Anaplasma phagocytophilum infection in a fallow deer (Dama dama) population in a preserve of central Italy
From autumn 2004 to spring 2005, 70 fallow deer (Dama dama), 27 female and 43 male, living in a natural preserve of central Italy were examined by indirect immunofluorescence assay (IFA) to detect specific antibodies to Anaplasma phagocytophilum. Thirty-one (44.28%) sera scored positive: in particul...
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| Veröffentlicht in: | The New microbiologica 2007-04, Vol.30 (2), p.161-165 |
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| creator | Ebani, Valentina Virginia Cerri, Domenico Fratini, Filippo Ampola, Massimiliano Andreani, Ernesto |
| description | From autumn 2004 to spring 2005, 70 fallow deer (Dama dama), 27 female and 43 male, living in a natural preserve of central Italy were examined by indirect immunofluorescence assay (IFA) to detect specific antibodies to Anaplasma phagocytophilum. Thirty-one (44.28%) sera scored positive: in particular 10 fallow deer (8 male and 2 female) scored positive at 40 antibody titer, 21 deer (8 male and 13 female) at > or = 80 titer. EDTA anticoagulated blood samples collected from 29 of the 70 deer examined were tested by a nested-PCR assay to disclose a 546 bp fragment, specific of A. phagocytophilum 16S rRNA gene. Twenty (72.41%) blood samples (8 male and 13 female deer) resulted positive. Fifteen PCR-positive deer also resulted positive to IFA, whereas the remaining six did not show specific antibodies. Three serologically positive animals gave negative results at the nested PCR. Five deer scored negative both to serological tests and PCR. |
| format | Article |
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Thirty-one (44.28%) sera scored positive: in particular 10 fallow deer (8 male and 2 female) scored positive at 40 antibody titer, 21 deer (8 male and 13 female) at > or = 80 titer. EDTA anticoagulated blood samples collected from 29 of the 70 deer examined were tested by a nested-PCR assay to disclose a 546 bp fragment, specific of A. phagocytophilum 16S rRNA gene. Twenty (72.41%) blood samples (8 male and 13 female deer) resulted positive. Fifteen PCR-positive deer also resulted positive to IFA, whereas the remaining six did not show specific antibodies. Three serologically positive animals gave negative results at the nested PCR. Five deer scored negative both to serological tests and PCR.</description><identifier>ISSN: 1121-7138</identifier><identifier>PMID: 17619261</identifier><language>eng</language><publisher>Italy</publisher><subject>Anaplasma ; Anaplasma phagocytophilum - isolation & purification ; Animals ; Antibodies, Bacterial - blood ; Bacteremia - microbiology ; Bacteremia - veterinary ; Dama dama ; Deer ; DNA, Bacterial - analysis ; DNA, Ribosomal - analysis ; DNA, Ribosomal - genetics ; Ehrlichiosis - microbiology ; Ehrlichiosis - veterinary ; Female ; Fluorescent Antibody Technique, Indirect ; Italy ; Male ; Polymerase Chain Reaction - methods ; RNA, Ribosomal, 16S - genetics</subject><ispartof>The New microbiologica, 2007-04, Vol.30 (2), p.161-165</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17619261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ebani, Valentina Virginia</creatorcontrib><creatorcontrib>Cerri, Domenico</creatorcontrib><creatorcontrib>Fratini, Filippo</creatorcontrib><creatorcontrib>Ampola, Massimiliano</creatorcontrib><creatorcontrib>Andreani, Ernesto</creatorcontrib><title>Anaplasma phagocytophilum infection in a fallow deer (Dama dama) population in a preserve of central Italy</title><title>The New microbiologica</title><addtitle>New Microbiol</addtitle><description>From autumn 2004 to spring 2005, 70 fallow deer (Dama dama), 27 female and 43 male, living in a natural preserve of central Italy were examined by indirect immunofluorescence assay (IFA) to detect specific antibodies to Anaplasma phagocytophilum. Thirty-one (44.28%) sera scored positive: in particular 10 fallow deer (8 male and 2 female) scored positive at 40 antibody titer, 21 deer (8 male and 13 female) at > or = 80 titer. EDTA anticoagulated blood samples collected from 29 of the 70 deer examined were tested by a nested-PCR assay to disclose a 546 bp fragment, specific of A. phagocytophilum 16S rRNA gene. Twenty (72.41%) blood samples (8 male and 13 female deer) resulted positive. Fifteen PCR-positive deer also resulted positive to IFA, whereas the remaining six did not show specific antibodies. Three serologically positive animals gave negative results at the nested PCR. Five deer scored negative both to serological tests and PCR.