Evidence for quorum sensing in Clostridium botulinum 56A
Aims: Experiments were designed to detect quorum‐sensing signals produced by Clostridium botulinum. Methods and Results: Clostridium botulinum 56A cell‐free supernatants obtained at the end of lag phase, the mid‐exponential phase and early stationary phase of growth were assayed for bioluminescenc...
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| Veröffentlicht in: | Letters in applied microbiology 2006-01, Vol.42 (1), p.54-58 |
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| creator | Zhao, L. Montville, T.J. Schaffner, D.W. |
| description | Aims: Experiments were designed to detect quorum‐sensing signals produced by Clostridium botulinum.
Methods and Results: Clostridium botulinum 56A cell‐free supernatants obtained at the end of lag phase, the mid‐exponential phase and early stationary phase of growth were assayed for bioluminescence in the Vibrio harveyi quorum‐sensing assay system. Twelve and 16‐h culture supernatants induced bioluminescence in the auto‐inducer 2 (AI‐2) but not the auto‐inducer 1 (AI‐1) assay. Intra‐species quorum sensing was also assayed as the ability of the supernatants to promote spore germination and outgrowth in a microtitre plate system. Spore populations exposed to C. botulinum supernatant from the end of lag phase became positive for growth sooner than controls.
Conclusions: The influence of cell‐free supernatant on ungerminated spores and detection of bioluminescence in the AI‐2 assay are evidence for a signalling molecule(s) and provide a first step in characterizing C. botulinum quorum sensing.
Significance and Impact of the Study: This study suggests that spores do not behave independently of each other and may explain the inocula size effects observed in challenge studies. Whether AI‐2 production in C. botulinum serves as an inter‐species signal or as a detoxification mechanism remains to be determined. |
| doi_str_mv | 10.1111/j.1472-765X.2005.01807.x |
| format | Article |
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Methods and Results: Clostridium botulinum 56A cell‐free supernatants obtained at the end of lag phase, the mid‐exponential phase and early stationary phase of growth were assayed for bioluminescence in the Vibrio harveyi quorum‐sensing assay system. Twelve and 16‐h culture supernatants induced bioluminescence in the auto‐inducer 2 (AI‐2) but not the auto‐inducer 1 (AI‐1) assay. Intra‐species quorum sensing was also assayed as the ability of the supernatants to promote spore germination and outgrowth in a microtitre plate system. Spore populations exposed to C. botulinum supernatant from the end of lag phase became positive for growth sooner than controls.
Conclusions: The influence of cell‐free supernatant on ungerminated spores and detection of bioluminescence in the AI‐2 assay are evidence for a signalling molecule(s) and provide a first step in characterizing C. botulinum quorum sensing.
Significance and Impact of the Study: This study suggests that spores do not behave independently of each other and may explain the inocula size effects observed in challenge studies. Whether AI‐2 production in C. botulinum serves as an inter‐species signal or as a detoxification mechanism remains to be determined.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>EISSN: 1365-2673</identifier><identifier>DOI: 10.1111/j.1472-765X.2005.01807.x</identifier><identifier>PMID: 16411920</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - analysis ; autoinducer‐1 ; autoinducer‐2 ; Biological and medical sciences ; bioluminescence ; Clostridium botulinum ; Clostridium botulinum - growth & development ; Clostridium botulinum - physiology ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Homoserine - analogs & derivatives ; Homoserine - analysis ; Lactones - analysis ; Luminescent Measurements - methods ; Microbiology ; Signal Transduction ; spores ; Spores, Bacterial - drug effects ; Spores, Bacterial - growth & development ; Vibrio - chemistry ; Vibrio harveyi</subject><ispartof>Letters in applied microbiology, 2006-01, Vol.42 (1), p.54-58</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4837-9c52874f313f81b7d6ceadab7cd3530cf1ce59205462627a2858d23d6ba8c24d3</citedby><cites>FETCH-LOGICAL-c4837-9c52874f313f81b7d6ceadab7cd3530cf1ce59205462627a2858d23d6ba8c24d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2005.01807.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2005.01807.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4009,27902,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17557415$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16411920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, L.</creatorcontrib><creatorcontrib>Montville, T.J.</creatorcontrib><creatorcontrib>Schaffner, D.W.</creatorcontrib><title>Evidence for quorum sensing in Clostridium botulinum 56A</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>Aims: Experiments were designed to detect quorum‐sensing signals produced by Clostridium botulinum.
Methods and Results: Clostridium botulinum 56A cell‐free supernatants obtained at the end of lag phase, the mid‐exponential phase and early stationary phase of growth were assayed for bioluminescence in the Vibrio harveyi quorum‐sensing assay system. Twelve and 16‐h culture supernatants induced bioluminescence in the auto‐inducer 2 (AI‐2) but not the auto‐inducer 1 (AI‐1) assay. Intra‐species quorum sensing was also assayed as the ability of the supernatants to promote spore germination and outgrowth in a microtitre plate system. Spore populations exposed to C. botulinum supernatant from the end of lag phase became positive for growth sooner than controls.
Conclusions: The influence of cell‐free supernatant on ungerminated spores and detection of bioluminescence in the AI‐2 assay are evidence for a signalling molecule(s) and provide a first step in characterizing C. botulinum quorum sensing.
