DNA sequence analysis of rdxA and frxA from paired metronidazole-sensitive and -resistant Helicobacter pylori isolates obtained from patients with heteroresistance
Genomic variations of rdxA and frxA genes in eight pairs of metronidazole (MTZ)-sensitive and -resistant isolates of Helicobacter pylori were investigated. The paired strains from each single biopsy had identical lspA- glmM restriction fragment length polymorphism profiles, and the differences in MT...
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| Veröffentlicht in: | International journal of antimicrobial agents 2006-02, Vol.27 (2), p.152-158 |
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| container_title | International journal of antimicrobial agents |
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| creator | Matteo, Mario José Pérez, Cecilia Valeria Domingo, Mara Roxana Olmos, Martín Sanchez, Cristian Catalano, Mariana |
| description | Genomic variations of
rdxA and
frxA genes in eight pairs of metronidazole (MTZ)-sensitive and -resistant isolates of
Helicobacter pylori were investigated. The paired strains from each single biopsy had identical
lspA-
glmM restriction fragment length polymorphism profiles, and the differences in MTZ susceptibility were mostly accounted for by mutations in the
rdxA gene. Truncation of RdxA is associated with a minimum inhibitory concentration of 64–128
mg/L. Early truncation of FrxA was observed both in susceptible and resistant isolates of seven paired strains. Inactivation of
frxA might play a significant role only in one low-level MTZ-resistant isolate where a missense mutation was found in the
rdxA gene. |
| doi_str_mv | 10.1016/j.ijantimicag.2005.09.019 |
| format | Article |
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rdxA and
frxA genes in eight pairs of metronidazole (MTZ)-sensitive and -resistant isolates of
Helicobacter pylori were investigated. The paired strains from each single biopsy had identical
lspA-
glmM restriction fragment length polymorphism profiles, and the differences in MTZ susceptibility were mostly accounted for by mutations in the
rdxA gene. Truncation of RdxA is associated with a minimum inhibitory concentration of 64–128
mg/L. Early truncation of FrxA was observed both in susceptible and resistant isolates of seven paired strains. Inactivation of
frxA might play a significant role only in one low-level MTZ-resistant isolate where a missense mutation was found in the
rdxA gene.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2005.09.019</identifier><identifier>PMID: 16426819</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Anti-Bacterial Agents - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacterial Proteins - genetics ; Biological and medical sciences ; DNA, Bacterial - genetics ; Drug Resistance, Bacterial - genetics ; frxA ; Genes, Bacterial ; Helicobacter Infections - drug therapy ; Helicobacter Infections - microbiology ; Helicobacter pylori ; Helicobacter pylori - drug effects ; Helicobacter pylori - genetics ; Helicobacter pylori - isolation & purification ; Humans ; Medical sciences ; Metronidazole - pharmacology ; Metronidazole resistance ; Molecular Sequence Data ; Mutation ; Nitroreductases - genetics ; Pharmacology. Drug treatments ; Polymorphism, Restriction Fragment Length ; rdxA ; Sequence Homology, Amino Acid</subject><ispartof>International journal of antimicrobial agents, 2006-02, Vol.27 (2), p.152-158</ispartof><rights>2005 Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-9b052ba721dd571b238cc704a274316511f3998965c69f378dd197d4679c17933</citedby><cites>FETCH-LOGICAL-c405t-9b052ba721dd571b238cc704a274316511f3998965c69f378dd197d4679c17933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijantimicag.2005.09.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17459831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16426819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matteo, Mario José</creatorcontrib><creatorcontrib>Pérez, Cecilia Valeria</creatorcontrib><creatorcontrib>Domingo, Mara Roxana</creatorcontrib><creatorcontrib>Olmos, Martín</creatorcontrib><creatorcontrib>Sanchez, Cristian</creatorcontrib><creatorcontrib>Catalano, Mariana</creatorcontrib><title>DNA sequence analysis of rdxA and frxA from paired metronidazole-sensitive and -resistant Helicobacter pylori isolates obtained from patients with heteroresistance</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>Genomic variations of
rdxA and
frxA genes in eight pairs of metronidazole (MTZ)-sensitive and -resistant isolates of
Helicobacter pylori were investigated. The paired strains from each single biopsy had identical
lspA-
glmM restriction fragment length polymorphism profiles, and the differences in MTZ susceptibility were mostly accounted for by mutations in the
rdxA gene. Truncation of RdxA is associated with a minimum inhibitory concentration of 64–128
mg/L. Early truncation of FrxA was observed both in susceptible and resistant isolates of seven paired strains. Inactivation of
frxA might play a significant role only in one low-level MTZ-resistant isolate where a missense mutation was found in the
