Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium
Proof-in-concept biosensors were prepared for the rapid detection of Salmonella typhimurium in solution, based on affinity-selected filamentous phage prepared as probes physically adsorbed to piezoelectric transducers. Quantitative deposition studies indicated that ≈3 × 10 10 phage particles/cm 2 co...
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| Veröffentlicht in: | Biosensors & bioelectronics 2006-02, Vol.21 (8), p.1434-1442 |
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| container_title | Biosensors & bioelectronics |
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| creator | Olsen, Eric V. Sorokulova, Iryna B. Petrenko, Valery A. Chen, I-Hsuan Barbaree, James M. Vodyanoy, Vitaly J. |
| description | Proof-in-concept biosensors were prepared for the rapid detection of
Salmonella typhimurium in solution, based on affinity-selected filamentous phage prepared as probes physically adsorbed to piezoelectric transducers. Quantitative deposition studies indicated that ≈3
×
10
10
phage particles/cm
2 could be irreversibly adsorbed for 1
h at room temperature to prepare working biosensors. The quality of phage deposition was monitored by fluorescent microscopy. Specific-bacterial binding resulted in resonance frequency changes of prepared sensors, which were evaluated using linear regression analysis. Sensors possessed a rapid response time of |
| doi_str_mv | 10.1016/j.bios.2005.06.004 |
| format | Article |
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Salmonella typhimurium in solution, based on affinity-selected filamentous phage prepared as probes physically adsorbed to piezoelectric transducers. Quantitative deposition studies indicated that ≈3
×
10
10
phage particles/cm
2 could be irreversibly adsorbed for 1
h at room temperature to prepare working biosensors. The quality of phage deposition was monitored by fluorescent microscopy. Specific-bacterial binding resulted in resonance frequency changes of prepared sensors, which were evaluated using linear regression analysis. Sensors possessed a rapid response time of <180
s, had a low-detection limit of 10
2
cells/ml and were linear over a range of 10
1–10
7
cells/ml with a sensitivity of 10.9
Hz per order of magnitude of
S. typhimurium concentration. Viscosity effects due to increasing bacterial concentration and non-specific binding were not significant to the piezoelectric platform as confirmed by dose–response analysis. Phage–bacterial binding was confirmed by fluorescence and scanning electron microscopy. Overall, phage may constitute effective bioreceptors for use with analytical platforms for detecting and monitoring bacterial agents, including use in food products and possibly biological warfare applications.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2005.06.004</identifier><identifier>PMID: 16085408</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Bacteria ; Bacteriophages - isolation & purification ; Bacteriophages - physiology ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensor ; Biosensors ; Biotechnology ; Colony Count, Microbial - instrumentation ; Colony Count, Microbial - methods ; Electrochemistry - instrumentation ; Electrochemistry - methods ; Equipment Design ; Equipment Failure Analysis ; Filamentous bacteriophage ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Molecular Probe Techniques - instrumentation ; Phage display ; Piezoelectric ; Quartz crystal microbalance ; Reproducibility of Results ; Salmonella typhimurium ; Salmonella typhimurium - isolation & purification ; Salmonella typhimurium - virology ; Sensitivity and Specificity ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2006-02, Vol.21 (8), p.1434-1442</ispartof><rights>2005</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-91e5ea64be9dee5520c350eff72ed1ab5e9591ca906a14aa4bbbb3fdaabb310c3</citedby><cites>FETCH-LOGICAL-c415t-91e5ea64be9dee5520c350eff72ed1ab5e9591ca906a14aa4bbbb3fdaabb310c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566305001831$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17491381$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16085408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olsen, Eric V.</creatorcontrib><creatorcontrib>Sorokulova, Iryna B.</creatorcontrib><creatorcontrib>Petrenko, Valery A.</creatorcontrib><creatorcontrib>Chen, I-Hsuan</creatorcontrib><creatorcontrib>Barbaree, James M.</creatorcontrib><creatorcontrib>Vodyanoy, Vitaly J.</creatorcontrib><title>Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Proof-in-concept biosensors were prepared for the rapid detection of
Salmonella typhimurium in solution, based on affinity-selected filamentous phage prepared as probes physically adsorbed to piezoelectric transducers. Quantitative deposition studies indicated that ≈3
×
10
10
phage particles/cm
2 could be irreversibly adsorbed for 1
h at room temperature to prepare working biosensors. The quality of phage deposition was monitored by fluorescent microscopy. Specific-bacterial binding resulted in resonance frequency changes of prepared sensors, which were evaluated using linear regression analysis. Sensors possessed a rapid response time of <180
s, had a low-detection limit of 10
2
cells/ml and were linear over a range of 10
1–10
7
cells/ml with a sensitivity of 10.9
Hz per order of magnitude of