Significance and Impact of the Study: This study suggests that spores do not behave independently of each other and may explain the inocula size effects observed in challenge studies. Whether AI‐2 production in C. botulinum serves as an inter‐species signal or as a detoxification mechanism remains to be determined.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - analysis</subject><subject>autoinducer‐1</subject><subject>autoinducer‐2</subject><subject>Biological and medical sciences</subject><subject>bioluminescence</subject><subject>Clostridium botulinum</subject><subject>Clostridium botulinum - growth & development</subject><subject>Clostridium botulinum - physiology</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Homoserine - analogs & derivatives</subject><subject>Homoserine - analysis</subject><subject>Lactones - analysis</subject><subject>Luminescent Measurements - methods</subject><subject>Microbiology</subject><subject>Signal Transduction</subject><subject>spores</subject><subject>Spores, Bacterial - drug effects</subject><subject>Spores, Bacterial - growth & development</subject><subject>Vibrio - chemistry</subject><subject>Vibrio harveyi</subject><issn>0266-8254</issn><issn>1472-765X</issn><issn>1365-2673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkLtOwzAUhi0EoqXwCigLbAm-2xkYqqpcpCIWkNgsx3aQqzRp7QbatydpKzqCFx-d8x371wdAgmCGunM3zxAVOBWcfWQYQpZBJKHINidg-Ds4BUOIOU8lZnQALmKcQwglwvk5GCBOEcoxHAI5_fLW1cYlZROSVduEdpFEV0dffya-TiZVE9fBW9-1i2bdVr7uKsbHl-Cs1FV0V4d7BN4fpm-Tp3T2-vg8Gc9SQyURaW4YloKWBJFSokJYbpy2uhDGEkagKZFxrEvCKMccC40lkxYTywstDaaWjMDt_t1laFati2u18NG4qtK1a9qoBOQ5JYj9CaJcklxK3oFyD5rQxBhcqZbBL3TYKgRVr1fNVW9R9RZVr1ft9KpNt3p9-KMtFs4eFw8-O-DmAOhodFUGXRsfj5xgTNBd2Ps99-0rt_13ADUbv_QV-QF3TpSm</recordid><startdate>200601</startdate><enddate>200601</enddate><creator>Zhao, L.</creator><creator>Montville, T.J.</creator><creator>Schaffner, D.W.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200601</creationdate><title>Evidence for quorum sensing in Clostridium botulinum 56A</title><author>Zhao, L. ; Montville, T.J. ; Schaffner, D.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4837-9c52874f313f81b7d6ceadab7cd3530cf1ce59205462627a2858d23d6ba8c24d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - analysis</topic><topic>autoinducer‐1</topic><topic>autoinducer‐2</topic><topic>Biological and medical sciences</topic><topic>bioluminescence</topic><topic>Clostridium botulinum</topic><topic>Clostridium botulinum - growth & development</topic><topic>Clostridium botulinum - physiology</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Homoserine - analogs & derivatives</topic><topic>Homoserine - analysis</topic><topic>Lactones - analysis</topic><topic>Luminescent Measurements - methods</topic><topic>Microbiology</topic><topic>Signal Transduction</topic><topic>spores</topic><topic>Spores, Bacterial - drug effects</topic><topic>Spores, Bacterial - growth & development</topic><topic>Vibrio - chemistry</topic><topic>Vibrio harveyi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, L.</creatorcontrib><creatorcontrib>Montville, T.J.</creatorcontrib><creatorcontrib>Schaffner, D.W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, L.</au><au>Montville, T.J.</au><au>Schaffner, D.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for quorum sensing in Clostridium botulinum 56A</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2006-01</date><risdate>2006</risdate><volume>42</volume><issue>1</issue><spage>54</spage><epage>58</epage><pages>54-58</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><eissn>1365-2673</eissn><coden>LAMIE7</coden><abstract>Aims: Experiments were designed to detect quorum‐sensing signals produced by Clostridium botulinum.
Methods and Results: Clostridium botulinum 56A cell‐free supernatants obtained at the end of lag phase, the mid‐exponential phase and early stationary phase of growth were assayed for bioluminescence in the Vibrio harveyi quorum‐sensing assay system. Twelve and 16‐h culture supernatants induced bioluminescence in the auto‐inducer 2 (AI‐2) but not the auto‐inducer 1 (AI‐1) assay. Intra‐species quorum sensing was also assayed as the ability of the supernatants to promote spore germination and outgrowth in a microtitre plate system. Spore populations exposed to C. botulinum supernatant from the end of lag phase became positive for growth sooner than controls.
Conclusions: The influence of cell‐free supernatant on ungerminated spores and detection of bioluminescence in the AI‐2 assay are evidence for a signalling molecule(s) and provide a first step in characterizing C. botulinum quorum sensing.
Significance and Impact of the Study: This study suggests that spores do not behave independently of each other and may explain the inocula size effects observed in challenge studies. Whether AI‐2 production in C. botulinum serves as an inter‐species signal or as a detoxification mechanism remains to be determined.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16411920</pmid><doi>10.1111/j.1472-765X.2005.01807.x</doi><tpages>5</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - analysis autoinducer‐1 autoinducer‐2 Biological and medical sciences bioluminescence Clostridium botulinum Clostridium botulinum - growth & development Clostridium botulinum - physiology Culture Media Fundamental and applied biological sciences. Psychology Homoserine - analogs & derivatives Homoserine - analysis Lactones - analysis Luminescent Measurements - methods Microbiology Signal Transduction spores Spores, Bacterial - drug effects Spores, Bacterial - growth & development Vibrio - chemistry Vibrio harveyi |
| title | Evidence for quorum sensing in Clostridium botulinum 56A |
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