</description><subject>Anaplasma</subject><subject>Anaplasma phagocytophilum - isolation & purification</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bacteremia - microbiology</subject><subject>Bacteremia - veterinary</subject><subject>Dama dama</subject><subject>Deer</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Ribosomal - analysis</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ehrlichiosis - microbiology</subject><subject>Ehrlichiosis - veterinary</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Italy</subject><subject>Male</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA, Ribosomal, 16S - genetics</subject><issn>1121-7138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhj2AaFX6F5AnBEMkf8R2Olblo5UqscAcXRybpnJiYyeg_nssUcTIDe_d8Nyr9-4CzSlltFCUVzO0TOlIcklB2YpdoRlVkq6YpHN0XA8QHKQecDjAu9en0YdD56Yed4M1euz8kCcM2IJz_gu3xkR89wB5oc1yj4MPk4M_LkSTTPw02FuszTBGcHg3gjtdo8vskczy3Bfo7enxdbMt9i_Pu816XwRWkrGoFJEN41wRyyxXlZaSNo1sFRADtBQCViVtBVCrtCyV4Fa0FZfA88Fc6YYv0O2Pb4j-YzJprPsuaeMcDMZPqVZEUUJK8i_ISMlFDpLBmzM4Nb1p6xC7HuKp_n0j_wZeBm2Q</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Ebani, Valentina Virginia</creator><creator>Cerri, Domenico</creator><creator>Fratini, Filippo</creator><creator>Ampola, Massimiliano</creator><creator>Andreani, Ernesto</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200704</creationdate><title>Anaplasma phagocytophilum infection in a fallow deer (Dama dama) population in a preserve of central Italy</title><author>Ebani, Valentina Virginia ; Cerri, Domenico ; Fratini, Filippo ; Ampola, Massimiliano ; Andreani, Ernesto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p240t-8706b23370f2f378c661bb6d7a0ea1455a941d5a1f7c64753f5d836a311237cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Anaplasma</topic><topic>Anaplasma phagocytophilum - isolation & purification</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Bacteremia - microbiology</topic><topic>Bacteremia - veterinary</topic><topic>Dama dama</topic><topic>Deer</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Ribosomal - analysis</topic><topic>DNA, Ribosomal - genetics</topic><topic>Ehrlichiosis - microbiology</topic><topic>Ehrlichiosis - veterinary</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Italy</topic><topic>Male</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA, Ribosomal, 16S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebani, Valentina Virginia</creatorcontrib><creatorcontrib>Cerri, Domenico</creatorcontrib><creatorcontrib>Fratini, Filippo</creatorcontrib><creatorcontrib>Ampola, Massimiliano</creatorcontrib><creatorcontrib>Andreani, Ernesto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The New microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ebani, Valentina Virginia</au><au>Cerri, Domenico</au><au>Fratini, Filippo</au><au>Ampola, Massimiliano</au><au>Andreani, Ernesto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anaplasma phagocytophilum infection in a fallow deer (Dama dama) population in a preserve of central Italy</atitle><jtitle>The New microbiologica</jtitle><addtitle>New Microbiol</addtitle><date>2007-04</date><risdate>2007</risdate><volume>30</volume><issue>2</issue><spage>161</spage><epage>165</epage><pages>161-165</pages><issn>1121-7138</issn><abstract>From autumn 2004 to spring 2005, 70 fallow deer (Dama dama), 27 female and 43 male, living in a natural preserve of central Italy were examined by indirect immunofluorescence assay (IFA) to detect specific antibodies to Anaplasma phagocytophilum. Thirty-one (44.28%) sera scored positive: in particular 10 fallow deer (8 male and 2 female) scored positive at 40 antibody titer, 21 deer (8 male and 13 female) at > or = 80 titer. EDTA anticoagulated blood samples collected from 29 of the 70 deer examined were tested by a nested-PCR assay to disclose a 546 bp fragment, specific of A. phagocytophilum 16S rRNA gene. Twenty (72.41%) blood samples (8 male and 13 female deer) resulted positive. Fifteen PCR-positive deer also resulted positive to IFA, whereas the remaining six did not show specific antibodies. Three serologically positive animals gave negative results at the nested PCR. Five deer scored negative both to serological tests and PCR.</abstract><cop>Italy</cop><pmid>17619261</pmid><tpages>5</tpages></addata></record> |
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| subjects | Anaplasma Anaplasma phagocytophilum - isolation & purification Animals Antibodies, Bacterial - blood Bacteremia - microbiology Bacteremia - veterinary Dama dama Deer DNA, Bacterial - analysis DNA, Ribosomal - analysis DNA, Ribosomal - genetics Ehrlichiosis - microbiology Ehrlichiosis - veterinary Female Fluorescent Antibody Technique, Indirect Italy Male Polymerase Chain Reaction - methods RNA, Ribosomal, 16S - genetics |
| title | Anaplasma phagocytophilum infection in a fallow deer (Dama dama) population in a preserve of central Italy |
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