rdxA gene.</description><subject>Amino Acid Sequence</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>frxA</subject><subject>Genes, Bacterial</subject><subject>Helicobacter Infections - drug therapy</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - drug effects</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Metronidazole - pharmacology</subject><subject>Metronidazole resistance</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nitroreductases - genetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>rdxA</subject><subject>Sequence Homology, Amino Acid</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O0zAQhy0EYsvCKyBzgFuCHcdxfKzKn0VawQXOlmNP2KmSuNjuLuV1eFFc2tVy5OSR9f1mRvMR8oqzmjPevd3WuLVLxhmd_V43jMma6Zpx_YiseK-aSmkuHpMV001b9VLpC_IspS1jXIpWPiUXvGubrud6RX6_-7ymCX7sYXFA7WKnQ8JEw0ij_7kuH56OsRRjDDPdWYzg6Qw5hgW9_RUmqBIsCTPewl-4ilDyuWxHr2BCFwbrMkS6O0whIsUUJpuhDBiyxQX8feOMsORE7zDf0BsoiXDfyMFz8mS0U4IX5_eSfPvw_uvmqrr-8vHTZn1duZbJXOmByWawquHeS8WHRvTOKdbaRrWCd5LzUWjd6066To9C9d5zrXzbKe240kJckjenvrsYykFSNjMmB9NkFwj7ZBRTTAihC6hPoIshpQij2UWcbTwYzszRkNmafwyZoyHDtCmGSvblech-mME_JM9KCvD6DNjk7DTGcgJMD5xqpe4FL9zmxEE5yS1CNMnh0aIvklw2PuB_rPMHL4W4Uw</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Matteo, Mario José</creator><creator>Pérez, Cecilia Valeria</creator><creator>Domingo, Mara Roxana</creator><creator>Olmos, Martín</creator><creator>Sanchez, Cristian</creator><creator>Catalano, Mariana</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060201</creationdate><title>DNA sequence analysis of rdxA and frxA from paired metronidazole-sensitive and -resistant Helicobacter pylori isolates obtained from patients with heteroresistance</title><author>Matteo, Mario José ; Pérez, Cecilia Valeria ; Domingo, Mara Roxana ; Olmos, Martín ; Sanchez, Cristian ; Catalano, Mariana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-9b052ba721dd571b238cc704a274316511f3998965c69f378dd197d4679c17933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>frxA</topic><topic>Genes, Bacterial</topic><topic>Helicobacter Infections - drug therapy</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - drug effects</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Metronidazole - pharmacology</topic><topic>Metronidazole resistance</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nitroreductases - genetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>rdxA</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matteo, Mario José</creatorcontrib><creatorcontrib>Pérez, Cecilia Valeria</creatorcontrib><creatorcontrib>Domingo, Mara Roxana</creatorcontrib><creatorcontrib>Olmos, Martín</creatorcontrib><creatorcontrib>Sanchez, Cristian</creatorcontrib><creatorcontrib>Catalano, Mariana</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matteo, Mario José</au><au>Pérez, Cecilia Valeria</au><au>Domingo, Mara Roxana</au><au>Olmos, Martín</au><au>Sanchez, Cristian</au><au>Catalano, Mariana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA sequence analysis of rdxA and frxA from paired metronidazole-sensitive and -resistant Helicobacter pylori isolates obtained from patients with heteroresistance</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>27</volume><issue>2</issue><spage>152</spage><epage>158</epage><pages>152-158</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>Genomic variations of
rdxA and
frxA genes in eight pairs of metronidazole (MTZ)-sensitive and -resistant isolates of
Helicobacter pylori were investigated. The paired strains from each single biopsy had identical
lspA-
glmM restriction fragment length polymorphism profiles, and the differences in MTZ susceptibility were mostly accounted for by mutations in the
rdxA gene. Truncation of RdxA is associated with a minimum inhibitory concentration of 64–128
mg/L. Early truncation of FrxA was observed both in susceptible and resistant isolates of seven paired strains. Inactivation of
frxA might play a significant role only in one low-level MTZ-resistant isolate where a missense mutation was found in the
rdxA gene.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>16426819</pmid><doi>10.1016/j.ijantimicag.2005.09.019</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Anti-Bacterial Agents - pharmacology Antibiotics. Antiinfectious agents. Antiparasitic agents Bacterial Proteins - genetics Biological and medical sciences DNA, Bacterial - genetics Drug Resistance, Bacterial - genetics frxA Genes, Bacterial Helicobacter Infections - drug therapy Helicobacter Infections - microbiology Helicobacter pylori Helicobacter pylori - drug effects Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Humans Medical sciences Metronidazole - pharmacology Metronidazole resistance Molecular Sequence Data Mutation Nitroreductases - genetics Pharmacology. Drug treatments Polymorphism, Restriction Fragment Length rdxA Sequence Homology, Amino Acid |
| title | DNA sequence analysis of rdxA and frxA from paired metronidazole-sensitive and -resistant Helicobacter pylori isolates obtained from patients with heteroresistance |
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