Cytokine profile of human septic shock serum inducing cardiomyocyte contractile dysfunction
This study was designed to measure nitrite/nitrate and cytokine levels of serum obtained from septic shock patients and to describe potential depressant effects of human septic serum on rat cardiomyocytes. Serum was prepared from 10 non-septic patients and 10 patients with documented septic shock. A...
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| Veröffentlicht in: | Physiological research 2007-01, Vol.56 (3), p.291-297 |
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| description | This study was designed to measure nitrite/nitrate and cytokine levels of serum obtained from septic shock patients and to describe potential depressant effects of human septic serum on rat cardiomyocytes. Serum was prepared from 10 non-septic patients and 10 patients with documented septic shock. Adult rat ventricular myocytes were exposed to 20 % serum in the medium. Cardiomyocyte contractility was assessed by measuring shortening fraction and shortening velocity. Serum levels of nitrite/nitrate, a marker of nitric oxide final metabolites, and cytokines (tumor necrosis factor (TNF)-alpha, interleukin (IL) 1beta, 6, 10, 8 and 12p70) were measured. Compared with serum from non-septic patients, serum of septic shock patients induced rapid reduction of the extent and velocity of shortening in isolated cardiomyocytes. Nitrite/nitrate, TNF-alpha, IL-1beta and IL-12p70 concentrations of tested serum for cardiomyocyte studies were not increased in septic serum compared with controls. In contrast, septic serum that induced a depression of in vitro contractility, had increased levels of IL-6, IL-8 and IL-10. We can conclude that the depression of in vitro contractility induced by septic serum is not directly dependent on elevated levels of nitric oxide metabolites, TNF-alpha or IL-1beta. Our results support the view that other cytokines, including IL-6, IL-8 and IL-10, are potent circulating mediators of myocardial depression in cardiomyocytes. |
| doi_str_mv | 10.33549/physiolres.930946 |
| format | Article |
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Serum was prepared from 10 non-septic patients and 10 patients with documented septic shock. Adult rat ventricular myocytes were exposed to 20 % serum in the medium. Cardiomyocyte contractility was assessed by measuring shortening fraction and shortening velocity. Serum levels of nitrite/nitrate, a marker of nitric oxide final metabolites, and cytokines (tumor necrosis factor (TNF)-alpha, interleukin (IL) 1beta, 6, 10, 8 and 12p70) were measured. Compared with serum from non-septic patients, serum of septic shock patients induced rapid reduction of the extent and velocity of shortening in isolated cardiomyocytes. Nitrite/nitrate, TNF-alpha, IL-1beta and IL-12p70 concentrations of tested serum for cardiomyocyte studies were not increased in septic serum compared with controls. In contrast, septic serum that induced a depression of in vitro contractility, had increased levels of IL-6, IL-8 and IL-10. We can conclude that the depression of in vitro contractility induced by septic serum is not directly dependent on elevated levels of nitric oxide metabolites, TNF-alpha or IL-1beta. Our results support the view that other cytokines, including IL-6, IL-8 and IL-10, are potent circulating mediators of myocardial depression in cardiomyocytes.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/physiolres.930946</identifier><identifier>PMID: 16792475</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>Adolescent ; Animals ; Apoptosis ; Cytokines - blood ; Heart surgery ; Humans ; Laboratory animals ; Monoclonal antibodies ; Mortality ; Myocardial Contraction ; Myocytes, Cardiac - metabolism ; Myocytes, Cardiac - physiology ; Nitrates - blood ; Nitrites - blood ; Patients ; Plasma ; Rats ; Rats, Sprague-Dawley ; Rodents ; Shock, Septic - blood ; Shock, Septic - immunology ; Studies</subject><ispartof>Physiological research, 2007-01, Vol.56 (3), p.291-297</ispartof><rights>Copyright Institute of Physiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-d55352efb49cb8f1930ed6f7e7573ec7e5c7129f9e86f84af1c9be013ac2a3a53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16792475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joulin, O</creatorcontrib><creatorcontrib>Petillot, P</creatorcontrib><creatorcontrib>Labalette, M</creatorcontrib><creatorcontrib>Lancel, S</creatorcontrib><creatorcontrib>Neviere, R</creatorcontrib><title>Cytokine profile of human septic shock serum inducing cardiomyocyte contractile dysfunction</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>This study was designed to measure nitrite/nitrate and cytokine levels of serum obtained from septic shock patients and to describe potential depressant effects of human septic serum on rat cardiomyocytes. Serum was prepared from 10 non-septic patients and 10 patients with documented septic shock. Adult rat ventricular myocytes were exposed to 20 % serum in the medium. Cardiomyocyte contractility was assessed by measuring shortening fraction and shortening velocity. Serum levels of nitrite/nitrate, a marker of nitric oxide final metabolites, and cytokines (tumor necrosis factor (TNF)-alpha, interleukin (IL) 1beta, 6, 10, 8 and 12p70) were measured. Compared with serum from non-septic patients, serum of septic shock patients induced rapid reduction of the extent and velocity of shortening in isolated cardiomyocytes. Nitrite/nitrate, TNF-alpha, IL-1beta and IL-12p70 concentrations of tested serum for cardiomyocyte studies were not increased in septic serum compared with controls. In contrast, septic serum that induced a depression of in vitro contractility, had increased levels of IL-6, IL-8 and IL-10. We can conclude that the depression of in vitro contractility induced by septic serum is not directly dependent on elevated levels of nitric oxide metabolites, TNF-alpha or IL-1beta. Our results support the view that other cytokines, including IL-6, IL-8 and IL-10, are potent circulating mediators of myocardial depression in cardiomyocytes.