S. typhimurium concentration. Viscosity effects due to increasing bacterial concentration and non-specific binding were not significant to the piezoelectric platform as confirmed by dose–response analysis. Phage–bacterial binding was confirmed by fluorescence and scanning electron microscopy. Overall, phage may constitute effective bioreceptors for use with analytical platforms for detecting and monitoring bacterial agents, including use in food products and possibly biological warfare applications.</description><subject>Bacteria</subject><subject>Bacteriophages - isolation & purification</subject><subject>Bacteriophages - physiology</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Colony Count, Microbial - instrumentation</subject><subject>Colony Count, Microbial - methods</subject><subject>Electrochemistry - instrumentation</subject><subject>Electrochemistry - methods</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Filamentous bacteriophage</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular Probe Techniques - instrumentation</subject><subject>Phage display</subject><subject>Piezoelectric</subject><subject>Quartz crystal microbalance</subject><subject>Reproducibility of Results</subject><subject>Salmonella typhimurium</subject><subject>Salmonella typhimurium - isolation & purification</subject><subject>Salmonella typhimurium - virology</subject><subject>Sensitivity and Specificity</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAUxEVJabZpv0APQZf0ZvfJtuQV5BJC-gcCPbQ9i2f5qdFiWxvJTthvX5ldyC3V5YH4zTDMMPZJQClAqC-7svMhlRWALEGVAM0bthHbti6aqpZnbANaqkIqVZ-z9yntAKAVGt6xc6FgKxvYbli4cc5Pfj4UiQayM_Xc-QFHmuawJN5h_oo-7B_wL3FMHPk-ho64C5GjzcjsLX_GJ-I5S09ztggx8eD4LxzGMNEwIJ8P-wc_LtEv4wf21uGQ6OPpXrA_X-9-334v7n9--3F7c1_YRsi50IIkoWo60j2RlBXYWgI511bUC-wkaamFRQ0KRYPYdPnVrkfMR2T4gn0--ua4jwul2Yw-2TXNRDm1aaGFWin9X7BaW2tbmcHqCNoYUorkzD76EePBCDDrHmZn1j1WhTSgTN4jiy5P7ks3Uv8iOQ2QgasTgMni4CJO1qcXrm20qLcic9dHjnJpT56iSdbTZKn3MXdu-uBfy_EPiyeseg</recordid><startdate>20060215</startdate><enddate>20060215</enddate><creator>Olsen, Eric V.</creator><creator>Sorokulova, Iryna B.</creator><creator>Petrenko, Valery A.</creator><creator>Chen, I-Hsuan</creator><creator>Barbaree, James M.</creator><creator>Vodyanoy, Vitaly J.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060215</creationdate><title>Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium</title><author>Olsen, Eric V. ; Sorokulova, Iryna B. ; Petrenko, Valery A. ; Chen, I-Hsuan ; Barbaree, James M. ; Vodyanoy, Vitaly J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-91e5ea64be9dee5520c350eff72ed1ab5e9591ca906a14aa4bbbb3fdaabb310c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacteria</topic><topic>Bacteriophages - isolation & purification</topic><topic>Bacteriophages - physiology</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Colony Count, Microbial - instrumentation</topic><topic>Colony Count, Microbial - methods</topic><topic>Electrochemistry - instrumentation</topic><topic>Electrochemistry - methods</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Filamentous bacteriophage</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular Probe Techniques - instrumentation</topic><topic>Phage display</topic><topic>Piezoelectric</topic><topic>Quartz crystal microbalance</topic><topic>Reproducibility of Results</topic><topic>Salmonella typhimurium</topic><topic>Salmonella typhimurium - isolation & purification</topic><topic>Salmonella typhimurium - virology</topic><topic>Sensitivity and Specificity</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olsen, Eric V.</creatorcontrib><creatorcontrib>Sorokulova, Iryna B.</creatorcontrib><creatorcontrib>Petrenko, Valery A.</creatorcontrib><creatorcontrib>Chen, I-Hsuan</creatorcontrib><creatorcontrib>Barbaree, James M.</creatorcontrib><creatorcontrib>Vodyanoy, Vitaly J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olsen, Eric V.</au><au>Sorokulova, Iryna B.</au><au>Petrenko, Valery A.</au><au>Chen, I-Hsuan</au><au>Barbaree, James M.</au><au>Vodyanoy, Vitaly J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2006-02-15</date><risdate>2006</risdate><volume>21</volume><issue>8</issue><spage>1434</spage><epage>1442</epage><pages>1434-1442</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Proof-in-concept biosensors were prepared for the rapid detection of
Salmonella typhimurium in solution, based on affinity-selected filamentous phage prepared as probes physically adsorbed to piezoelectric transducers. Quantitative deposition studies indicated that ≈3
×
10
10
phage particles/cm
2 could be irreversibly adsorbed for 1
h at room temperature to prepare working biosensors. The quality of phage deposition was monitored by fluorescent microscopy. Specific-bacterial binding resulted in resonance frequency changes of prepared sensors, which were evaluated using linear regression analysis. Sensors possessed a rapid response time of <180
s, had a low-detection limit of 10
2
cells/ml and were linear over a range of 10
1–10
7
cells/ml with a sensitivity of 10.9
Hz per order of magnitude of
S. typhimurium concentration. Viscosity effects due to increasing bacterial concentration and non-specific binding were not significant to the piezoelectric platform as confirmed by dose–response analysis. Phage–bacterial binding was confirmed by fluorescence and scanning electron microscopy. Overall, phage may constitute effective bioreceptors for use with analytical platforms for detecting and monitoring bacterial agents, including use in food products and possibly biological warfare applications.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>16085408</pmid><doi>10.1016/j.bios.2005.06.004</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biosensors & bioelectronics, 2006-02, Vol.21 (8), p.1434-1442 |
| issn | 0956-5663 1873-4235 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacteria Bacteriophages - isolation & purification Bacteriophages - physiology Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensor Biosensors Biotechnology Colony Count, Microbial - instrumentation Colony Count, Microbial - methods Electrochemistry - instrumentation Electrochemistry - methods Equipment Design Equipment Failure Analysis Filamentous bacteriophage Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Molecular Probe Techniques - instrumentation Phage display Piezoelectric Quartz crystal microbalance Reproducibility of Results Salmonella typhimurium Salmonella typhimurium - isolation & purification Salmonella typhimurium - virology Sensitivity and Specificity Various methods and equipments |
| title | Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium |
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