Spatiotemporal gene control by the Cre-ERT2 system in melanocytes
The organ‐specific and temporal control of gene activation/inactivation is a key issue in the understanding of protein function during normal and pathological development and during oncogenesis. We generated transgenic mice bearing a tamoxifen‐dependent Cre recombinase (Tyr::Cre‐ERT2) gene expressed...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2006-01, Vol.44 (1), p.34-43 |
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| container_title | Genesis (New York, N.Y. : 2000) |
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| creator | Yajima, Ichiro Belloir, Elodie Bourgeois, Yveline Kumasaka, Mayuko Delmas, Véronique Larue, Lionel |
| description | The organ‐specific and temporal control of gene activation/inactivation is a key issue in the understanding of protein function during normal and pathological development and during oncogenesis. We generated transgenic mice bearing a tamoxifen‐dependent Cre recombinase (Tyr::Cre‐ERT2) gene expressed under the control of a 6.1 kb murine tyrosinase promoter in order to facilitate targeted spatiotemporally controlled somatic recombination in melanoblasts/melanocytes. Cre‐ERT2 production was detected in tissues containing melanocytes. After tamoxifen induction at various times during embryogenesis and adulthood in a Cre‐responsive reporter mouse strain, genetic recombination was detected in the melanoblasts and melanocytes of the skin. Thus, the Tyr::Cre‐ERT2 transgenic mice provides a valuable tool for following this cell lineage and for investigating gene function in melanocyte development and transformation. genesis 44:34–43, 2006. © 2006 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/gene.20182 |
| format | Article |
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We generated transgenic mice bearing a tamoxifen‐dependent Cre recombinase (Tyr::Cre‐ERT2) gene expressed under the control of a 6.1 kb murine tyrosinase promoter in order to facilitate targeted spatiotemporally controlled somatic recombination in melanoblasts/melanocytes. Cre‐ERT2 production was detected in tissues containing melanocytes. After tamoxifen induction at various times during embryogenesis and adulthood in a Cre‐responsive reporter mouse strain, genetic recombination was detected in the melanoblasts and melanocytes of the skin. Thus, the Tyr::Cre‐ERT2 transgenic mice provides a valuable tool for following this cell lineage and for investigating gene function in melanocyte development and transformation. genesis 44:34–43, 2006. © 2006 Wiley‐Liss, Inc.</description><identifier>ISSN: 1526-954X</identifier><identifier>EISSN: 1526-968X</identifier><identifier>DOI: 10.1002/gene.20182</identifier><identifier>PMID: 16419042</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; beta-Galactosidase - genetics ; conditional mutagenesis ; development ; Gene Expression Regulation - physiology ; Integrases - physiology ; melanoblast ; Melanocytes - drug effects ; Melanocytes - metabolism ; Mice ; Mice, Transgenic ; Monophenol Monooxygenase - genetics ; mouse ; Promoter Regions, Genetic ; skin ; Tamoxifen - pharmacology ; Transcriptional Activation ; transformation ; tyrosinase ; vitiligo</subject><ispartof>Genesis (New York, N.Y. : 2000), 2006-01, Vol.44 (1), p.34-43</ispartof><rights>Copyright © 2006 Wiley‐Liss, Inc.</rights><rights>(c) 2006 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4622-7d99ffd5344abd72be846852912d3814bafd3f701c598cb11b14ee7d3b961cd83</citedby><cites>FETCH-LOGICAL-c4622-7d99ffd5344abd72be846852912d3814bafd3f701c598cb11b14ee7d3b961cd83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fgene.20182$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fgene.20182$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16419042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yajima, Ichiro</creatorcontrib><creatorcontrib>Belloir, Elodie</creatorcontrib><creatorcontrib>Bourgeois, Yveline</creatorcontrib><creatorcontrib>Kumasaka, Mayuko</creatorcontrib><creatorcontrib>Delmas, Véronique</creatorcontrib><creatorcontrib>Larue, Lionel</creatorcontrib><title>Spatiotemporal gene control by the Cre-ERT2 system in melanocytes</title><title>Genesis (New York, N.Y. : 2000)</title><addtitle>Genesis</addtitle><description>The organ‐specific and temporal control of gene activation/inactivation is a key issue in the understanding of protein function during normal and pathological development and during oncogenesis. We generated transgenic mice bearing a tamoxifen‐dependent Cre recombinase (Tyr::Cre‐ERT2) gene expressed under the control of a 6.1 kb murine tyrosinase promoter in order to facilitate targeted spatiotemporally controlled somatic recombination in melanoblasts/melanocytes. Cre‐ERT2 production was detected in tissues containing melanocytes. After tamoxifen induction at various times during embryogenesis and adulthood in a Cre‐responsive reporter mouse strain, genetic recombination was detected in the melanoblasts and melanocytes of the skin. Thus, the Tyr::Cre‐ERT2 transgenic mice provides a valuable tool for following this cell lineage and for investigating gene function in melanocyte development and transformation. genesis 44:34–43, 2006. © 2006 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>beta-Galactosidase - genetics</subject><subject>conditional mutagenesis</subject><subject>development</subject><subject>Gene Expression Regulation - physiology</subject><subject>Integrases - physiology</subject><subject>melanoblast</subject><subject>Melanocytes - drug effects</subject><subject>Melanocytes - metabolism</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Monophenol Monooxygenase - genetics</subject><subject>mouse</subject><subject>Promoter Regions, Genetic</subject><subject>skin</subject><subject>Tamoxifen - pharmacology</subject><subject>Transcriptional Activation</subject><subject>transformation</subject><subject>tyrosinase</subject><subject>vitiligo</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwkAQQDdGI4he_AGmJw8mxZ3ttrt7JIhgQtAARm6bfky12lLcLdH-e4uA3vQ0c3jzMnmEnAPtAqXs-hmX2GUUJDsgbfBZ4KpALg73u88XLXJi7Sul1JeMHZMWBBwU5axNerNVWGVlhcWqNGHubFxOXC4rU-ZOVDvVCzp9g-5gOmeOrW0DOtnSKTAPl2VcV2hPyVEa5hbPdrNDHm8H8_7IHd8P7_q9sRvzgDFXJEqlaeJ7nIdRIliEkgfSZwpY4kngUZgmXiooxL6ScQQQAUcUiRepAOJEeh1yufWuTPm-RlvpIrMx5s0jWK6tFjRQAgT7F4SG5JJuwKstGJvSWoOpXpmsCE2tgepNWb2pob_LNvDFzrqOCkx-0V3KBoAt8JHlWP-h0sPBZLCXutubrAn7-XMTmjcdCE_4-mky1DejoXqY-mM9874AtFSRwA</recordid><startdate>200601</startdate><enddate>200601</enddate><creator>Yajima, Ichiro</creator><creator>Belloir, Elodie</creator><creator>Bourgeois, Yveline</creator><creator>Kumasaka, Mayuko</creator><creator>Delmas, Véronique</creator><creator>Larue, Lionel</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200601</creationdate><title>Spatiotemporal gene control by the Cre-ERT2 system in melanocytes</title><author>Yajima, Ichiro ; 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals beta-Galactosidase - genetics conditional mutagenesis development Gene Expression Regulation - physiology Integrases - physiology melanoblast Melanocytes - drug effects Melanocytes - metabolism Mice Mice, Transgenic Monophenol Monooxygenase - genetics mouse Promoter Regions, Genetic skin Tamoxifen - pharmacology Transcriptional Activation transformation tyrosinase vitiligo |
| title | Spatiotemporal gene control by the Cre-ERT2 system in melanocytes |
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