Regional differences in expression of specific markers for human embryonic stem cells
Abstract Characterization of human embryonic stem cell (hESC) lines derived from the inner cell masses of blastocysts generally includes expression analysis of markers such as OCT4, NANOG, SSEA3, SSEA4, TRA-1–60 and TRA-1–81. Expression is usually detected by immunocytochemical staining of entire co...
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| Veröffentlicht in: | Reproductive biomedicine online 2007, Vol.15 (1), p.89-98 |
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| creator | Laursen, Steen B Møllgård, Kjeld Olesen, Christian Oliveri, Roberto S Brøchner, Christian B Byskov, Anne Grete Andersen, Anders Nyboe Høyer, Poul Erik Tommerup, Niels Andersen, Claus Yding |
| description | Abstract Characterization of human embryonic stem cell (hESC) lines derived from the inner cell masses of blastocysts generally includes expression analysis of markers such as OCT4, NANOG, SSEA3, SSEA4, TRA-1–60 and TRA-1–81. Expression is usually detected by immunocytochemical staining of entire colonies of hESC, using one colony for each individual marker. Four newly established hESC lines showed the expected expression pattern and were capable of differentiating into the three germ layers in vitro . Neighbouring sections of entire colonies grown for 4, 11, 21 and 28 days respectively were stained with different markers to study the regional distribution and cellular co-expression. TRA-1–60 staining defined the hESC territory at all time points analysed. This territory comprised a characteristic OCT4 and NANOG staining often in overlapping sub-regions. Staining intensity of nuclei varied from strong OCT4 staining to weak or absent NANOG staining, and vice versa. SSEA4 staining was only observed in small clusters or single cells and not confined to the TRA territory. Co-expression of all markers was only detected in small areas. SSEA1 expression was found exclusively outside the TRA territory. In conclusion, pronounced regional differences in the expression of markers considered specific for undifferentiated hESC may suggest the existence of different cell populations. |
| doi_str_mv | 10.1016/S1472-6483(10)60697-9 |
| format | Article |
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Expression is usually detected by immunocytochemical staining of entire colonies of hESC, using one colony for each individual marker. Four newly established hESC lines showed the expected expression pattern and were capable of differentiating into the three germ layers in vitro . Neighbouring sections of entire colonies grown for 4, 11, 21 and 28 days respectively were stained with different markers to study the regional distribution and cellular co-expression. TRA-1–60 staining defined the hESC territory at all time points analysed. This territory comprised a characteristic OCT4 and NANOG staining often in overlapping sub-regions. Staining intensity of nuclei varied from strong OCT4 staining to weak or absent NANOG staining, and vice versa. SSEA4 staining was only observed in small clusters or single cells and not confined to the TRA territory. Co-expression of all markers was only detected in small areas. SSEA1 expression was found exclusively outside the TRA territory. In conclusion, pronounced regional differences in the expression of markers considered specific for undifferentiated hESC may suggest the existence of different cell populations.</description><identifier>ISSN: 1472-6483</identifier><identifier>EISSN: 1472-6491</identifier><identifier>DOI: 10.1016/S1472-6483(10)60697-9</identifier><identifier>PMID: 17623545</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Antigens, Surface - genetics ; Antigens, Surface - metabolism ; Antigens, Tumor-Associated, Carbohydrate - genetics ; Antigens, Tumor-Associated, Carbohydrate - metabolism ; Biomarkers - metabolism ; Cell Line ; Cell Lineage ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - physiology ; Glycosphingolipids - genetics ; Glycosphingolipids - metabolism ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; human embryonic stem cells ; Humans ; Immunohistochemistry ; Lewis X Antigen - genetics ; Lewis X Antigen - metabolism ; NANOG ; Nanog Homeobox Protein ; Obstetrics and Gynecology ; OCT4 ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; pluripotency marker ; Proteoglycans - genetics ; Proteoglycans - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; SSEA4 ; Stage-Specific Embryonic Antigens ; TRA-1–60</subject><ispartof>Reproductive biomedicine online, 2007, Vol.15 (1), p.89-98</ispartof><rights>Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK</rights><rights>2007 Reproductive Healthcare Ltd, Duck End Farm, Dry Drayton, Cambridge CB23 