Distribution of mRNA coding for 5-hydroxytryptamine receptor subtypes in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation
To investigate the distribution of mRNA coding for 7 subtypes of 5-hydroxytryptamine receptors (5-HTRs) in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation (CDD). Full-thickness intestinal wall biopsy specimens were obtained from the ileum, cecum, proximal loop o...
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| Veröffentlicht in: | American journal of veterinary research 2006, Vol.67 (1), p.95-101 |
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| creator | Engel, L Kobel, B Ontsouka, E.C Graber, H.U Blum, J.W Steiner, A Meylan, M |
| description | To investigate the distribution of mRNA coding for 7 subtypes of 5-hydroxytryptamine receptors (5-HTRs) in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation (CDD).
Full-thickness intestinal wall biopsy specimens were obtained from the ileum, cecum, proximal loop of the ascending colon, and external loop of the spiral colon (ELSC) of 15 cows with CDD (group 1) and 15 healthy dairy cows allocated to 2 control groups (specimens collected during routine laparotomy [group 2] or after cows were slaughtered [group 3]).
Amounts of mRNA coding for 7 subtypes of 5-HTRs (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1F, 5-HT2A, 5-HT2B, and 5-HT4) were measured by quantitative real-time reverse transcriptase-PCR assay. Results were expressed as the percentage of mRNA expression of a housekeeping gene.
Expression of mRNA coding for 5-HTR1B, 5-HTR2B, and 5-HTR4 was significantly lower in cows with CDD than in healthy cows. For 5-HTR2B and 5-HTR4, significant differences between cows with CDD and control cows were most pronounced for the ELSC. Expression of mRNA for 5-HTR1D, 5-HTR1F, and 5-HTR2A was extremely low in all groups, and mRNA for 5-HTR1A was not detected.
Relative concentrations of mRNA coding for 5-HTR1B, 5-HT2B, and 5-HTR4 were significantly lower in the intestines of cows with CDD than in the intestines of healthy dairy cows, especially for 5-HT2B and 5-HTR4 in the ELSC. This supports the hypothesis that serotonergic mechanisms, primarily in the spiral colon, are implicated in the pathogenesis of CDD. |
| doi_str_mv | 10.2460/ajvr.67.1.95 |
| format | Article |
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Full-thickness intestinal wall biopsy specimens were obtained from the ileum, cecum, proximal loop of the ascending colon, and external loop of the spiral colon (ELSC) of 15 cows with CDD (group 1) and 15 healthy dairy cows allocated to 2 control groups (specimens collected during routine laparotomy [group 2] or after cows were slaughtered [group 3]).
Amounts of mRNA coding for 7 subtypes of 5-HTRs (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1F, 5-HT2A, 5-HT2B, and 5-HT4) were measured by quantitative real-time reverse transcriptase-PCR assay. Results were expressed as the percentage of mRNA expression of a housekeeping gene.
Expression of mRNA coding for 5-HTR1B, 5-HTR2B, and 5-HTR4 was significantly lower in cows with CDD than in healthy cows. For 5-HTR2B and 5-HTR4, significant differences between cows with CDD and control cows were most pronounced for the ELSC. Expression of mRNA for 5-HTR1D, 5-HTR1F, and 5-HTR2A was extremely low in all groups, and mRNA for 5-HTR1A was not detected.
Relative concentrations of mRNA coding for 5-HTR1B, 5-HT2B, and 5-HTR4 were significantly lower in the intestines of cows with CDD than in the intestines of healthy dairy cows, especially for 5-HT2B and 5-HTR4 in the ELSC. This supports the hypothesis that serotonergic mechanisms, primarily in the spiral colon, are implicated in the pathogenesis of CDD.</description><identifier>ISSN: 0002-9645</identifier><identifier>EISSN: 1943-5681</identifier><identifier>DOI: 10.2460/ajvr.67.1.95</identifier><identifier>PMID: 16426218</identifier><language>eng</language><publisher>United States</publisher><subject>Analysis of Variance ; Animals ; Cattle ; cattle diseases ; Cattle Diseases - metabolism ; cecal dilatation-dislocation disease ; Cecal Diseases - metabolism ; Cecal Diseases - veterinary ; cecum ; colon ; dairy cows ; digestive system diseases ; Dilatation, Pathologic - veterinary ; Female ; gastrointestinal motility ; gene expression ; Genetic Variation ; ileum ; Intestines - metabolism ; Intestines - surgery ; messenger RNA ; pathogenesis ; Protein Isoforms - metabolism ; receptors ; Receptors, Serotonin - genetics ; Receptors, Serotonin - metabolism ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; serotonin</subject><ispartof>American journal of veterinary research, 2006, Vol.67 (1), p.95-101</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-e88be473b30eaa0f1451e904cd0df62c74f86cd1b5529a272ebdf57b8db52b543</citedby><cites>FETCH-LOGICAL-c351t-e88be473b30eaa0f1451e904cd0df62c74f86cd1b5529a272ebdf57b8db52b543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16426218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Engel, L</creatorcontrib><creatorcontrib>Kobel, B</creatorcontrib><creatorcontrib>Ontsouka, E.C</creatorcontrib><creatorcontrib>Graber, H.U</creatorcontrib><creatorcontrib>Blum, J.W</creatorcontrib><creatorcontrib>Steiner, A</creatorcontrib><creatorcontrib>Meylan, M</creatorcontrib><title>Distribution of mRNA coding for 5-hydroxytryptamine receptor subtypes in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation</title><title>American journal of veterinary research</title><addtitle>Am J Vet Res</addtitle><description>To investigate the distribution of mRNA coding for 7 subtypes of 5-hydroxytryptamine receptors (5-HTRs) in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation (CDD).
