Role of Matrix Metalloproteinases in HaCaT Keratinocytes Apoptosis Induced by Loxosceles Venom Sphingomyelinase D
Envenomation by the spider Loxosceles (brown spider) can result in dermonecrosis and severe ulceration. We have previously shown that Loxosceles sphingomyelinase D (SMaseD), the enzyme responsible for these pathological effects, induced expression of matrix metalloproteinase-9 (MMP-9), which is poss...
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| Veröffentlicht in: | Journal of investigative dermatology 2006-01, Vol.126 (1), p.61-68 |
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| creator | Paixão-Cavalcante, Danielle van den Berg, Carmen W. de Freitas Fernandes-Pedrosa, Matheus Gonçalves de Andrade, Rute M. Tambourgi, Denise V. |
| description | Envenomation by the spider Loxosceles (brown spider) can result in dermonecrosis and severe ulceration. We have previously shown that Loxosceles sphingomyelinase D (SMaseD), the enzyme responsible for these pathological effects, induced expression of matrix metalloproteinase-9 (MMP-9), which is possibly one of the main factors involved in the pathogenesis of the cutaneous loxoscelism. The aim of this study was to further investigate the molecular mechanisms triggered by Loxosceles SMaseD involved in the initiation of the dermonecrotic lesion, using HaCaT cultures, a human keratinocyte cell line, as an in vitro model for cutaneous loxoscelism. We show here that SMaseD from Loxosceles spider venom induces apoptosis in human keratinocytes, which is associated with an increased expression of metalloproteinase-2 and -9, and that the use of metalloproteinase inhibitors, such as tetracycline, may prevent cell death and potentially may prevent tissue destruction after envenomation. |
| doi_str_mv | 10.1038/sj.jid.5700049 |
| format | Article |
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We have previously shown that Loxosceles sphingomyelinase D (SMaseD), the enzyme responsible for these pathological effects, induced expression of matrix metalloproteinase-9 (MMP-9), which is possibly one of the main factors involved in the pathogenesis of the cutaneous loxoscelism. The aim of this study was to further investigate the molecular mechanisms triggered by Loxosceles SMaseD involved in the initiation of the dermonecrotic lesion, using HaCaT cultures, a human keratinocyte cell line, as an in vitro model for cutaneous loxoscelism. We show here that SMaseD from Loxosceles spider venom induces apoptosis in human keratinocytes, which is associated with an increased expression of metalloproteinase-2 and -9, and that the use of metalloproteinase inhibitors, such as tetracycline, may prevent cell death and potentially may prevent tissue destruction after envenomation.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1038/sj.jid.5700049</identifier><identifier>PMID: 16417218</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Annexin A5 - metabolism ; Apoptosis ; Biomarkers - analysis ; Cell Line, Transformed ; Humans ; Keratinocytes - drug effects ; Keratinocytes - enzymology ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 9 - metabolism ; Phosphoric Diester Hydrolases - pharmacology ; Propidium - metabolism ; Spider Venoms - enzymology</subject><ispartof>Journal of investigative dermatology, 2006-01, Vol.126 (1), p.61-68</ispartof><rights>2006 The Society for Investigative Dermatology, Inc</rights><rights>Copyright Nature Publishing Group Jan 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-f87876bbb2537e6dd7533263e4180f9597cd6033c1aa1c9e809fb7bb95d347e73</citedby><cites>FETCH-LOGICAL-c409t-f87876bbb2537e6dd7533263e4180f9597cd6033c1aa1c9e809fb7bb95d347e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/210377925?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,64390,64392,64394,72474</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16417218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paixão-Cavalcante, Danielle</creatorcontrib><creatorcontrib>van den Berg, Carmen W.</creatorcontrib><creatorcontrib>de Freitas Fernandes-Pedrosa, Matheus</creatorcontrib><creatorcontrib>Gonçalves de Andrade, Rute M.</creatorcontrib><creatorcontrib>Tambourgi, Denise V.</creatorcontrib><title>Role of Matrix Metalloproteinases in HaCaT Keratinocytes Apoptosis Induced by Loxosceles Venom Sphingomyelinase D</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Envenomation by the spider Loxosceles (brown spider) can result in dermonecrosis and severe ulceration. 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| subjects | Annexin A5 - metabolism Apoptosis Biomarkers - analysis Cell Line, Transformed Humans Keratinocytes - drug effects Keratinocytes - enzymology Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - metabolism Phosphoric Diester Hydrolases - pharmacology Propidium - metabolism Spider Venoms - enzymology |
| title | Role of Matrix Metalloproteinases in HaCaT Keratinocytes Apoptosis Induced by Loxosceles Venom Sphingomyelinase D |
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