC bacteria could be completed within 2 min with good reproducibility. RSDs were less than 0.5 and 5% in migration time and peak area, respectively. Separation efficiency corresponding to plate number of more than 100 000 was achieved. In order to obtain reproducible separations, sample pretreatment was found to be essential. Microchip electrophoresis with LIF detection could potentially revolutionize certain aspects of microbiology involving diagnosis, profiling of pathogens, environmental analysis, and many other areas of study.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.200600452</identifier><identifier>PMID: 17623424</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Buffers ; Electrophoresis, Microchip - methods ; Electrophoresis, Microchip - utilization ; Enteropathogenic E. coli ; Escherichia coli O157 - isolation & purification ; Humans ; Laser induced fluorescence ; Microchip electrophoresis ; PDMS</subject><ispartof>Journal of separation science, 2007-07, Vol.30 (10), p.1446-1452</ispartof><rights>Copyright © 2007 WILEY‐VCH Verlag GmbH & Co. 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Microchip electrophoresis with LIF detection could potentially revolutionize certain aspects of microbiology involving diagnosis, profiling of pathogens, environmental analysis, and many other areas of study.</description><subject>Buffers</subject><subject>Electrophoresis, Microchip - methods</subject><subject>Electrophoresis, Microchip - utilization</subject><subject>Enteropathogenic E. coli</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Humans</subject><subject>Laser induced fluorescence</subject><subject>Microchip electrophoresis</subject><subject>PDMS</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPGzEURi1UVCh0yxJ51d2k148ZzyyriKatUEEEhMTG8niuiekkHuyJ2vx7jBKl7FhY14_zfZIPIWcMJgyAf31KyU44QAUgS35AjlnFyqIRTH7Y76E6Ip9SegJgqm7gIzliquJCcnlM9I0ZfEd9h6vRO2_N6MOKBkeHdcxn7Gh-wBgGMy7CI668pRfJLjB6u_CG2tB72m7o0tsY8s1AsUc7Zn4RIiafTsmhM33Cz7t5Qu6-X9xOfxSXV7Of02-XhRVc8UKAMzIvw42wpWBYCcZNVVtXgzQCFTjpat4Cb_Jw0tQWnRGybZuGd3UpTsiXbe8Qw_Ma06iXPlnse7PCsE5agWJSMvEuKLJLWQmVwckWzD9LKaLTQ_RLEzeagX51r1_d6737HDjfNa_bJXb_8Z3sDDRb4K_vcfNOnf41n0_flhfbrE8j_ttnTfyjKyVUqe9_z3QzvxayfpjqB_ECfpuhlw</recordid><startdate>200707</startdate><enddate>200707</enddate><creator>Law, Wai Siang</creator><creator>Teng Teng Tay, Elaine</creator><creator>Feng, Huatao</creator><creator>Yu, Lijun</creator><creator>Zhao, Jian Hong</creator><creator>Li, Sam Fong Yau</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200707</creationdate><title>Rapid identification of purified enteropathogenic Escherichia coli by microchip electrophoresis</title><author>Law, Wai Siang ; Teng Teng Tay, Elaine ; Feng, Huatao ; Yu, Lijun ; Zhao, Jian Hong ; Li, Sam Fong Yau</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3272-30fa40faa2a3c531e6312a68cf804a3e70f4f82b029f82f4a8cefa34bb992d853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Buffers</topic><topic>Electrophoresis, Microchip - methods</topic><topic>Electrophoresis, Microchip - utilization</topic><topic>Enteropathogenic E. coli</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Humans</topic><topic>Laser induced fluorescence</topic><topic>Microchip electrophoresis</topic><topic>PDMS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Law, Wai Siang</creatorcontrib><creatorcontrib>Teng Teng Tay, Elaine</creatorcontrib><creatorcontrib>Feng, Huatao</creatorcontrib><creatorcontrib>Yu, Lijun</creatorcontrib><creatorcontrib>Zhao, Jian Hong</creatorcontrib><creatorcontrib>Li, Sam Fong Yau</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Law, Wai Siang</au><au>Teng Teng Tay, Elaine</au><au>Feng, Huatao</au><au>Yu, Lijun</au><au>Zhao, Jian Hong</au><au>Li, Sam Fong Yau</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid identification of purified enteropathogenic Escherichia coli by microchip electrophoresis</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. 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| language | eng |
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| subjects | Buffers Electrophoresis, Microchip - methods Electrophoresis, Microchip - utilization Enteropathogenic E. coli Escherichia coli O157 - isolation & purification Humans Laser induced fluorescence Microchip electrophoresis PDMS |
| title | Rapid identification of purified enteropathogenic Escherichia coli by microchip electrophoresis |
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