Full-thickness intestinal wall biopsy specimens were obtained from the ileum, cecum, proximal loop of the ascending colon, and external loop of the spiral colon (ELSC) of 15 cows with CDD (group 1) and 15 healthy dairy cows allocated to 2 control groups (specimens collected during routine laparotomy [group 2] or after cows were slaughtered [group 3]).
Amounts of mRNA coding for 7 subtypes of 5-HTRs (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1F, 5-HT2A, 5-HT2B, and 5-HT4) were measured by quantitative real-time reverse transcriptase-PCR assay. Results were expressed as the percentage of mRNA expression of a housekeeping gene.
Expression of mRNA coding for 5-HTR1B, 5-HTR2B, and 5-HTR4 was significantly lower in cows with CDD than in healthy cows. For 5-HTR2B and 5-HTR4, significant differences between cows with CDD and control cows were most pronounced for the ELSC. Expression of mRNA for 5-HTR1D, 5-HTR1F, and 5-HTR2A was extremely low in all groups, and mRNA for 5-HTR1A was not detected.
Relative concentrations of mRNA coding for 5-HTR1B, 5-HT2B, and 5-HTR4 were significantly lower in the intestines of cows with CDD than in the intestines of healthy dairy cows, especially for 5-HT2B and 5-HTR4 in the ELSC. This supports the hypothesis that serotonergic mechanisms, primarily in the spiral colon, are implicated in the pathogenesis of CDD.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Cattle</subject><subject>cattle diseases</subject><subject>Cattle Diseases - metabolism</subject><subject>cecal dilatation-dislocation disease</subject><subject>Cecal Diseases - metabolism</subject><subject>Cecal Diseases - veterinary</subject><subject>cecum</subject><subject>colon</subject><subject>dairy cows</subject><subject>digestive system diseases</subject><subject>Dilatation, Pathologic - veterinary</subject><subject>Female</subject><subject>gastrointestinal motility</subject><subject>gene expression</subject><subject>Genetic Variation</subject><subject>ileum</subject><subject>Intestines - metabolism</subject><subject>Intestines - surgery</subject><subject>messenger RNA</subject><subject>pathogenesis</subject><subject>Protein Isoforms - metabolism</subject><subject>receptors</subject><subject>Receptors, Serotonin - genetics</subject><subject>Receptors, Serotonin - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>serotonin</subject><issn>0002-9645</issn><issn>1943-5681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU1v1DAQhi0EotvCjTP4xKlZPI7tJMeqfEoVSEDPlj8bV0kcbIeSP8NvJcuuBKeZ0Tx6NKMXoRdA9pQJ8kbd_0x70exh3_FHaAcdqysuWniMdoQQWnWC8TN0nvM9IUBb4E_RGQhGBYV2h36_DbmkoJcS4oSjx-PXz1fYRBumO-xjwrzqV5vir7WkdS5qDJPDyRk3l22ZF13W2WUcJlx6t5XictmQfFD1Tg2lX7FVIa2b8yFjNdn_x4dQemycUQO2YVBFHa6obMhDNH_7Z-iJV0N2z0_1At2-f_f9-mN18-XDp-urm8rUHErl2lY71tS6Jk4p4oFxcB1hxhLrBTUN860wFjTntFO0oU5bzxvdWs2p5qy-QK-P3jnFH8v2gxxDNm4Y1OTikmVDREehhg28PIImxZyT83JOYVRplUDkIQ95yEOKRoLs-Ia_PHkXPTr7Dz4FsAGvjoBXUaq7FLK8_UYJ1AQI56KB-g-6l5Si</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>Engel, L</creator><creator>Kobel, B</creator><creator>Ontsouka, E.C</creator><creator>Graber, H.U</creator><creator>Blum, J.W</creator><creator>Steiner, A</creator><creator>Meylan, M</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2006</creationdate><title>Distribution of mRNA coding for 5-hydroxytryptamine receptor subtypes in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation</title><author>Engel, L ; Kobel, B ; Ontsouka, E.C ; Graber, H.U ; Blum, J.W ; Steiner, A ; Meylan, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-e88be473b30eaa0f1451e904cd0df62c74f86cd1b5529a272ebdf57b8db52b543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Cattle</topic><topic>cattle diseases</topic><topic>Cattle Diseases - metabolism</topic><topic>cecal dilatation-dislocation disease</topic><topic>Cecal Diseases - metabolism</topic><topic>Cecal Diseases - veterinary</topic><topic>cecum</topic><topic>colon</topic><topic>dairy cows</topic><topic>digestive system diseases</topic><topic>Dilatation, Pathologic - veterinary</topic><topic>Female</topic><topic>gastrointestinal motility</topic><topic>gene expression</topic><topic>Genetic Variation</topic><topic>ileum</topic><topic>Intestines - metabolism</topic><topic>Intestines - surgery</topic><topic>messenger RNA</topic><topic>pathogenesis</topic><topic>Protein Isoforms - metabolism</topic><topic>receptors</topic><topic>Receptors, Serotonin - genetics</topic><topic>Receptors, Serotonin - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>serotonin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Engel, L</creatorcontrib><creatorcontrib>Kobel, B</creatorcontrib><creatorcontrib>Ontsouka, E.C</creatorcontrib><creatorcontrib>Graber, H.U</creatorcontrib><creatorcontrib>Blum, J.W</creatorcontrib><creatorcontrib>Steiner, A</creatorcontrib><creatorcontrib>Meylan, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Engel, L</au><au>Kobel, B</au><au>Ontsouka, E.C</au><au>Graber, H.U</au><au>Blum, J.W</au><au>Steiner, A</au><au>Meylan, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of mRNA coding for 5-hydroxytryptamine receptor subtypes in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation</atitle><jtitle>American journal of veterinary research</jtitle><addtitle>Am J Vet Res</addtitle><date>2006</date><risdate>2006</risdate><volume>67</volume><issue>1</issue><spage>95</spage><epage>101</epage><pages>95-101</pages><issn>0002-9645</issn><eissn>1943-5681</eissn><abstract>To investigate the distribution of mRNA coding for 7 subtypes of 5-hydroxytryptamine receptors (5-HTRs) in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation (CDD).
Full-thickness intestinal wall biopsy specimens were obtained from the ileum, cecum, proximal loop of the ascending colon, and external loop of the spiral colon (ELSC) of 15 cows with CDD (group 1) and 15 healthy dairy cows allocated to 2 control groups (specimens collected during routine laparotomy [group 2] or after cows were slaughtered [group 3]).
Amounts of mRNA coding for 7 subtypes of 5-HTRs (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1F, 5-HT2A, 5-HT2B, and 5-HT4) were measured by quantitative real-time reverse transcriptase-PCR assay. Results were expressed as the percentage of mRNA expression of a housekeeping gene.
Expression of mRNA coding for 5-HTR1B, 5-HTR2B, and 5-HTR4 was significantly lower in cows with CDD than in healthy cows. For 5-HTR2B and 5-HTR4, significant differences between cows with CDD and control cows were most pronounced for the ELSC. Expression of mRNA for 5-HTR1D, 5-HTR1F, and 5-HTR2A was extremely low in all groups, and mRNA for 5-HTR1A was not detected.
Relative concentrations of mRNA coding for 5-HTR1B, 5-HT2B, and 5-HTR4 were significantly lower in the intestines of cows with CDD than in the intestines of healthy dairy cows, especially for 5-HT2B and 5-HTR4 in the ELSC. This supports the hypothesis that serotonergic mechanisms, primarily in the spiral colon, are implicated in the pathogenesis of CDD.</abstract><cop>United States</cop><pmid>16426218</pmid><doi>10.2460/ajvr.67.1.95</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | American journal of veterinary research, 2006, Vol.67 (1), p.95-101 |
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| subjects | Analysis of Variance Animals Cattle cattle diseases Cattle Diseases - metabolism cecal dilatation-dislocation disease Cecal Diseases - metabolism Cecal Diseases - veterinary cecum colon dairy cows digestive system diseases Dilatation, Pathologic - veterinary Female gastrointestinal motility gene expression Genetic Variation ileum Intestines - metabolism Intestines - surgery messenger RNA pathogenesis Protein Isoforms - metabolism receptors Receptors, Serotonin - genetics Receptors, Serotonin - metabolism Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Messenger - genetics RNA, Messenger - metabolism serotonin |
| title | Distribution of mRNA coding for 5-hydroxytryptamine receptor subtypes in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation |
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