8DB, UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-e3d0a1b496a40348e9df3d94e03bac77a158b5660d9b25d0367dbcc4d4b2e50f3</citedby><cites>FETCH-LOGICAL-c465t-e3d0a1b496a40348e9df3d94e03bac77a158b5660d9b25d0367dbcc4d4b2e50f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1472-6483(10)60697-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27906,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17623545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laursen, Steen B</creatorcontrib><creatorcontrib>Møllgård, Kjeld</creatorcontrib><creatorcontrib>Olesen, Christian</creatorcontrib><creatorcontrib>Oliveri, Roberto S</creatorcontrib><creatorcontrib>Brøchner, Christian B</creatorcontrib><creatorcontrib>Byskov, Anne Grete</creatorcontrib><creatorcontrib>Andersen, Anders Nyboe</creatorcontrib><creatorcontrib>Høyer, Poul Erik</creatorcontrib><creatorcontrib>Tommerup, Niels</creatorcontrib><creatorcontrib>Andersen, Claus Yding</creatorcontrib><title>Regional differences in expression of specific markers for human embryonic stem cells</title><title>Reproductive biomedicine online</title><addtitle>Reprod Biomed Online</addtitle><description>Abstract Characterization of human embryonic stem cell (hESC) lines derived from the inner cell masses of blastocysts generally includes expression analysis of markers such as OCT4, NANOG, SSEA3, SSEA4, TRA-1–60 and TRA-1–81. Expression is usually detected by immunocytochemical staining of entire colonies of hESC, using one colony for each individual marker. Four newly established hESC lines showed the expected expression pattern and were capable of differentiating into the three germ layers in vitro . Neighbouring sections of entire colonies grown for 4, 11, 21 and 28 days respectively were stained with different markers to study the regional distribution and cellular co-expression. TRA-1–60 staining defined the hESC territory at all time points analysed. This territory comprised a characteristic OCT4 and NANOG staining often in overlapping sub-regions. Staining intensity of nuclei varied from strong OCT4 staining to weak or absent NANOG staining, and vice versa. SSEA4 staining was only observed in small clusters or single cells and not confined to the TRA territory. Co-expression of all markers was only detected in small areas. SSEA1 expression was found exclusively outside the TRA territory. In conclusion, pronounced regional differences in the expression of markers considered specific for undifferentiated hESC may suggest the existence of different cell populations.</description><subject>Antigens, Surface - genetics</subject><subject>Antigens, Surface - metabolism</subject><subject>Antigens, Tumor-Associated, Carbohydrate - genetics</subject><subject>Antigens, Tumor-Associated, Carbohydrate - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Cell Line</subject><subject>Cell Lineage</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Glycosphingolipids - genetics</subject><subject>Glycosphingolipids - metabolism</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>human embryonic stem cells</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Lewis X Antigen - genetics</subject><subject>Lewis X Antigen - metabolism</subject><subject>NANOG</subject><subject>Nanog Homeobox Protein</subject><subject>Obstetrics and Gynecology</subject><subject>OCT4</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>pluripotency marker</subject><subject>Proteoglycans - genetics</subject><subject>Proteoglycans - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>SSEA4</subject><subject>Stage-Specific Embryonic Antigens</subject><subject>TRA-1–60</subject><issn>1472-6483</issn><issn>1472-6491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EoqXwE0A5ITgExrFjxxcQqviSKlUCerYcewJuk3jxbCr239fZXYHUC6exZt55x_MMY885vOHA1dvvXOqmVrITrzi8VqCMrs0DdnpMG_7w77sTJ-wJ0TUA76ATj9kJ16oRrWxP2dU3_BnT7MYqxGHAjLNHquJc4Z9NRqJSq9JQ0QZ9HKKvJpdvMFM1pFz9WiZXhFOfd2kuNdriVHkcR3rKHg1uJHx2jGfs6tPHH-df6ovLz1_PP1zUXqp2W6MI4HgvjXIShOzQhEEEIxFE77zWjrdd3yoFwfRNG0AoHXrvZZB9gy0M4oy9PPhucvq9IG3tFGn9gZsxLWQ1aJCguyJsD0KfE1HGwW5yLLvsLAe78rR7nnaFtab2PK0pfS-OA5Z-wvCv6wiwCN4fBFjWvI2YLfm4Qgwxo9_akOJ_R7y75-DHWHC68QZ3SNdpyeU8ZLmlxsLBZPXgsHcw4g6K45ro</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Laursen, Steen B</creator><creator>Møllgård, Kjeld</creator><creator>Olesen, Christian</creator><creator>Oliveri, Roberto S</creator><creator>Brøchner, Christian B</creator><creator>Byskov, Anne Grete</creator><creator>Andersen, Anders Nyboe</creator><creator>Høyer, Poul Erik</creator><creator>Tommerup, Niels</creator><creator>Andersen, Claus Yding</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Regional differences in expression of specific markers for human embryonic stem cells</title><author>Laursen, Steen B ; Møllgård, Kjeld ; Olesen, Christian ; Oliveri, Roberto S ; Brøchner, Christian B ; Byskov, Anne Grete ; Andersen, Anders Nyboe ; Høyer, Poul Erik ; Tommerup, Niels ; Andersen, Claus Yding</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-e3d0a1b496a40348e9df3d94e03bac77a158b5660d9b25d0367dbcc4d4b2e50f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Antigens, Surface - genetics</topic><topic>Antigens, Surface - metabolism</topic><topic>Antigens, Tumor-Associated, Carbohydrate - genetics</topic><topic>Antigens, Tumor-Associated, Carbohydrate - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Cell Line</topic><topic>Cell Lineage</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Glycosphingolipids - genetics</topic><topic>Glycosphingolipids - metabolism</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>human embryonic stem cells</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Lewis X Antigen - genetics</topic><topic>Lewis X Antigen - metabolism</topic><topic>NANOG</topic><topic>Nanog Homeobox Protein</topic><topic>Obstetrics and Gynecology</topic><topic>OCT4</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Octamer Transcription Factor-3 - metabolism</topic><topic>pluripotency marker</topic><topic>Proteoglycans - genetics</topic><topic>Proteoglycans - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>SSEA4</topic><topic>Stage-Specific Embryonic Antigens</topic><topic>TRA-1–60</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laursen, Steen B</creatorcontrib><creatorcontrib>Møllgård, Kjeld</creatorcontrib><creatorcontrib>Olesen, Christian</creatorcontrib><creatorcontrib>Oliveri, Roberto S</creatorcontrib><creatorcontrib>Brøchner, Christian B</creatorcontrib><creatorcontrib>Byskov, Anne Grete</creatorcontrib><creatorcontrib>Andersen, Anders Nyboe</creatorcontrib><creatorcontrib>Høyer, Poul Erik</creatorcontrib><creatorcontrib>Tommerup, Niels</creatorcontrib><creatorcontrib>Andersen, Claus Yding</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive biomedicine online</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laursen, Steen B</au><au>Møllgård, Kjeld</au><au>Olesen, Christian</au><au>Oliveri, Roberto S</au><au>Brøchner, Christian B</au><au>Byskov, Anne Grete</au><au>Andersen, Anders Nyboe</au><au>Høyer, Poul Erik</au><au>Tommerup, Niels</au><au>Andersen, Claus Yding</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regional differences in expression of specific markers for human embryonic stem cells</atitle><jtitle>Reproductive biomedicine online</jtitle><addtitle>Reprod Biomed Online</addtitle><date>2007</date><risdate>2007</risdate><volume>15</volume><issue>1</issue><spage>89</spage><epage>98</epage><pages>89-98</pages><issn>1472-6483</issn><eissn>1472-6491</eissn><abstract>Abstract Characterization of human embryonic stem cell (hESC) lines derived from the inner cell masses of blastocysts generally includes expression analysis of markers such as OCT4, NANOG, SSEA3, SSEA4, TRA-1–60 and TRA-1–81. Expression is usually detected by immunocytochemical staining of entire colonies of hESC, using one colony for each individual marker. Four newly established hESC lines showed the expected expression pattern and were capable of differentiating into the three germ layers in vitro . Neighbouring sections of entire colonies grown for 4, 11, 21 and 28 days respectively were stained with different markers to study the regional distribution and cellular co-expression. TRA-1–60 staining defined the hESC territory at all time points analysed. This territory comprised a characteristic OCT4 and NANOG staining often in overlapping sub-regions. Staining intensity of nuclei varied from strong OCT4 staining to weak or absent NANOG staining, and vice versa. SSEA4 staining was only observed in small clusters or single cells and not confined to the TRA territory. Co-expression of all markers was only detected in small areas. SSEA1 expression was found exclusively outside the TRA territory. In conclusion, pronounced regional differences in the expression of markers considered specific for undifferentiated hESC may suggest the existence of different cell populations.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>17623545</pmid><doi>10.1016/S1472-6483(10)60697-9</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens, Surface - genetics Antigens, Surface - metabolism Antigens, Tumor-Associated, Carbohydrate - genetics Antigens, Tumor-Associated, Carbohydrate - metabolism Biomarkers - metabolism Cell Line Cell Lineage DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Embryonic Stem Cells - cytology Embryonic Stem Cells - physiology Glycosphingolipids - genetics Glycosphingolipids - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism human embryonic stem cells Humans Immunohistochemistry Lewis X Antigen - genetics Lewis X Antigen - metabolism NANOG Nanog Homeobox Protein Obstetrics and Gynecology OCT4 Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism pluripotency marker Proteoglycans - genetics Proteoglycans - metabolism Reverse Transcriptase Polymerase Chain Reaction SSEA4 Stage-Specific Embryonic Antigens TRA-1–60 |
| title | Regional differences in expression of specific markers for human embryonic stem cells |
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