Production and Processing of Milk from Transgenic Goats Expressing Human Lysozyme in the Mammary Gland
The potential for applying biotechnology to benefit animal agriculture and food production has long been speculated. The addition of human milk components with intrinsic antimicrobial activity and positive charge to livestock milk by genetic engineering has the potential to benefit animal health, as...
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| Veröffentlicht in: | Journal of dairy science 2006-02, Vol.89 (2), p.518-524 |
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| creator | Maga, E. A Shoemaker, C. F Rowe, J. D BonDurant, R. H Anderson, G. B Murray, J. D |
| description | The potential for applying biotechnology to benefit animal agriculture and food production has long been speculated. The addition of human milk components with intrinsic antimicrobial activity and positive charge to livestock milk by genetic engineering has the potential to benefit animal health, as well as food safety and production. We generated one line of transgenic goats as a model for the dairy cow designed to express human lysozyme in the mammary gland. Here we report the characterization of the milk from 5 transgenic females of this line expressing human lysozyme in their milk at 270μg/mL or 68% of the level found in human milk. Milk from transgenic animals had a lower somatic cell count, but the overall component composition of the milk and milk production were not different from controls. Milk from transgenic animals had a shorter rennet clotting time and increased curd strength. Milk of such nature may be of benefit to the producer by influencing udder health and milk processing. |
| doi_str_mv | 10.3168/jds.s0022-0302(06)72114-2 |
| format | Article |
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A ; Shoemaker, C. F ; Rowe, J. D ; BonDurant, R. H ; Anderson, G. B ; Murray, J. D</creator><creatorcontrib>Maga, E. A ; Shoemaker, C. F ; Rowe, J. D ; BonDurant, R. H ; Anderson, G. B ; Murray, J. D</creatorcontrib><description>The potential for applying biotechnology to benefit animal agriculture and food production has long been speculated. The addition of human milk components with intrinsic antimicrobial activity and positive charge to livestock milk by genetic engineering has the potential to benefit animal health, as well as food safety and production. We generated one line of transgenic goats as a model for the dairy cow designed to express human lysozyme in the mammary gland. Here we report the characterization of the milk from 5 transgenic females of this line expressing human lysozyme in their milk at 270μg/mL or 68% of the level found in human milk. Milk from transgenic animals had a lower somatic cell count, but the overall component composition of the milk and milk production were not different from controls. Milk from transgenic animals had a shorter rennet clotting time and increased curd strength. Milk of such nature may be of benefit to the producer by influencing udder health and milk processing.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.s0022-0302(06)72114-2</identifier><identifier>PMID: 16428620</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>Savoy, IL: Elsevier Inc</publisher><subject>Animal productions ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - physiology ; Anti-Infective Agents ; antibacterial proteins ; Biological and medical sciences ; Cell Count ; Chymosin - metabolism ; Female ; Food Handling - methods ; Food industries ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; goat milk ; Goats - genetics ; Goats - physiology ; Humans ; Lactation ; lysozyme ; Mammary Glands, Animal - enzymology ; milk ; Milk - chemistry ; Milk - cytology ; Milk and cheese industries. Ice creams ; milk composition ; milk yield ; Muramidase - genetics ; Terrestrial animal productions ; transgenic ; transgenic animals ; udder health ; Vertebrates</subject><ispartof>Journal of dairy science, 2006-02, Vol.89 (2), p.518-524</ispartof><rights>2006 American Dairy Science Association</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Dairy Science Association Feb 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c634t-38fb33c0b8a392d395ef38769d65a5aacc0e7e0b802173f054eee0569c9e14bf3</citedby><cites>FETCH-LOGICAL-c634t-38fb33c0b8a392d395ef38769d65a5aacc0e7e0b802173f054eee0569c9e14bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022030206721142$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17442370$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16428620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maga, E. A</creatorcontrib><creatorcontrib>Shoemaker, C. F</creatorcontrib><creatorcontrib>Rowe, J. D</creatorcontrib><creatorcontrib>BonDurant, R. H</creatorcontrib><creatorcontrib>Anderson, G. B</creatorcontrib><creatorcontrib>Murray, J. D</creatorcontrib><title>Production