</description><subject>Adolescent</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cytokines - blood</subject><subject>Heart surgery</subject><subject>Humans</subject><subject>Laboratory animals</subject><subject>Monoclonal antibodies</subject><subject>Mortality</subject><subject>Myocardial Contraction</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Myocytes, Cardiac - physiology</subject><subject>Nitrates - blood</subject><subject>Nitrites - blood</subject><subject>Patients</subject><subject>Plasma</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Shock, Septic - blood</subject><subject>Shock, Septic - immunology</subject><subject>Studies</subject><issn>0862-8408</issn><issn>1802-9973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkEtPwzAQhC0EoqXwBzigiAO3FD-SOD6iipdUiQucOESOs6ZuEzvYySH_HkMrVeK0u9I3o51B6JrgJWN5Ju77zRSMaz2EpWBYZMUJmpMS01QIzk7RHJcFTcsMlzN0EcIWY8oxZ-doRgouaMbzOfpcTYPbGQtJ7502LSROJ5uxkzYJ0A9GJWHj1C4efuwSY5tRGfuVKOkb47rJqWmARDk7eKmGX3kzBT3auDt7ic60bANcHeYCfTw9vq9e0vXb8-vqYZ0qxumQNnnOcgq6zoSqS01iFGgKzYHnnIHikCtOqNACykKXmdREiRowYVJRyWTOFuhu7xsjfI8QhqozQUHbSgtuDFUMTUhZ8Aje_gO3bvQ2_lZRQkkhuCgiRPeQ8i4ED7rqvemknyqCq7_eq2Pv1b73KLo5OI91B81Rciia_QBsroPY</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Joulin, O</creator><creator>Petillot, P</creator><creator>Labalette, M</creator><creator>Lancel, S</creator><creator>Neviere, R</creator><general>Institute of Physiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Cytokine profile of human septic shock serum inducing cardiomyocyte contractile dysfunction</title><author>Joulin, O ; Petillot, P ; Labalette, M ; Lancel, S ; Neviere, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-d55352efb49cb8f1930ed6f7e7573ec7e5c7129f9e86f84af1c9be013ac2a3a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adolescent</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cytokines - blood</topic><topic>Heart surgery</topic><topic>Humans</topic><topic>Laboratory animals</topic><topic>Monoclonal antibodies</topic><topic>Mortality</topic><topic>Myocardial Contraction</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Myocytes, Cardiac - physiology</topic><topic>Nitrates - blood</topic><topic>Nitrites - blood</topic><topic>Patients</topic><topic>Plasma</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodents</topic><topic>Shock, Septic - blood</topic><topic>Shock, Septic - immunology</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Joulin, O</creatorcontrib><creatorcontrib>Petillot, P</creatorcontrib><creatorcontrib>Labalette, M</creatorcontrib><creatorcontrib>Lancel, S</creatorcontrib><creatorcontrib>Neviere, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Physiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Joulin, O</au><au>Petillot, P</au><au>Labalette, M</au><au>Lancel, S</au><au>Neviere, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokine profile of human septic shock serum inducing cardiomyocyte contractile dysfunction</atitle><jtitle>Physiological research</jtitle><addtitle>Physiol Res</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>56</volume><issue>3</issue><spage>291</spage><epage>297</epage><pages>291-297</pages><issn>0862-8408</issn><eissn>1802-9973</eissn><abstract>This study was designed to measure nitrite/nitrate and cytokine levels of serum obtained from septic shock patients and to describe potential depressant effects of human septic serum on rat cardiomyocytes. Serum was prepared from 10 non-septic patients and 10 patients with documented septic shock. Adult rat ventricular myocytes were exposed to 20 % serum in the medium. Cardiomyocyte contractility was assessed by measuring shortening fraction and shortening velocity. Serum levels of nitrite/nitrate, a marker of nitric oxide final metabolites, and cytokines (tumor necrosis factor (TNF)-alpha, interleukin (IL) 1beta, 6, 10, 8 and 12p70) were measured. Compared with serum from non-septic patients, serum of septic shock patients induced rapid reduction of the extent and velocity of shortening in isolated cardiomyocytes. Nitrite/nitrate, TNF-alpha, IL-1beta and IL-12p70 concentrations of tested serum for cardiomyocyte studies were not increased in septic serum compared with controls. In contrast, septic serum that induced a depression of in vitro contractility, had increased levels of IL-6, IL-8 and IL-10. We can conclude that the depression of in vitro contractility induced by septic serum is not directly dependent on elevated levels of nitric oxide metabolites, TNF-alpha or IL-1beta. Our results support the view that other cytokines, including IL-6, IL-8 and IL-10, are potent circulating mediators of myocardial depression in cardiomyocytes.</abstract><cop>Czech Republic</cop><pub>Institute of Physiology</pub><pmid>16792475</pmid><doi>10.33549/physiolres.930946</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adolescent Animals Apoptosis Cytokines - blood Heart surgery Humans Laboratory animals Monoclonal antibodies Mortality Myocardial Contraction Myocytes, Cardiac - metabolism Myocytes, Cardiac - physiology Nitrates - blood Nitrites - blood Patients Plasma Rats Rats, Sprague-Dawley Rodents Shock, Septic - blood Shock, Septic - immunology Studies |
| title | Cytokine profile of human septic shock serum inducing cardiomyocyte contractile dysfunction |
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