and Processing of Milk from Transgenic Goats Expressing Human Lysozyme in the Mammary Gland</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>The potential for applying biotechnology to benefit animal agriculture and food production has long been speculated. The addition of human milk components with intrinsic antimicrobial activity and positive charge to livestock milk by genetic engineering has the potential to benefit animal health, as well as food safety and production. We generated one line of transgenic goats as a model for the dairy cow designed to express human lysozyme in the mammary gland. Here we report the characterization of the milk from 5 transgenic females of this line expressing human lysozyme in their milk at 270μg/mL or 68% of the level found in human milk. Milk from transgenic animals had a lower somatic cell count, but the overall component composition of the milk and milk production were not different from controls. Milk from transgenic animals had a shorter rennet clotting time and increased curd strength. Milk of such nature may be of benefit to the producer by influencing udder health and milk processing.</description><subject>Animal productions</subject><subject>Animals</subject><subject>Animals, Genetically Modified - genetics</subject><subject>Animals, Genetically Modified - physiology</subject><subject>Anti-Infective Agents</subject><subject>antibacterial proteins</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Chymosin - metabolism</subject><subject>Female</subject><subject>Food Handling - methods</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>goat milk</subject><subject>Goats - genetics</subject><subject>Goats - physiology</subject><subject>Humans</subject><subject>Lactation</subject><subject>lysozyme</subject><subject>Mammary Glands, Animal - enzymology</subject><subject>milk</subject><subject>Milk - chemistry</subject><subject>Milk - cytology</subject><subject>Milk and cheese industries. Ice creams</subject><subject>milk composition</subject><subject>milk yield</subject><subject>Muramidase - genetics</subject><subject>Terrestrial animal productions</subject><subject>transgenic</subject><subject>transgenic animals</subject><subject>udder health</subject><subject>Vertebrates</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkdFu0zAUhiMEYmPwCmCQQHCRcWzHTnyJqtEhdQJp27XlOsetSxIXOwHK0-PSiiFu4Mo68uf_-NdXFM8pnHMqm7ebNp0nAMZK4MBeg3xTM0qrkt0rTqlgouRUNfeL09_ISfEopU0eKQPxsDihsmKNZHBauE8xtJMdfRiIGVqSR4sp-WFFgiNXvvtMXAw9uYlmSCscvCXzYMZELr5v4xG8nHozkMUuhR-7HokfyLhGcmX63sQdmXc593HxwJku4ZPjeVbcvr-4mV2Wi4_zD7N3i9JKXo0lb9yScwvLxnDFWq4EOt7UUrVSGGGMtYA15uvcpOYORIWIIKSyCmm1dPyseHXI3cbwZcI06t4ni13-A4Yp6RqkEpWs_gkygJpDIzP44i9wE6Y45BKaKlErlbdnSB0gG0NKEZ3eRr9vrynovTKdlenrvQ-996FB6l_KNMtvnx4XTMse27uXR0cZeHkETLKmc9mE9emOq6uK8foPbu1X628-ok696bocS_frG6WZFrTJ3LMD50zQZhVz1u01A8qBggDO6kzMDgRmVV89Rp2sx8Fim1PtqNvg_6PYTw98y2I</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Maga, E. 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Psychology</topic><topic>Gene Expression</topic><topic>goat milk</topic><topic>Goats - genetics</topic><topic>Goats - physiology</topic><topic>Humans</topic><topic>Lactation</topic><topic>lysozyme</topic><topic>Mammary Glands, Animal - enzymology</topic><topic>milk</topic><topic>Milk - chemistry</topic><topic>Milk - cytology</topic><topic>Milk and cheese industries. Ice creams</topic><topic>milk composition</topic><topic>milk yield</topic><topic>Muramidase - genetics</topic><topic>Terrestrial animal productions</topic><topic>transgenic</topic><topic>transgenic animals</topic><topic>udder health</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maga, E. A</creatorcontrib><creatorcontrib>Shoemaker, C. F</creatorcontrib><creatorcontrib>Rowe, J. D</creatorcontrib><creatorcontrib>BonDurant, R. H</creatorcontrib><creatorcontrib>Anderson, G. B</creatorcontrib><creatorcontrib>Murray, J. 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| subjects | Animal productions Animals Animals, Genetically Modified - genetics Animals, Genetically Modified - physiology Anti-Infective Agents antibacterial proteins Biological and medical sciences Cell Count Chymosin - metabolism Female Food Handling - methods Food industries Fundamental and applied biological sciences. Psychology Gene Expression goat milk Goats - genetics Goats - physiology Humans Lactation lysozyme Mammary Glands, Animal - enzymology milk Milk - chemistry Milk - cytology Milk and cheese industries. Ice creams milk composition milk yield Muramidase - genetics Terrestrial animal productions transgenic transgenic animals udder health Vertebrates |
| title | Production and Processing of Milk from Transgenic Goats Expressing Human Lysozyme in the Mammary Gland |
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